AB322436

Human Sialoadhesin/CD169 ELISA Kit- Extracellular

Recombinant
SimpleStep
What is this?

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Human Sialoadhesin/CD169 ELISA Kit- Extracellular is a single-wash 90-min Simplestep used to quantify Human Sialoadhesin/CD169 with a sensitivity of 0.046 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders

View Alternative Names

CD169, SN, SIGLEC1, Sialoadhesin, Sialic acid-binding Ig-like lectin 1, Siglec-1

3 Images

Sandwich ELISA - Human Sialoadhesin/CD169 ELISA Kit- Extracellular (AB322436)

sELISA

Supplier Data

Sandwich ELISA - Human Sialoadhesin/CD169 ELISA Kit- Extracellular (AB322436)

Serum of ten individual healthy human male donors was measured in duplicate. Interpolated dilution factor corrected values (to neat samples) are plotted (mean +/- SD, n=2). The mean Sialoadhesin/CD169 concentration was determined to be 11.56 ng/mL with a range of 4.42 – 21.27 ng/mL.

sELISA

Supplier Data

Sandwich ELISA - Human Sialoadhesin/CD169 ELISA Kit- Extracellular (AB322436)

Interpolated concentration of native Sialoadhesin/CD169 was measured in duplicate at different sample concentrations. Undiluted samples are 50% serum, plasma (citrate), plasma (EDTA), and plasma (heparin). The interpolated dilution factor corrected values (to neat samples) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Supplier Data

Sandwich ELISA - Human Sialoadhesin/CD169 ELISA Kit- Extracellular (AB322436)

Example of human Sialoadhesin/CD169 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture media

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.046 ng/mL

Range

0.266 - 17 ng/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human Sialoadhesin/CD169 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Sialoadhesin/CD169 protein in Serum, Cit Plasma, EDTA Plasma, Hep Plasma, and Cell culture media. Quantitate Human Sialoadhesin/CD169 with 0.046 ng/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)

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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1.1" } ]

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Recovery

[ { "sample": "Serum", "range": "100 - 131 %", "average": "= 111" }, { "sample": "Citrate plasma", "range": "109 - 112 %", "average": "= 110" }, { "sample": "EDTA Plasma", "range": "98 - 122 %", "average": "= 108" }, { "sample": "Heparin Plasma", "range": "90 - 108 %", "average": "= 98" }, { "sample": "Cell culture media", "range": "100 - 113 %", "average": "= 106" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

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Target data

Macrophage-restricted adhesion molecule that mediates sialic-acid dependent binding to lymphocytes, including granulocytes, monocytes, natural killer cells, B-cells and CD8 T-cells. Plays a crucial role in limiting bacterial dissemination by engaging sialylated bacteria to promote effective phagocytosis and antigen presentation for the adaptive immune response (PubMed : 12940982, PubMed : 33489013). Mediates the uptake of various enveloped viruses via sialic acid recognition and subsequently induces the formation of intracellular compartments filled with virions (VCCs) (PubMed : 28129379). In turn, enhances macrophage-to-T-cell transmission of several viruses including HIV-1 or SARS-CoV-2 (PubMed : 28129379, PubMed : 34782760). Acts as an endocytic receptor mediating clathrin dependent endocytosis. Preferentially binds to alpha-2,3-linked sialic acid (PubMed : 12940982). Binds to SPN/CD43 on T-cells (By similarity). May play a role in hemopoiesis. Plays a role in the inhibition of antiviral innate immune by promoting TBK1 degradation via TYROBP and TRIM27-mediated ubiquitination (PubMed : 26358190).. (Microbial infection) Facilitates viral cytoplasmic entry into activated dendritic cells via recognition of sialylated gangliosides pesent on viral membrane.

See full target information SIGLEC1

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websiteProtocolBooklet

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