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AB326410

Human SIRP alpha (CD172a) ELISA Kit, Chemiluminescent

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Human SIRP alpha (CD172a) ELISA Kit, Chemiluminescent is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Human SIRP alpha (CD172a) with higher sensitivity and broader measuring range.

- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 54.87 - 40000 pg/ml with a sensitivity of 16.9pg/mL.
- Design your own immunoassay: we also offer the conjugation-ready antibody pair.
-Patent pending

View Alternative Names

CD172a, BIT, MFR, MYD1, PTPNS1, SHPS1, SIRP, SIRPA, Tyrosine-protein phosphatase non-receptor type substrate 1, SHP substrate 1, SHPS-1, Brain Ig-like molecule with tyrosine-based activation motifs, CD172 antigen-like family member A, Inhibitory receptor SHPS-1, Macrophage fusion receptor, MyD-1 antigen, Signal-regulatory protein alpha-1, Signal-regulatory protein alpha-2, Signal-regulatory protein alpha-3, p84, Bit, Sirp-alpha-1, Sirp-alpha-2, Sirp-alpha-3

4 Images
Sandwich ELISA - Human SIRP alpha (CD172a) ELISA Kit, Chemiluminescent (AB326410)
  • sELISA

Lab

Sandwich ELISA - Human SIRP alpha (CD172a) ELISA Kit, Chemiluminescent (AB326410)

SimpleStep ELISA® Ignite technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 55 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

Sandwich ELISA - Human SIRP alpha (CD172a) ELISA Kit, Chemiluminescent (AB326410)
  • sELISA

Lab

Sandwich ELISA - Human SIRP alpha (CD172a) ELISA Kit, Chemiluminescent (AB326410)

Standard curve comparison between the Chemiluminescent Human SIRP alpha SimpleStep ELISA and colorimetric Human SIRP alpha SimpleStep ELISA (ab272477) in 1X Cell Extraction Buffer PTR. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human SIRP alpha (CD172a) ELISA Kit, Chemiluminescent (AB326410)
  • sELISA

Lab

Sandwich ELISA - Human SIRP alpha (CD172a) ELISA Kit, Chemiluminescent (AB326410)

Dynamic range comparison between the Chemiluminescent Human SIRP alpha SimpleStep ELISA, colorimetric Human SIRP alpha SimpleStep ELISA (ab272477), and leading colorimetric competitor. The Chemiluminescent SimpleStep ELISA kit shows increased dynamic range.

Sandwich ELISA - Human SIRP alpha (CD172a) ELISA Kit, Chemiluminescent (AB326410)
  • sELISA

Supplier Data

Sandwich ELISA - Human SIRP alpha (CD172a) ELISA Kit, Chemiluminescent (AB326410)

Interpolated concentrations of native SIRP alpha in human U-937 cell extract.

based on a 250 µg/mL extract load SH-SY5Y cell extract based on a 2000 µg/mL extract load and brain tissue extract based on a 10 µg/mL extract load The concentrations of SIRP alpha were measured in duplicate and interpolated from the SIRP alpha standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD n=2). The mean SIRP alpha concentration was determined to be 7.08 ng/mL in U-937 extract and 3.03 ng/mL in SH-SY5Y extract and 6.85 ng/mL in brain extract.

Key facts

Detection method

Luminescent

Sample types

Tissue Extracts, Cell Lysate

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 16.9 pg/mL

Range

54.87 - 40000 pg/mL

Assay time

55m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human SIRP alpha (CD172a) ELISA Kit, Chemiluminescent (ab326410) is a rapid single-wash sandwich 55-min ELISA to measure Human SIRP alpha (CD172a) in Tissue Extracts, Cell Lysate with a sensitivity of 16.9 pg/mL.

How the assay works

Human SIRP alpha (CD172a) ELISA Kit, Chemiluminescent (ab326410) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human SIRP alpha (CD172a) (ab326410) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-SIRP alpha antibody [EPR22930-100] - BSA and Azide free (Capture) ab272765
- Anti-SIRP alpha antibody [EPR22930-23] - BSA and Azide free (Detector) ab272766
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Precision

[ { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 2.5" }, { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 5.2" } ]
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Recovery

[ { "sample": "Tissue Extracts", "range": "87 - 90 %", "average": "= 88" }, { "sample": "Cell Lysate", "range": "91 - 105 %", "average": "= 99" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab326410-1x96Tests", "assetComponentDetails": [ { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB326410-CMP05", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB326410-CMP06", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human SIRP alpha (CD172a) Detector Antibody", "number":"AB326410-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated Black 96-Well Microplate", "number":"AB326410-CMP10", "productcode":"" }, { "size":"1 x 3 mL", "name":"ChemiHRP Reagent B", "number":"AB326410-CMP09", "productcode":"" }, { "size":"1 x 3 mL", "name":"ChemiHRP Reagent A", "number":"AB326410-CMP08", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB326410-CMP04", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB326410-CMP07", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human SIRP alpha (CD172a) Capture Antibody", "number":"AB326410-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human SIRP alpha (CD172a) Lyophilized Recombinant Protein", "number":"AB326410-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB326410-CMP11", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Immunoglobulin-like cell surface receptor for CD47. Acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. Supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. May play a key role in intracellular signaling during synaptogenesis and in synaptic function (By similarity). Involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. Mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Plays a role in antiviral immunity and limits new world arenavirus infection by decreasing virus internalization (By similarity). Receptor for THBS1 (PubMed : 24511121). Interaction with THBS1 stimulates phosphorylation of SIRPA (By similarity). In response to THBS1, involved in ROS signaling in non-phagocytic cells, stimulating NADPH oxidase-derived ROS production (PubMed : 24511121).
See full target information SIRPA
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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