Human SIRT2 ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human SIRT2 in Cell culture extracts, Tissue Extracts, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma samples.
Colorimetric
Cell culture extracts, Tissue Extracts, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma
Sandwich (quantitative)
Human
15.6 - 1000 pg/mL
1h 30m
= 3 pg/mL
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Select an associated product type
NAD-dependent protein deacetylase, which deacetylates internal lysines on histone and alpha-tubulin as well as many other proteins such as key transcription factors (PubMed:24177535, PubMed:12620231, PubMed:16648462, PubMed:18249187, PubMed:18332217, PubMed:18995842, PubMed:20587414, PubMed:21081649, PubMed:20543840, PubMed:22014574, PubMed:21726808, PubMed:21949390, PubMed:22771473, PubMed:23468428, PubMed:23908241, PubMed:24940000, PubMed:24769394, PubMed:24681946). Participates in the modulation of multiple and diverse biological processes such as cell cycle control, genomic integrity, microtubule dynamics, cell differentiation, metabolic networks, and autophagy. Plays a major role in the control of cell cycle progression and genomic stability. Functions in the antephase checkpoint preventing precocious mitotic entry in response to microtubule stress agents, and hence allowing proper inheritance of chromosomes. Positively regulates the anaphase promoting complex/cyclosome (APC/C) ubiquitin ligase complex activity by deacetylating CDC20 and FZR1, then allowing progression through mitosis. Associates both with chromatin at transcriptional start sites (TSSs) and enhancers of active genes. Plays a role in cell cycle and chromatin compaction through epigenetic modulation of the regulation of histone H4 'Lys-20' methylation (H4K20me1) during early mitosis. Specifically deacetylates histone H4 at 'Lys-16' (H4K16ac) between the G2/M transition and metaphase enabling H4K20me1 deposition by KMT5A leading to ulterior levels of H4K20me2 and H4K20me3 deposition throughout cell cycle, and mitotic S-phase progression (PubMed:23468428). Deacetylates KMT5A modulating KMT5A chromatin localization during the mitotic stress response (PubMed:23468428). Deacetylates also histone H3 at 'Lys-57' (H3K56ac) during the mitotic G2/M transition. Upon bacterium Listeria monocytogenes infection, deacetylates 'Lys-18' of histone H3 in a receptor tyrosine kinase MET- and PI3K/Akt-dependent manner, thereby inhibiting transcriptional activity and promoting late stages of listeria infection (PubMed:23908241). During oocyte meiosis progression, may deacetylate histone H4 at 'Lys-16' (H4K16ac) and alpha-tubulin, regulating spindle assembly and chromosome alignment by influencing microtubule dynamics and kinetochore function. Deacetylates histone H4 at 'Lys-16' (H4K16ac) at the VEGFA promoter and thereby contributes to regulate expression of VEGFA, a key regulator of angiogenesis (PubMed:24940000). Deacetylates alpha-tubulin at 'Lys-40' and hence controls neuronal motility, oligodendroglial cell arbor projection processes and proliferation of non-neuronal cells. Phosphorylation at Ser-368 by a G1/S-specific cyclin E-CDK2 complex inactivates SIRT2-mediated alpha-tubulin deacetylation, negatively regulating cell adhesion, cell migration and neurite outgrowth during neuronal differentiation. Deacetylates PARD3 and participates in the regulation of Schwann cell peripheral myelination formation during early postnatal development and during postinjury remyelination. Involved in several cellular metabolic pathways. Plays a role in the regulation of blood glucose homeostasis by deacetylating and stabilizing phosphoenolpyruvate carboxykinase PCK1 activity in response to low nutrient availability. Acts as a key regulator in the pentose phosphate pathway (PPP) by deacetylating and activating the glucose-6-phosphate G6PD enzyme, and therefore, stimulates the production of cytosolic NADPH to counteract oxidative damage. Maintains energy homeostasis in response to nutrient deprivation as well as energy expenditure by inhibiting adipogenesis and promoting lipolysis. Attenuates adipocyte differentiation by deacetylating and promoting FOXO1 interaction to PPARG and subsequent repression of PPARG-dependent transcriptional activity. Plays a role in the regulation of lysosome-mediated degradation of protein aggregates by autophagy in neuronal cells. Deacetylates FOXO1 in response to oxidative stress or serum deprivation, thereby negatively regulating FOXO1-mediated autophagy (PubMed:20543840). Deacetylates a broad range of transcription factors and co-regulators regulating target gene expression. Deacetylates transcriptional factor FOXO3 stimulating the ubiquitin ligase SCF(SKP2)-mediated FOXO3 ubiquitination and degradation (By similarity). Deacetylates HIF1A and therefore promotes HIF1A degradation and inhibition of HIF1A transcriptional activity in tumor cells in response to hypoxia (PubMed:24681946). Deacetylates RELA in the cytoplasm inhibiting NF-kappaB-dependent transcription activation upon TNF-alpha stimulation. Inhibits transcriptional activation by deacetylating p53/TP53 and EP300 (PubMed:18249187). Deacetylates also EIF5A (PubMed:22771473). Functions as a negative regulator on oxidative stress-tolerance in response to anoxia-reoxygenation conditions. Plays a role as tumor suppressor (PubMed:22014574).Isoform 1Deacetylates EP300, alpha-tubulin and histone H3 and H4.Isoform 2Deacetylates EP300, alpha-tubulin and histone H3 and H4.Isoform 5Lacks deacetylation activity.
NAD-dependent protein deacetylase sirtuin-2, Regulatory protein SIR2 homolog 2, SIR2-like protein 2, SIR2L, SIRT2, SIR2L2
Human SIRT2 ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human SIRT2 in Cell culture extracts, Tissue Extracts, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma samples.
NAD-dependent protein deacetylase sirtuin-2, Regulatory protein SIR2 homolog 2, SIR2-like protein 2, SIR2L, SIRT2, SIR2L2
Colorimetric
Cell culture extracts, Tissue Extracts, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma
Sandwich (quantitative)
Human
15.6 - 1000 pg/mL
1h 30m
Pre-coated microplate (12 x 8 well strips)
= 3 pg/mL
Sample | n | mean | SD | C.V. |
---|---|---|---|---|
Sample Overall | n 5 | mean - | SD - | C.V. 2.5 |
Sample | n | mean | SD | C.V. |
---|---|---|---|---|
Sample Overall | n 3 | mean - | SD - | C.V. 4.1 |
Sample type | Average % | Range |
---|---|---|
Sample type Serum | Average % = 95 | Range 93 - 96 % |
Sample type EDTA Plasma | Average % = 84 | Range 83 - 85 % |
Sample type Cell culture extracts | Average % = 104 | Range 93 - 116 % |
Sample type Tissue Extracts | Average % = 108 | Range 94 - 117 % |
Sample type Heparin Plasma | Average % = 84 | Range 82 - 86 % |
Sample type Citrate plasma | Average % = 83 | Range 80 - 84 % |
Blue Ice
+4°C
+4°C
Human SIRT2 ELISA Kit (ab227895) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of SIRT2 protein in cell culture extracts, cit plasma, edta plasma, hep plasma, serum, and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human SIRT2 with 3 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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This supplementary information is collated from multiple sources and compiled automatically.
SIRT2 also known as Sirtuin 2 is a member of the sirtuin family of proteins which are NAD+-dependent deacetylases known for regulating various cellular processes. SIRT2 has a molecular mass of approximately 43 kDa. Its expression is prominent in the cytoplasm but can also be found in the nucleus of cells particularly in the brain and liver tissues. The protein deacetylates various substrates influencing cellular processes including aging metabolism and neurodegeneration.
SIRT2 contributes to the regulation of cellular homeostasis and stress responses. This protein acts as a deacetylase modulating the acetylation status of key proteins and participates in various signaling pathways. It functions as part of a larger protein complex and interacts with substrates that include tubulin and histones. These interactions help SIRT2 control processes such as cell cycle differentiation and proliferation indicating its importance in maintaining normal cellular function.
SIRT2 plays an essential role in the insulin signaling pathway and the FoxO transcription factor pathway. In these pathways SIRT2 interacts with other sirtuins like SIRT1 which helps regulate metabolic homeostasis and oxidative stress responses. SIRT2's activity in deacetylating proteins such as p53 impacts cellular responses to DNA damage and genomic stability further integrating it into critical regulatory networks within the cell.
SIRT2 relates to neurodegenerative diseases like Parkinson's disease and metabolic disorders such as obesity. Its involvement in deacetylating alpha-synuclein links SIRT2 to Parkinson's disease pathogenesis. In metabolic disorders abnormalities in SIRT2 activity can affect glucose and lipid metabolism connecting it with proteins like acetyl-CoA carboxylase. Understanding SIRT2's function offers potential therapeutic targets for these conditions and highlights its importance in disease regulation.
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Example of Human SIRT2 standard curve in 1X Cell Extraction Buffer PTR.
Background-subtracted data values (mean +/- SD) are graphed.
Example of Human SIRT2 standard curve in Sample Diluent NS.
Background-subtracted data values (mean +/- SD) are graphed.
Interpolated concentrations of native SIRT2 in Human Fetal Brain Homogenate Extract, U-87 MG Extract, PC-3 Extract, and THP-1 Extract.
Interpolated concentrations of native SIRT2 based on a 150 μg/ml extract load in Human Fetal Brain Homogenate Extract, 600 μg/ml in U-87 MG Extract, 250 μg/ml in PC-3 Extract, and 125 μg/ml in THP-1 Extract. The concentrations of SIRT2 were measured in duplicate and interpolated from the SIRT2 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean SIRT2 concentration was determined to be 998 pg/mL in Human Fetal Brain Homogenate Extract, 862 pg/mL in U-87 MG Extract, 978 pg/ml in PC-3 Extract, and 925 pg/ml in THP-1 Extract.
Interpolated concentrations of spiked SIRT2 in Human serum and plasma samples.
The concentrations of SIRT2 were measured in duplicates, interpolated from the SIRT2 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 25%, plasma (citrate) 25%, plasma (heparin) 25%, and plasma (EDTA) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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