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AB314344

Human SHP2 ELISA Kit

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Human SHP2 ELISA Kit is a Sandwich ELISA for the measurement of Human SHP2 in Human in Cell Culture Media, Biofluids samples.

View Alternative Names

PTP2C, SHPTP2, PTPN11, Tyrosine-protein phosphatase non-receptor type 11, Protein-tyrosine phosphatase 1D, Protein-tyrosine phosphatase 2C, SH-PTP2, SH-PTP3, PTP-1D, PTP-2C, SHP-2, Shp2

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Sandwich ELISA - Human SHP2 ELISA Kit (AB314344)
  • sELISA

Supplier Data

Sandwich ELISA - Human SHP2 ELISA Kit (AB314344)

Example of standard curve using ab314344. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Plasma, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 1.52 ng/mL

Range

1.52 - 400 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Complementary Products

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AB32083

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RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHP2 antibody [Y478] (AB32083)

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Proteins & Peptides

AB268899

Recombinant human SHP2 protein (Active)

Functional Studies - Recombinant human SHP2 protein (Active) (AB268899)

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Assay Kits

AB279926

Phospho-SHP2 (Y542) and Total SHP2 ELISA Kit

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Assay Kits

AB206306

Mouse IL-27a ELISA Kit (Interleukin 27 p28 subunit)

Recombinant|SimpleStep

Sandwich ELISA - Mouse IL-27a ELISA Kit (Interleukin 27 p28 subunit) (AB206306)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human SHP2 ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human SHP2 in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human SHP2 coated on a 96-well plate.
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Recovery

[ { "sample": "Serum", "range": "101 - 110 %", "average": "= 105.3" }, { "sample": "Plasma", "range": "103 - 125 %", "average": "= 113.2" }, { "sample": "Cell culture media", "range": "115 - 133 %", "average": "= 123" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab314344-1x96Tests", "assetComponentDetails": [ { "size":"2 x 1 Vial", "name":"Biotinylated Human SHP2 Detection Antibody", "number":"AB314344-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human SHP2 Antibody-coated ELISA Plate", "number":"AB314344-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human SHP2 Protein Standard", "number":"AB314344-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB314344-CMP07", "productcode":"" }, { "size":"1 x 200 µL", "name":"100X HRP-Streptavidin", "number":"AB314344-CMP03", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB314344-CMP06", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent (Item E2)", "number":"AB314344-CMP05", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB314344-CMP04", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SHP2 also known as PTPN11 is a protein tyrosine phosphatase with a molecular mass of approximately 68 kDa. It is expressed in various tissues including the heart liver and immune cells. SHP2 belongs to the non-receptor class of protein tyrosine phosphatases and plays a critical role in cell signaling by acting as a regulator of signal transduction processes. SHP2 mediates these processes by dephosphorylating specific phosphotyrosine residues on target proteins influencing various cellular functions like proliferation differentiation and survival.
Biological function summary

The role of SHP2 extends to involvement in several signaling cascades such as the Ras/MAPK and PI3K/AKT pathways. It functions as an essential component within protein complexes that facilitate cell communication and response to external signals. The protein modulates growth factor signaling and cytokine signaling highlighting its significance in normal cell function and development. SHP2's statement in signaling processes makes it an important regulator of cellular dynamics.

Pathways

SHP2 participates in the Ras/MAPK and PI3K/AKT signaling pathways which are important for regulating cell growth survival and differentiation. Within these pathways SHP2 interacts with various signaling molecules including Grb2 Sos and Gab family adaptors. These interactions coordinate cellular responses to growth factors and other extracellular cues ensuring proper pathway activation and control. By serving as a critical mediator SHP2 integrates signals that are necessary for appropriate cellular outcomes.

SHP2 is associated with several conditions such as Noonan syndrome and various cancers. Mutations in the PTPN11 gene which encodes SHP2 often result in aberrant signaling that leads to developmental anomalies or tumorigenesis. In Noonan syndrome the mutated SHP2 protein results in disrupted Ras/MAPK pathway signaling. As for cancers SHP2 is often found to be overactive leading to enhanced cell proliferation and survival. In these contexts SHP2 is interconnected with other proteins like RAS and RAF which also contribute to oncogenic pathway activation and disease progression.
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Product protocols

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Target data

Acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus (PubMed : 10655584, PubMed : 14739280, PubMed : 18559669, PubMed : 18829466, PubMed : 26742426, PubMed : 28074573, PubMed : 32184441). Positively regulates MAPK signal transduction pathway (PubMed : 28074573). Dephosphorylates GAB1, ARHGAP35 and EGFR (PubMed : 28074573). Dephosphorylates ROCK2 at 'Tyr-722' resulting in stimulation of its RhoA binding activity (PubMed : 18559669). Dephosphorylates CDC73 (PubMed : 26742426). Dephosphorylates SOX9 on tyrosine residues, leading to inactivate SOX9 and promote ossification (By similarity). Dephosphorylates tyrosine-phosphorylated NEDD9/CAS-L (PubMed : 19275884). Acts as an effector of PDCD1-mediated inhibition of T-cell response : recruited by phosphorylated PDCD1, mediating dephosphorylation of key T-cell receptor (TCR) proximal signaling molecules, leading to TCR signaling inhibition (PubMed : 32184441).
See full target information PTPN11
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Product promise

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