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AB314347

Human SIRT3 ELISA Kit

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Human SIRT3 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human SIRT3 in Human in Cell Culture Media, Biofluids samples.

View Alternative Names

SIR2L3, SIRT3, hSIRT3, NAD-dependent protein delactylase sirtuin-3, Regulatory protein SIR2 homolog 3, SIR2-like protein 3

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Sandwich ELISA - Human SIRT3 ELISA Kit (AB314347)
  • sELISA

Supplier Data

Sandwich ELISA - Human SIRT3 ELISA Kit (AB314347)

Example of standard curve using ab314347. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Plasma, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 2.1 ng/mL

Range

2.1 - 500 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human SIRT3 ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human SIRT3 in serum (human SIRT3 concentration is low in normal serum/plasma, and may not be detectable in this assay concentration), plasma and cell culture supernatants. This assay employs an antibody specific for human SIRT3 coated on a 96-well plate.
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Recovery

[ { "sample": "Serum", "range": "95 - 118 %", "average": "= 108.5" }, { "sample": "Plasma", "range": "72 - 81 %", "average": "= 77.96" }, { "sample": "Cell culture media", "range": "81 - 91 %", "average": "= 85.65" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab314347-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Human SIRT3 Antibody-coated ELISA Plate", "number":"AB314347-CMP09", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB314347-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human SIRT3 Protein Standard", "number":"AB314347-CMP11", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB314347-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB314347-CMP08", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B (Item E)", "number":"AB314347-CMP06", "productcode":"" }, { "size":"1 x 200 µL", "name":"400X HRP-Streptavidin", "number":"AB314347-CMP03", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A (Item D)", "number":"AB314347-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human SIRT3 Detection Antibody", "number":"AB314347-CMP10", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SIRT3 also known as Sirtuin 3 and mitochondrial sirtuin is a deacetylase with a mass of roughly 44 kDa. This protein is mostly found in the mitochondria where it regulates the acetylation state of various substrates. It plays an important role in maintaining mitochondrial homeostasis by modifying protein activity. SIRT3 is evolutionarily conserved and highly expressed in tissues with high energy demand like the heart liver and brown adipose tissue.
Biological function summary

SIRT3 modulates energy homeostasis by deacetylating and regulating enzymes involved in metabolism. It is a part of the mitochondrial sirtuin family contributing to fatty acid oxidation and the antioxidant defense system. By targeting key metabolic enzymes SIRT3 directly impacts the tricarboxylic acid (TCA) cycle and oxidative phosphorylation processes. These functions highlight its role in maintaining energy efficiency and reducing reactive oxidative species.

Pathways

SIRT3 participates in vital metabolic pathways such as the TCA cycle and the urea cycle. It interacts with other proteins like MnSOD and IDH2 to enhance mitochondrial function and overall cellular metabolism. This helps control the balance between energy production and consumption integrating signals from the AMPK and NAD+-dependent pathways to coordinate responses to changes in energy availability.

SIRT3 has implications in metabolic syndrome and cancer. Reduced SIRT3 activity is associated with increased susceptibility to metabolic disturbances like obesity and type 2 diabetes. Its deficiency affects the regulation of ROS and metabolic adaptation linking it to tumorigenesis and cancer progression. As it connects with proteins like HIF-1α in hypoxic conditions SIRT3 plays a role in cellular adaptation to stress influencing disease progression.
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Product protocols

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Target data

NAD-dependent protein deacetylase (PubMed : 12186850, PubMed : 12374852, PubMed : 16788062, PubMed : 18680753, PubMed : 18794531, PubMed : 19535340, PubMed : 23283301, PubMed : 24121500, PubMed : 24252090). Activates or deactivates mitochondrial target proteins by deacetylating key lysine residues (PubMed : 12186850, PubMed : 12374852, PubMed : 16788062, PubMed : 18680753, PubMed : 18794531, PubMed : 23283301, PubMed : 24121500, PubMed : 24252090). Known targets include ACSS1, IDH, GDH, SOD2, PDHA1, LCAD, SDHA and the ATP synthase subunit ATP5PO (PubMed : 16788062, PubMed : 18680753, PubMed : 19535340, PubMed : 24121500, PubMed : 24252090). Contributes to the regulation of the cellular energy metabolism (PubMed : 24252090). Important for regulating tissue-specific ATP levels (PubMed : 18794531). In response to metabolic stress, deacetylates transcription factor FOXO3 and recruits FOXO3 and mitochondrial RNA polymerase POLRMT to mtDNA to promote mtDNA transcription (PubMed : 23283301). Acts as a regulator of ceramide metabolism by mediating deacetylation of ceramide synthases CERS1, CERS2 and CERS6, thereby increasing their activity and promoting mitochondrial ceramide accumulation (By similarity). Regulates hepatic lipogenesis (By similarity). Uses NAD(+) substrate imported by SLC25A47, triggering downstream activation of PRKAA1/AMPK-alpha signaling cascade that ultimately downregulates sterol regulatory element-binding protein (SREBP) transcriptional activities and ATP-consuming lipogenesis to restore cellular energy balance (By similarity). In addition to protein deacetylase activity, also acts as a protein-lysine deacylase by mediating delactylation of proteins, such as CCNE2 and 'Lys-16' of histone H4 (H4K16la) (PubMed : 36896611, PubMed : 37720100).
See full target information SIRT3
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