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AB113339

Human Siglec 9 ELISA Kit (CD329)

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Human Siglec 9 ELISA Kit (CD329) is a Sandwich (quantitative) ELISA for the measurement of Human Siglec 9 (CD329) in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

CD329, UNQ668/PRO1302, SIGLEC9, Sialic acid-binding Ig-like lectin 9, Siglec-9, CDw329, Protein FOAP-9

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Sandwich ELISA - Human Siglec 9 ELISA Kit (CD329) (AB113339)
  • sELISA

Supplier Data

Sandwich ELISA - Human Siglec 9 ELISA Kit (CD329) (AB113339)

Representative Standard Curve using ab113339

Sandwich ELISA - Human Siglec 9 ELISA Kit (CD329) (AB113339)
  • sELISA

Supplier Data

Sandwich ELISA - Human Siglec 9 ELISA Kit (CD329) (AB113339)

Representative Standard Curve using ab113339

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

< 130 pg/mL

Range

0.123 - 30 ng/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's Siglec 9 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Siglec 9 in serum, plasma, cell culture supernatants.

This assay employs an antibody specific for Human Siglec 9 coated on a 96-well plate. Standards and samples are pipetted into the wells and Siglec 9 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Siglec 9 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Siglec 9 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Optimization may be required with urine samples.

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Recovery

[ { "sample": "Cell culture supernatant", "range": "88 - 102 %", "average": "= 95.43" }, { "sample": "Serum", "range": "72 - 91 %", "average": "= 82.7" }, { "sample": "Plasma", "range": "70 - 91 %", "average": "= 78.16" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab113339-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB113339-CMP03", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB113339-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Siglec 9 Microplate (12 x 8 wells)", "number":"AB113339-CMP01", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB113339-CMP09", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent D", "number":"AB113339-CMP06", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB113339-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Human Siglec 9", "number":"AB113339-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Human Siglec 9 Standard (lyophilized)", "number":"AB113339-CMP02", "productcode":"" }, { "size":"1 x 200 µL", "name":"500X HRP-Streptavidin Concentrate", "number":"AB113339-CMP04", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,3- or alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface.
See full target information SIGLEC9
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websiteProtocolBooklet
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Product promise

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