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Human SIRT2 ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human SIRT2 in Cell culture extracts, Tissue Extracts, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma samples.

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Detection method

Colorimetric

Sample types

Cell culture extracts, Tissue Extracts, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

15.6 - 1000 pg/mL

Assay time

1h 30m

Sensitivity

= 3 pg/mL

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Reactivity data

Application
sELISA
Reactivity
Reacts
Dilution info
-
Notes

-

Images

Target data

What's included?

1 x 96 Tests
Components
10X Human SIRT2 Detector Antibody
1 x 600 µL
10X Wash Buffer PT (ab206977)
1 x 20 mL
50X Cell Extraction Enhancer Solution (ab193971)
1 x 1 mL
5X Cell Extraction Buffer PTR (ab193970)
1 x 10 mL
Antibody Diluent 4BR
1 x 6 mL
Human SIRT2 Capture Antibody (Lyophilized)
1 x 1 Vial
Human SIRT2 Lyophilized Recombinant Protein
2 x 1 Vial
Plate Seals
1 x 1 Unit
Sample Diluent NS (ab193972)
1 x 50 mL
SimpleStep Pre-Coated 96-Well Microplate (ab206978)
1 x 1 Unit
Stop Solution
1 x 12 mL
TMB Development Solution
1 x 12 mL

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Human SIRT2 ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human SIRT2 in Cell culture extracts, Tissue Extracts, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma samples.

Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts, Tissue Extracts, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

15.6 - 1000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

Sensitivity

= 3 pg/mL

Precision

Intra assay

Sample
Overall
n
5
mean
-
SD
-
C.V.
2.5

Inter assay

Sample
Overall
n
3
mean
-
SD
-
C.V.
4.1

Recovery

Sample specific recovery

Sample type
Serum
Average %
= 95
Range
93 - 96 %
Sample type
EDTA Plasma
Average %
= 84
Range
83 - 85 %
Sample type
Cell culture extracts
Average %
= 104
Range
93 - 116 %
Sample type
Tissue Extracts
Average %
= 108
Range
94 - 117 %
Sample type
Heparin Plasma
Average %
= 84
Range
82 - 86 %
Sample type
Citrate plasma
Average %
= 83
Range
80 - 84 %

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

Human SIRT2 ELISA Kit (ab227895) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of SIRT2 protein in cell culture extracts, cit plasma, edta plasma, hep plasma, serum, and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human SIRT2 with 3 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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