Human SIRP alpha ELISA Kit (CD172a)

Human SIRP ELISA kit (ab272477) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human SIRP protein in cell and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human SIRP with 76.90 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less

-High sensitivity, specificity and reproducibility from superior antibodies

-Fully validated in biological samples

-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

SIRP alpha (SIPRA or CD127a) is a 37.73 kD immunoglobulin-like cell surface receptor for CD47. SIRP alpha is involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors, or insulin. SIRP alpha may play a key role in intracellular signaling during synaptogenesis and in synaptic function (By similarity.) The antibodies are designed to a fragment of the extracellular domain of SIRP alpha.

Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.