Human Siglec 7 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human Siglec 7 in Human in Plasma, Cell culture media, Serum samples.
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture media, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 0.102 - 25 ng/mL
- Sensitivity
- = 0.1 ng/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,3- and alpha-2,6-linked sialic acid. Also binds disialogangliosides (disialogalactosyl globoside, disialyl lactotetraosylceramide and disialyl GalNAc lactotetraoslylceramide). The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Mediates inhibition of natural killer cells cytotoxicity. May play a role in hemopoiesis. Inhibits differentiation of CD34+ cell precursors towards myelomonocytic cell lineage and proliferation of leukemic myeloid cells (in vitro).
Alternative names
CD328, AIRM1, SIGLEC7, Sialic acid-binding Ig-like lectin 7, Siglec-7, Adhesion inhibitory receptor molecule 1, CDw328, D-siglec, QA79 membrane protein, p75, AIRM-1
What's included?
Recommended products
Human Siglec 7 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human Siglec 7 in Human in Plasma, Cell culture media, Serum samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture media, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 0.102 - 25 ng/mL
- Assay Platform
- Microplate
- Sensitivity
- = 0.1 ng/mL
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Overall | n 0 | C.V. < 10 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Overall | n 0 | C.V. < 12 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 82 | Range 70 - 97 % |
Sample type Plasma | Average % = 78 | Range 68 - 93 % |
Sample type Cell culture media | Average % = 141 | Range 132 - 147 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Human Siglec 7 ELISA Kit is designed for the quantitative determination of Siglec 7 in cell culture supernatants, plasma and serum samples.
This assay employs an antibody specific for human Siglec 7 coated on a 96-well plate. Standards and samples are pipetted into the wells and Siglec 7 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human Siglec 7 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Siglec 7 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
This ELISA kit shows no cross-reactivity with the following cytokines tested: human ADAMTS13, Aggrecan, Angiotensinogen (SERPINA8), B7-H1 (CD274), BMPR-IA (ALK-3), BMPR-II, Cadherin-11, CD27 (TNFRSF7), CD6, Ck beta 8-1 (CCL23), CNTF, DNAM-1 (CD226), CD147 (EMMPRIN), FLRG, Follistatin-like 1, Fractalkine (CX3CL1), Galectin-1, GITR Ligand (TNFSF18), Granulysin (NKG5/LAG-2), IL-1 R3 (IL-1 R Acp), IL-15 R alpha, IL-17E (IL-25), IL32 alpha, CHL-1 (L1CAM-2), LRIG3, LRP-6, MEPE (OF45), Nectin-4, Periostin, Persephin, Renin, RGM-B, ROBO3, S100 A8, Syndecan-3, Thrombospondin 2, Thrombospondin 5 (COMP), Tie-1, ULBP-2 (ALCAN-alpha/N2DL2).
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
Sandwich ELISA - Human Siglec 7 ELISA Kit (ab267615)
Human Siglec 7 ELISA kit (ab267615) Standard curve.
Data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com