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AB314341

Human Serpin B3/SCCA ELISA Kit

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Human Serpin B3/SCCA ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human Serpin B3/SCCA in Human in Cell Culture Media, Biofluids samples.

View Alternative Names

SCCA, SCCA1, SERPINB3, Serpin B3, Protein T4-A, Squamous cell carcinoma antigen 1, SCCA-1

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Sandwich ELISA - Human Serpin B3/SCCA ELISA Kit (AB314341)
  • sELISA

Supplier Data

Sandwich ELISA - Human Serpin B3/SCCA ELISA Kit (AB314341)

Example of standard curve using ab314341. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Plasma, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 0.15 ng/mL

Range

0.156 - 10 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Serpin B3/SCCA ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Serpin B3/SCCA in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human Serpin B3/SCCA coated on a 96-well plate.
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Recovery

[ { "sample": "Serum", "range": "71 - 140 %", "average": "= 111.2" }, { "sample": "Plasma", "range": "69 - 120 %", "average": "= 89.43" }, { "sample": "Cell culture media", "range": "118 - 141 %", "average": "= 127.4" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SerpinB3 also known as Squamous Cell Carcinoma Antigen (SCCA) is part of the serine protease inhibitor family. This protein has a molecular mass of approximately 45 kDa. It is expressed in various human tissues including skin liver and the respiratory epithelium. SerpinB3 acts by inhibiting cysteine proteinases through binding to them which helps regulate their activity and prevent excessive proteolysis. Such regulatory mechanisms are essential for maintaining cellular homeostasis.
Biological function summary

SerpinB3 plays a role in cellular proliferation differentiation and protection against apoptosis. It is not reported as part of a specific protein complex but it engages in interactions that influence cell signaling pathways. By protecting cells from programmed cell death SerpinB3 can contribute to tissue repair and regeneration. Its expression levels can change significantly during cell stress responses suggesting its involvement in adaptive processes to environmental stimuli.

Pathways

Protein SerpinB3 is involved in inflammatory and immune pathways. It influences the NF-κB signaling pathway which is important for immune response regulation and inflammation. This pathway involves multiple proteins including IκB which acts as an inhibitor and other cytokines and transcription factors that facilitate signal transduction in response to stress. SerpinB3’s modulation of these pathways reflects how it affects both innate and adaptive immune responses.

SerpinB3 has been associated with the progression of squamous cell carcinoma and liver fibrosis. In squamous cell carcinoma its interaction with proteases like cathepsin contributes to cancer cell survival and metastasis. For liver fibrosis SerpinB3’s inhibition of apoptosis in hepatocytes may lead to excessive tissue scarring. These connections illustrate its potential as a biomarker for diagnosis and as a target for therapeutic intervention.
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Product protocols

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Target data

May act as a papain-like cysteine protease inhibitor to modulate the host immune response against tumor cells. Also functions as an inhibitor of UV-induced apoptosis via suppression of the activity of c-Jun NH(2)-terminal kinase (JNK1).
See full target information SERPINB3
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Product promise

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