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Human SIRT2 ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human SIRT2 in Cell culture extracts, Tissue Extracts, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma samples.
Alternative names=NAD-dependent protein deacetylase sirtuin-2, Regulatory protein SIR2 homolog 2, SIR2-like protein 2, SIR2L, SIRT2, SIR2L2
Colorimetric
Cell culture extracts, Tissue Extracts, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma
Sandwich (quantitative)
Human
15.6 - 1000 pg/mL
1h 30m
= 3 pg/mL
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Human SIRT2 ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human SIRT2 in Cell culture extracts, Tissue Extracts, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma samples.
Alternative names=NAD-dependent protein deacetylase sirtuin-2, Regulatory protein SIR2 homolog 2, SIR2-like protein 2, SIR2L, SIRT2, SIR2L2
Colorimetric
Cell culture extracts, Tissue Extracts, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma
Sandwich (quantitative)
Human
15.6 - 1000 pg/mL
1h 30m
Pre-coated microplate (12 x 8 well strips)
= 3 pg/mL
Sample | n | mean | SD | C.V. |
---|---|---|---|---|
Sample Overall | n 5 | mean - | SD - | C.V. 2.5 |
Sample | n | mean | SD | C.V. |
---|---|---|---|---|
Sample Overall | n 3 | mean - | SD - | C.V. 4.1 |
Sample type | Average % | Range |
---|---|---|
Sample type Serum | Average % = 95 | Range 93 - 96 % |
Sample type EDTA Plasma | Average % = 84 | Range 83 - 85 % |
Sample type Cell culture extracts | Average % = 104 | Range 93 - 116 % |
Sample type Tissue Extracts | Average % = 108 | Range 94 - 117 % |
Sample type Heparin Plasma | Average % = 84 | Range 82 - 86 % |
Sample type Citrate plasma | Average % = 83 | Range 80 - 84 % |
Blue Ice
+4°C
+4°C
Human SIRT2 ELISA Kit (ab227895) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of SIRT2 protein in cell culture extracts, cit plasma, edta plasma, hep plasma, serum, and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human SIRT2 with 3 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
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