JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB314348

Human SIRT5 ELISA Kit

Be the first to review this product! Submit a review

|

(0 Publication)

Human SIRT5 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human SIRT5 in Human in Cell Culture Media, Biofluids samples.

View Alternative Names

SIR2L5, SIRT5, Regulatory protein SIR2 homolog 5, SIR2-like protein 5

1 Images
Sandwich ELISA - Human SIRT5 ELISA Kit (AB314348)
  • sELISA

Supplier Data

Sandwich ELISA - Human SIRT5 ELISA Kit (AB314348)

Example of standard curve using ab314348. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Plasma, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 0.12 ng/mL

Range

0.12 - 25 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Human SIRT5 ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human SIRT5 in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human SIRT5 coated on a 96-well plate.

This ELISA kit shows no cross-reactivity with the following cytokines tested: human Activin RIA, ASAHL, B4GalT1, BAI1, Brorin, C1qTNF4, CA14, CA4, CA6, CA8, Cadherin-6, Caspr2, CD27 Ligand, CD300a, CD300e, CD300f, CD4, CD5, CD69, CK18, CK19, CPB1, CRISP-2, DDR1, FUT8, MIA, NTAL, NTB-A, OMgp, PEAR1, Podoplanin, PTH1R, Reg4, ROR1, Semaphorin 4G, Serpin A5, Serpin B6, Siglec-1, Sirtuin 2.

style
left-column

Recovery

[ { "sample": "Serum", "range": "99 - 115 %", "average": "= 106.3" }, { "sample": "Plasma", "range": "83 - 115 %", "average": "= 101.8" }, { "sample": "Cell culture media", "range": "102 - 119 %", "average": "= 108.3" } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab314348-1x96Tests", "assetComponentDetails": [ { "size":"2 x 1 Vial", "name":"Lyophilized Human SIRT5 Protein Standard", "number":"AB314348-CMP10", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB314348-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human SIRT5 Antibody-coated ELISA Plate", "number":"AB314348-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB314348-CMP07", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin", "number":"AB314348-CMP03", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent (Item E2)", "number":"AB314348-CMP05", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB314348-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human SIRT5 Detection Antibody", "number":"AB314348-CMP09", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SIRT5 also known as Sirtuin 5 is a mitochondrial protein with enzymatic activity as a lysine desuccinylase demalonylase and deglutarylase. It has a molecular mass of approximately 34 kDa. This protein is expressed highly in tissues with high metabolic activity like the liver heart and brain where it participates in regulating energy metabolism. The activity of SIRT5 affects cellular processes by removing post-translational modifications from lysine residues modulating proteins' functions and interactions in these tissues.
Biological function summary

SIRT5 functions as an important regulator of mitochondrial metabolism and influences cellular energy homeostasis. It is not part of larger complexes but it interacts with various metabolic enzymes to control their function. Through its deacylase activity SIRT5 affects the urea cycle and fatty acid oxidation by modifying key enzymes involved in these processes. Its role in maintaining a balance in energy production and consumption highlights its importance in cellular metabolism.

Pathways

SIRT5 is involved in both the tricarboxylic acid (TCA) cycle and the regulation of reactive oxygen species (ROS). Within the TCA cycle SIRT5 desuccinylates and activates enzymes like citrate synthase enhancing the cycle's efficiency. In regulating ROS SIRT5 reduces oxidative stress by affecting enzymes such as superoxide dismutase 2 (SOD2). Its interaction with these pathways and proteins reflects its role in maintaining metabolic health and cellular integrity.

Alterations in SIRT5 levels have been connected to metabolic diseases such as obesity and neurodegenerative disorders like Alzheimer's disease. In obesity SIRT5 dysregulation can lead to inefficient metabolic processes and energy imbalance. In Alzheimer’s decreased SIRT5 activity may contribute to increased mitochondrial oxidative stress implicated in disease progression. These connections highlight SIRT5’s significance in both metabolic and neurodegenerative contexts linking with proteins like AMPK in obesity and tau proteins in Alzheimer's disease.
style
left-column

Product protocols

style
left-column

Target data

NAD-dependent lysine demalonylase, desuccinylase and deglutarylase that specifically removes malonyl, succinyl and glutaryl groups on target proteins (PubMed : 21908771, PubMed : 22076378, PubMed : 24703693, PubMed : 29180469). Activates CPS1 and contributes to the regulation of blood ammonia levels during prolonged fasting : acts by mediating desuccinylation and deglutarylation of CPS1, thereby increasing CPS1 activity in response to elevated NAD levels during fasting (PubMed : 22076378, PubMed : 24703693). Activates SOD1 by mediating its desuccinylation, leading to reduced reactive oxygen species (PubMed : 24140062). Activates SHMT2 by mediating its desuccinylation (PubMed : 29180469). Modulates ketogenesis through the desuccinylation and activation of HMGCS2 (By similarity). Has weak NAD-dependent protein deacetylase activity; however this activity may not be physiologically relevant in vivo. Can deacetylate cytochrome c (CYCS) and a number of other proteins in vitro such as UOX.
See full target information SIRT5
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Complementary Products

Primary Antibodies

AB259967

Anti-SIRT5 antibody [EPR23787-116]

KO Validated|RabMAb|Recombinant|Trial Size

Western blot - Anti-SIRT5 antibody [EPR23787-116] (AB259967)

  • 0
  • 0
Proteins & Peptides

AB101133

Recombinant Human SIRT5 protein

SDS-PAGE - Recombinant Human SIRT5 protein (AB101133)

  • 0
  • 0
Cell Lines & Lysates

AB282634

Human SIRT5 knockout A549 cell line

Advanced Validation

Western blot - Human SIRT5 knockout A549 cell line (AB282634)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com