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AB267619

Human SIGLEC6 ELISA Kit

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Human SIGLEC6 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human SIGLEC6 in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

CD327, CD33L, CD33L1, OBBP1, SIGLEC6, Sialic acid-binding Ig-like lectin 6, Siglec-6, CD33 antigen-like 1, CDw327, Obesity-binding protein 1, OB-BP1

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Sandwich ELISA - Human SIGLEC6 ELISA Kit (AB267619)
  • sELISA

Supplier Data

Sandwich ELISA - Human SIGLEC6 ELISA Kit (AB267619)

This standard curve is for demonstration only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 0.42 ng/mL

Range

0.41 - 100 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human SIGLEC6 ELISA Kit is designed for the quantitative determination of SIGLEC6 in cell culture supernatants, plasma and serum samples.

Δ Note: Human SIGLEC6 concentration is low in normal serum/plasma and may not be detectable in this assay.

This assay employs an antibody specific for human SIGLEC6 coated on a 96-well plate. Standards and samples are pipetted into the wells and SIGLEC6 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human SIGLEC6 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of SIGLEC6 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

This ELISA kit shows no cross-reactivity with the following cytokines tested: Human Coagulation Factor X, CHMP2B, Contactin-3, Cortactin, CrkL, Cyr61/CCN1, DAPP1, Dynactin Subunit 1/DCTN1, DFF45/ICAD, DRAK1, GRAP2, GRK5, Hydroxyacid Oxidase1/HAO-1, LRIG1, MMP-12, NCK1, Nectin-2/CD112, Nesfatin1/Nucleobindin-2, Neurogranin, Nrf2, NUDT5, Nucleoporin NUP85, PAR1, Pancreatic Polypeptide/PP, Peroxiredoxin 2, PSMA1, PU.1/Spi-1, RalA, RCOR1/CoREST, Serpin B8, SH2D1A, SHP-1, SorCS3, THAP11, ULBP4/RAET1E, UNC5H3, VAMP-1, VAMP-2, PBEF/Visfatin.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" } ]
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Recovery

[ { "sample": "Serum", "range": "80 - 143 %", "average": "= 112" }, { "sample": "Plasma", "range": "71 - 134 %", "average": "= 94.83" }, { "sample": "Cell culture media", "range": "99 - 141 %", "average": "= 120.5" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab267619-1x96Tests", "assetComponentDetails": [ { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB267619-CMP02", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB267619-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Human SIGLEC6 coated Microplate (12 x 8 wells)", "number":"AB267619-CMP07", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB267619-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Substrate Solution", "number":"AB267619-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Human SIGLEC6 Detection Antibody", "number":"AB267619-CMP08", "productcode":"" }, { "size":"1 x 200 µL", "name":"400X HRP-Streptavidin Concentrate", "number":"AB267619-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human SIGLEC6 Standard (Lyophilized)", "number":"AB267619-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SIGLEC6 also known as CD327 is a protein found on some immune cells particularly on B cells and to a lesser extent on macrophages and dendritic cells. This protein has a molecular mass of around 100 kDa. As a member of the sialic acid-binding immunoglobulin-type lectins family (siglecs) SIGLEC6 mechanistically functions by recognizing and binding to specific sialic acid-containing carbohydrates on cell surfaces. This recognition influences cell-cell interactions and signaling processes important for immune response modulation.
Biological function summary

SIGLEC6 serves as an inhibitory receptor that helps dampen B cell receptor signaling balancing the immune response to prevent overactivation. It often partners with other proteins in complex cellular interactions within the immune cells. The expression of SIGLEC6 in placental tissues also suggests a role in trophoblast invasion and development although this function requires more research to fully understand the mechanisms involved.

Pathways

SIGLEC6 is a part of the B cell receptor signaling pathway acting as an inhibitory modulator. Through this pathway it connects with proteins like CD22 and other siglecs that contribute to the regulation of B cell activity. Additionally its role in glycan recognition links it to pathways associated with cell surface interactions and immune tolerance.

SIGLEC6 expression alterations have implications in chronic lymphocytic leukemia (CLL) where it potentially influences disease progression by modulating B cell signaling. The protein has also been connected to gestational trophoblastic diseases potentially affecting placental development. In these contexts SIGLEC6 interacts with proteins such as CD22 in CLL where they collectively affect leukemic cell behaviors and disease outcomes.
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Product protocols

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Target data

Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface.
See full target information SIGLEC6
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websiteProtocolBooklet
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