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AB284024

Human SH3GL3 ELISA Kit

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Human SH3GL3 ELISA Kit is a Sandwich ELISA for the measurement of Human SH3GL3 in Human in Biofluids, Cell/Tissue Extracts samples.

View Alternative Names

CNSA3, SH3D2C, SH3GL3, Endophilin-A3, EEN-B2, Endophilin-3, SH3 domain protein 2C, SH3 domain-containing GRB2-like protein 3

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Functional Studies - Human SH3GL3 ELISA Kit (AB284024)
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Supplier Data

Functional Studies - Human SH3GL3 ELISA Kit (AB284024)

Human SH3GL3 ELISA Kit - Functional Studies.

Standard curve analysis of ab284024. For reference use only.

Key facts

Detection method

Colorimetric

Sample types

Cerebral Spinal Fluid, Cell Lysate

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 1.5 ng/mL

Range

1.875 - 120 ng/mL

Assay time

4h

Assay Platform

Microplate (12 x 8 well strips)

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Complementary Products

Primary Antibodies

AB184008

Anti-SH3GL3 antibody

Western blot - Anti-SH3GL3 antibody (AB184008)

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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Proteins & Peptides

AB202261

Recombinant Human SH3GL3 protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human SH3GL3 protein (His tag N-Terminus) (AB202261)

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  • 0
Primary Antibodies

AB137383

Anti-Carma 3 antibody

Western blot - Anti-Carma 3 antibody (AB137383)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human SH3GL3 ELISA Kit (ab284024) is designed for detection of SH3GL3 in human CSF and cell lysate samples. This assay employs a quantitative sandwich enzyme immunoassay technique that measures human SH3GL3 in approximately 4 hours.

A polyclonal antibody specific for human SH3GL3 has been pre-coated onto a 96-well microplate with removable strips. SH3GL3 in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for human SH3GL3, which is recognized by a streptavidinperoxidase (SP) conjugate. All unbound material is washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 60, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 6" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 60, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 10.5" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab284024-1x96Tests", "assetComponentDetails": [ { "size":"1 x 11 mL", "name":"Stop Solution", "number":"AB284024-CMP09", "productcode":"" }, { "size":"1 x 7 mL", "name":"Chromogen Substrate", "number":"AB284024-CMP06", "productcode":"" }, { "size":"1 x 120 µL", "name":"50X Biotinylated Human SH3GL3 Antibody", "number":"AB284024-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human SH3GL3 Microplate", "number":"AB284024-CMP04", "productcode":"" }, { "size":"1 x 80 µL", "name":"100X Streptavidin-Peroxidase Conjugate", "number":"AB284024-CMP10", "productcode":"" }, { "size":"2 x 30 mL", "name":"20X Wash Buffer Concentrate", "number":"AB284024-CMP07", "productcode":"" }, { "size":"1 x 2 mL", "name":"1X Standard Diluent", "number":"AB284024-CMP02", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Human SH3GL3 Standard", "number":"AB284024-CMP05", "productcode":"" }, { "size":"1 x 30 mL", "name":"10X Diluent N Concentrate", "number":"AB284024-CMP01", "productcode":"" }, { "size":"3 x 1 Unit", "name":"Sealing Tapes", "number":"AB284024-CMP08", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
Multi
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SH3GL3 also known as endophilin A3 is a protein with a molecular mass of approximately 40 kDa. It functions mechanically through its SH3 domain which facilitates interaction with proline-rich motifs in other proteins part of various cellular processes. Expressed mainly in the brain and neural tissues SH3GL3 plays significant roles in intracellular signaling and membrane dynamics. Its presence in neuronal tissue suggests an involvement in key neurological processes.
Biological function summary

SH3GL3 interacts with other proteins as part of the endophilin protein family involved in endocytosis and synaptic vesicle recycling. It acts as an adaptor linking intracellular signaling proteins to membrane-bound receptors influencing the way neurons communicate and adapt to changes. SH3GL3 helps regulate the formation and maintenance of synaptic connections ensuring proper neural network function.

Pathways

SH3GL3 participates in the regulation of the endocytosis pathway engaging in vital neuron communication mechanisms. It connects with proteins like dynamin and synaptojanin to modulate vesicle scission and recycling. Through the Ras signaling pathway it further affects synaptic plasticity and cellular responses to external stimuli supporting the adaptability of neural tissues.

SH3GL3 associates with neurological conditions including Alzheimer's disease and schizophrenia. In Alzheimer's altered interactions with proteins like amyloid precursor protein (APP) might disrupt synaptic function contributing to cognitive decline. In schizophrenia modifications in endocytic and signaling pathways involving SH3GL3 and proteins like neuregulin-1 could affect neurotransmission leading to symptoms commonly observed in the disorder.
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Product protocols

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Target data

Implicated in endocytosis. May recruit other proteins to membranes with high curvature (By similarity).
See full target information SH3GL3
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websiteProtocolBooklet
en
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Product promise

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