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AB314387

Human SerpinB4/SCCA-2 ELISA Kit

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Human SerpinB4/SCCA-2 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human SerpinB4/SCCA-2 in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

PI11, SCCA2, SERPINB4, Serpin B4, Leupin, Peptidase inhibitor 11, Squamous cell carcinoma antigen 2, PI-11, SCCA-2

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Sandwich ELISA - Human SerpinB4/SCCA-2 ELISA Kit (AB314387)
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Supplier Data

Sandwich ELISA - Human SerpinB4/SCCA-2 ELISA Kit (AB314387)

Example of standard curve using ab314387. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Serum, Plasma, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 0.23 ng/mL

Range

0.23 - 50 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human SerpinB4/SCCA-2 ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human SerpinB4/SCCA-2 in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human SerpinB4/SCCA-2 coated on a 96-well plate.

This ELISA kit shows no cross-reactivity with the following cytokines tested: human Adenosine Deaminase/ADA, AIF, AKR1C4 , ASAH2, BCL-2, BID, Calreticulin, Calreticulin-2, CD314 (NKG2D), CD39L3/ENTPD3, CD51, CD99-L2, CDC25B, Cerberus 1, CHST2, Cochlin, CRELD2, DC-SIGNR/CD299, eNOS, ENPP2/Autotaxin, FABP4, Fc epsilon RI, FGF R5, GALNT2, GALNT3, GIF, GPR111, beta-Glucuronidase/GUSB, Inhibin A, LILRB4/CD85k/ILT3, Neuroglycan C/CSPG5, NKp46/NCR1, Neuronal Pentraxin R/NPTXR, ROR2, Siglec-2/CD22, SIRP alpha/CD172a, SorCS1, Trypsin 1/PRSS1, Trypsin 3/PRSS3.

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Recovery

[ { "sample": "Serum", "range": "101 - 110 %", "average": "= 105.3" }, { "sample": "Plasma", "range": "103 - 125 %", "average": "= 113.2" }, { "sample": "Cell culture media", "range": "115 - 133 %", "average": "= 123" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SerpinB4 also known as SCCA-2 is a member of the serine protease inhibitor family. This protein weighs approximately 45 kDa. Physically it inhibits the activity of serine proteases by forming stable complexes with target enzymes therefore blocking their activity. SerpinB4 is expressed mainly in the epithelial cells particularly in tissues like the skin esophagus and cervix. It acts by modulating proteolytic processes important for maintaining tissue integrity.
Biological function summary

SerpinB4 plays a role in immune response regulation and protection against protease-mediated damage. It forms part of a regulatory network that controls inflammatory responses and cell death. The protein functions within a larger group of serpins involved in inhibiting distinct sets of proteases that influence processes such as apoptosis and inflammation. In keratinocytes SerpinB4 limits excessive proteolytic activity helping in response to environmental stressors and injuries.

Pathways

SerpinB4 interacts within the apoptosis and inflammatory response pathways. In the apoptosis pathway its function intersects with caspase signaling acting indirectly to counterbalance cell death signals. This protein is associated with tumor necrosis factor (TNF) pathways where it helps regulate the cascade of inflammatory cytokines. Through these interactions it shares functional pathways with other serpin proteins like SerpinB3 which also modulate inflammation and apoptosis.

SerpinB4 often implicates in cancers and inflammatory skin disorders. Its expression levels correlate with certain types of squamous cell carcinoma linking it to cancer progression and metastasis. Additionally alterations in SerpinB4 function can relate to atopic dermatitis a chronic inflammatory skin condition. In these contexts SerpinB4 interacts with other proteins such as SerpinB3 revealing a shared regulatory mechanism that influences both cancer and inflammatory diseases.
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Product protocols

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Target data

May act as a protease inhibitor to modulate the host immune response against tumor cells.
See full target information SERPINB4
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websiteProtocolBooklet
en
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Complementary Products

Primary Antibodies

AB255272

Anti-SerpinB3/SCCA + SerpinB4/SCCA-2 antibody [EPR22455-57] - BSA and Azide free

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SerpinB3/SCCA + SerpinB4/SCCA-2 antibody [EPR22455-57] - BSA and Azide free (AB255272)

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Proteins & Peptides

AB202597

Recombinant Human SerpinB4/SCCA-2 protein

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Product promise

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