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AB277462

Human Serpin A5 ELISA Kit

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Human Serpin A5 ELISA Kit is a Sandwich ELISA for the measurement of Human Serpin A5 in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

PCI, PLANH3, PROCI, SERPINA5, Plasma serine protease inhibitor, Acrosomal serine protease inhibitor, Plasminogen activator inhibitor 3, Protein C inhibitor, Serpin A5, PAI-3, PAI3

1 Images
ELISA - Human Serpin A5 ELISA Kit (AB277462)
  • ELISA

Supplier Data

ELISA - Human Serpin A5 ELISA Kit (AB277462)

These standard curves are for demonstration only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 24 pg/mL

Range

24.58 - 6000 pg/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Primary Antibodies

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Proteins & Peptides

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Bioactive

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Serpin A5 ELISA Kit (ab277462) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Serpin A5 in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for Human Serpin A5 coated on a 96-well plate. Standards and samples are pipetted into the wells and Human Serpin A5 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human Serpin A5 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human Serpin A5 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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Recovery

[ { "sample": "Serum", "range": "80 - 89 %", "average": "= 85.61" }, { "sample": "Plasma", "range": "83 - 93 %", "average": "= 87.32" }, { "sample": "Cell culture media", "range": "67 - 80 %", "average": "= 72.11" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab277462-1x96Tests", "assetComponentDetails": [ { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB277462-CMP05", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB277462-CMP01", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB277462-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Human Serpin A5 coated Microplate", "number":"AB277462-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Human Serpin A5 Antibody", "number":"AB277462-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Serpin A5 standard protein (Lyophilized)", "number":"AB277462-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB277462-CMP07", "productcode":"" }, { "size":"1 x 200 µL", "name":"300X HRP-Streptavidin Concentrate", "number":"AB277462-CMP02", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Serpin A5 also known as Protein C Inhibitor (PCI) or Mesotrypsin inhibitor is a member of the serine protease inhibitor family with a mass of approximately 47 kDa. It plays a mechanical role as an inhibitor of several proteases such as trypsin tissue-type plasminogen activator and urokinase-type plasminogen activator. This protein is highly expressed in tissues including the liver kidneys and seminal plasma suggesting its involvement in reproductive and hemostatic processes.
Biological function summary

Serpin A5 regulates critical physiological processes through its inhibitory function. It interacts with and inhibits target proteases controlling pathways involved in coagulation and fibrinolysis. Serpin A5 does not form part of larger protein complexes but exerts its function through direct interactions. Its modulatory action impacts processes such as cell migration and tumor invasion highlighting its role in broader cellular environments.

Pathways

Serpin A5 is an important regulator in the coagulation cascade and fibrinolytic pathways. It acts to modulate the balance between coagulation and fibrinolysis critical for maintaining hemostasis. This protein is related to other serpins such as Antithrombin III and Plasminogen activator inhibitor-1 through its regulatory actions in these pathways. The inhibition of proteases by Serpin A5 ensures proper activation and termination of these biological processes which are essential for vascular integrity.

Serpin A5 is connected to conditions like thrombophilia and prostate cancer. Dysregulation in its expression or function leads to altered coagulation increasing the risk of thrombotic events. In prostate cancer its role is linked through pathways involving the matrix metalloproteinases where Serpin A5 can influence tumor progression and metastasis. Understanding its interactions with proteins in these diseases provides insights into potential therapeutic strategies.
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Product protocols

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Target data

Heparin-dependent serine protease inhibitor acting in body fluids and secretions. Inactivates serine proteases by binding irreversibly to their serine activation site. Involved in the regulation of intravascular and extravascular proteolytic activities. Plays hemostatic roles in the blood plasma. Acts as a procoagulant and pro-inflammatory factor by inhibiting the anticoagulant activated protein C factor as well as the generation of activated protein C factor by the thrombin/thrombomodulin complex. Acts as an anticoagulant factor by inhibiting blood coagulation factors like prothrombin, factor XI, factor Xa, plasma kallikrein and fibrinolytic enzymes such as tissue- and urinary-type plasminogen activators. In seminal plasma, inactivates several serine proteases implicated in the reproductive system. Inhibits the serpin acrosin; indirectly protects component of the male genital tract from being degraded by excessive released acrosin. Inhibits tissue- and urinary-type plasminogen activator, prostate-specific antigen and kallikrein activities; has a control on the sperm motility and fertilization. Inhibits the activated protein C-catalyzed degradation of SEMG1 and SEMG2; regulates the degradation of semenogelin during the process of transfer of spermatozoa from the male reproductive tract into the female tract. In urine, inhibits urinary-type plasminogen activator and kallikrein activities. Inactivates membrane-anchored serine proteases activities such as MPRSS7 and TMPRSS11E. Inhibits urinary-type plasminogen activator-dependent tumor cell invasion and metastasis. May also play a non-inhibitory role in seminal plasma and urine as a hydrophobic hormone carrier by its binding to retinoic acid.
See full target information SERPINA5
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websiteProtocolBooklet
en
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Product promise

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