AB322435

Human Sialoadhesin/CD169 ELISA Kit- Intracellular

Recombinant
SimpleStep
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Human Sialoadhesin/CD169 ELISA Kit- Intracellular is a single-wash 90-min Simplestep used to quantify Human Sialoadhesin/CD169 with a sensitivity of 0.15 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput

View Alternative Names

CD169, SN, SIGLEC1, Sialoadhesin, Sialic acid-binding Ig-like lectin 1, Siglec-1

5 Images

Sandwich ELISA - Human Sialoadhesin/CD169 ELISA Kit- Intracellular (AB322435)

sELISA

Supplier Data

Sandwich ELISA - Human Sialoadhesin/CD169 ELISA Kit- Intracellular (AB322435)

Interpolated concentration of native Sialoadhesin/CD169 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 250 µg/mL THP-1 cell extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

sELISA

Supplier Data

Sandwich ELISA - Human Sialoadhesin/CD169 ELISA Kit- Intracellular (AB322435)

Interpolated concentration of native Sialoadhesin/CD169 was measured in duplicate at different sample concentrations. Undiluted samples are 250 µg/mL THP-1 cell extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

sELISA

Supplier Data

Sandwich ELISA - Human Sialoadhesin/CD169 ELISA Kit- Intracellular (AB322435)

Representative image of Human Sialoadhesin/CD169 ELISA Kit- Intracellular (ab322435)

Components shown from left to right :

- Sample diluent NS

- 10X Wash Buffer PT (ab206977)

- 10X Human Sialoadhesin/CD169 Detector Antibody

- Human Sialoadhesin/CD169 Lyophilised Purified Protein (2 vials)

- 50X Cell Extraction Enhancer Solution (ab193971)

- Human Sialoadhesin/CD169 Capture Antibody (Lyophilised)

- TMB solution

- Stop Solution

- 5X Cell Extraction Buffer PTR (ab193970)

- Antibody Diluent CPI2

SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.

Plate Seals - shown underneath bottles / vials.

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit

sELISA

Supplier Data

Sandwich ELISA - Human Sialoadhesin/CD169 ELISA Kit- Intracellular (AB322435)

Example of human Sialoadhesin/CD169 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human Sialoadhesin/CD169 ELISA Kit- Intracellular (AB322435)

Example of human Sialoadhesin/CD169 standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 0.15 ng/mL

Range

0.313 - 20 ng/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human Sialoadhesin/CD169 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Sialoadhesin/CD169 protein in Cell Lysate. Quantitate Human Sialoadhesin/CD169 with 0.15 ng/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)
-Also available in a fully validated 384-well format.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8.4" }, { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.2" } ]

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Recovery

[ { "sample": "Cell Lysate", "range": "99 - 110 %", "average": "= 104" } ]

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What's included?

{ "values": { "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab322435-10x96Tests", "assetComponentDetails": [ { "size":"1 x 120 mL", "name":"Stop Solution", "number":"AB322435-CMP04" }, { "size":"10 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB322435-CMP12" }, { "size":"1 x 120 mL", "name":"TMB Development Solution", "number":"AB322435-CMP03" }, { "size":"10 x 1 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB322435-CMP11" }, { "size":"10 x 600 µL", "name":"10X Human Sialoadhesin/CD169 Detector Antibody", "number":"AB322435-CMP08" }, { "size":"10 x 1 Vial", "name":"Lyophilized Human Sialoadhesin/CD169 Capture Antibody", "number":"AB322435-CMP01" }, { "size":"20 x 1 Vial", "name":"Human Sialoadhesin/CD169 Lyophilized Recombinant Protein", "number":"AB322435-CMP09" }, { "size":"10 x 1 Unit", "name":"Plate Seal", "number":"AB322435-CMP06" }, { "size":"10 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB322435-CMP05" }, { "size":"1 x 200 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB322435-CMP13" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB322435-CMP02" }, { "size":"10 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB322435-CMP10" } ] }, "1x384Tests": { "sellingSize": "1 x 384 Tests", "publicAssetCode":"ab322435-1x384Tests", "assetComponentDetails": [ { "size":"2 x 12 mL", "name":"Stop Solution", "number":"AB322435-CMP04" }, { "size":"1 x 5 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB322435-CMP12" }, { "size":"2 x 12 mL", "name":"TMB Development Solution", "number":"AB322435-CMP03" }, { "size":"1 x 50 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB322435-CMP11" }, { "size":"1 x 600 µL", "name":"10X Human Sialoadhesin/CD169 Detector Antibody", "number":"AB322435-CMP08" }, { "size":"1 x 1 Vial", "name":"Lyophilized Human Sialoadhesin/CD169 Capture Antibody", "number":"AB322435-CMP01" }, { "size":"2 x 1 Vial", "name":"Human Sialoadhesin/CD169 Lyophilized Recombinant Protein", "number":"AB322435-CMP09" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB322435-CMP02" }, { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB322435-CMP06" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB322435-CMP10" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB322435-CMP13" }, { "size":"1 x 1 Unit", "name":"384 well CaptSure™ microplates", "number":"AB322435-CMP07" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab322435-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB322435-CMP04", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB322435-CMP12", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB322435-CMP03", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB322435-CMP11", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Sialoadhesin/CD169 Detector Antibody", "number":"AB322435-CMP08", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Lyophilized Human Sialoadhesin/CD169 Capture Antibody", "number":"AB322435-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Sialoadhesin/CD169 Lyophilized Recombinant Protein", "number":"AB322435-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB322435-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB322435-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB322435-CMP06", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB322435-CMP10", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB322435-CMP13", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

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Target data

Macrophage-restricted adhesion molecule that mediates sialic-acid dependent binding to lymphocytes, including granulocytes, monocytes, natural killer cells, B-cells and CD8 T-cells. Plays a crucial role in limiting bacterial dissemination by engaging sialylated bacteria to promote effective phagocytosis and antigen presentation for the adaptive immune response (PubMed : 12940982, PubMed : 33489013). Mediates the uptake of various enveloped viruses via sialic acid recognition and subsequently induces the formation of intracellular compartments filled with virions (VCCs) (PubMed : 28129379). In turn, enhances macrophage-to-T-cell transmission of several viruses including HIV-1 or SARS-CoV-2 (PubMed : 28129379, PubMed : 34782760). Acts as an endocytic receptor mediating clathrin dependent endocytosis. Preferentially binds to alpha-2,3-linked sialic acid (PubMed : 12940982). Binds to SPN/CD43 on T-cells (By similarity). May play a role in hemopoiesis. Plays a role in the inhibition of antiviral innate immune by promoting TBK1 degradation via TYROBP and TRIM27-mediated ubiquitination (PubMed : 26358190).. (Microbial infection) Facilitates viral cytoplasmic entry into activated dendritic cells via recognition of sialylated gangliosides pesent on viral membrane.

See full target information SIGLEC1

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websiteProtocolBooklet

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Product promise

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