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AB267615

Human Siglec 7 ELISA Kit

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Human Siglec 7 ELISA Kit is a Sandwich ELISA for the measurement of Human Siglec 7 in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

CD328, AIRM1, SIGLEC7, Sialic acid-binding Ig-like lectin 7, Siglec-7, Adhesion inhibitory receptor molecule 1, CDw328, D-siglec, QA79 membrane protein, p75, AIRM-1

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Sandwich ELISA - Human Siglec 7 ELISA Kit (AB267615)
  • sELISA

Supplier Data

Sandwich ELISA - Human Siglec 7 ELISA Kit (AB267615)

Human Siglec 7 ELISA kit (ab267615) Standard curve.

Data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture media, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.1 ng/mL

Range

0.102 - 25 ng/mL

Assay Platform

Microplate

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Primary Antibodies

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Primary Antibodies

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Anti-MHC class I antibody [OX18]

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Primary Antibodies

AB108403

Anti-CD38 antibody [EPR4106]

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Siglec 7 ELISA Kit is designed for the quantitative determination of Siglec 7 in cell culture supernatants, plasma and serum samples.

This assay employs an antibody specific for human Siglec 7 coated on a 96-well plate. Standards and samples are pipetted into the wells and Siglec 7 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human Siglec 7 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Siglec 7 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

This ELISA kit shows no cross-reactivity with the following cytokines tested: human ADAMTS13, Aggrecan, Angiotensinogen (SERPINA8), B7-H1 (CD274), BMPR-IA (ALK-3), BMPR-II, Cadherin-11, CD27 (TNFRSF7), CD6, Ck beta 8-1 (CCL23), CNTF, DNAM-1 (CD226), CD147 (EMMPRIN), FLRG, Follistatin-like 1, Fractalkine (CX3CL1), Galectin-1, GITR Ligand (TNFSF18), Granulysin (NKG5/LAG-2), IL-1 R3 (IL-1 R Acp), IL-15 R alpha, IL-17E (IL-25), IL32 alpha, CHL-1 (L1CAM-2), LRIG3, LRP-6, MEPE (OF45), Nectin-4, Periostin, Persephin, Renin, RGM-B, ROBO3, S100 A8, Syndecan-3, Thrombospondin 2, Thrombospondin 5 (COMP), Tie-1, ULBP-2 (ALCAN-alpha/N2DL2).

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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Recovery

[ { "sample": "Serum", "range": "70 - 97 %", "average": "= 82" }, { "sample": "Plasma", "range": "68 - 93 %", "average": "= 78" }, { "sample": "Cell culture media", "range": "132 - 147 %", "average": "= 141" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab267615-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB267615-CMP07", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB267615-CMP04", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin Concentrate", "number":"AB267615-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Human Siglec 7 Detection Antibody", "number":"AB267615-CMP09", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB267615-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Human Siglec 7 coated Microplate (12 x 8 wells)", "number":"AB267615-CMP08", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent D", "number":"AB267615-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Siglec 7 Standard (Lyophilized)", "number":"AB267615-CMP01", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Substrate Solution", "number":"AB267615-CMP05", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,3- and alpha-2,6-linked sialic acid. Also binds disialogangliosides (disialogalactosyl globoside, disialyl lactotetraosylceramide and disialyl GalNAc lactotetraoslylceramide). The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Mediates inhibition of natural killer cells cytotoxicity. May play a role in hemopoiesis. Inhibits differentiation of CD34+ cell precursors towards myelomonocytic cell lineage and proliferation of leukemic myeloid cells (in vitro).
See full target information SIGLEC7
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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