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AB277429

Human SLAM7/CS1 ELISA Kit

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Human SLAM7/CS1 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human SLAM7/CS1 in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

CD319, CS1, UNQ576/PRO1138, SLAMF7, SLAM family member 7, CD2 subset 1, CD2-like receptor-activating cytotoxic cells, Membrane protein FOAP-12, Novel Ly9, Protein 19A, CRACC

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ELISA - Human SLAM7/CS1 ELISA Kit (AB277429)
  • ELISA

Supplier Data

ELISA - Human SLAM7/CS1 ELISA Kit (AB277429)

These standard curves are for demonstration only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 0.83 ng/mL

Range

0.819 - 200 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human SLAM7/CS1 ELISA Kit (ab277429) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human SLAM7/CS1 in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for Human SLAM7/CS1 coated on a 96-well plate. Standards and samples are pipetted into the wells and Human SLAM7/CS1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human SLAM7/CS1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human SLAM7/CS1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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Recovery

[ { "sample": "Serum", "range": "94 - 139 %", "average": "= 118.8" }, { "sample": "Plasma", "range": "93 - 143 %", "average": "= 119.1" }, { "sample": "Cell culture media", "range": "89 - 114 %", "average": "= 102.4" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SLAMF7 also known as CS1 is a glycoprotein with a mass of approximately 70 kDa. This protein localizes on the surface of immune cells mainly on natural killer (NK) cells certain subsets of T cells and mature dendritic cells. It does not express in resting B cells but can be induced upon activation. CS1 plays a role in immune modulation by facilitating cell-to-cell interactions and activating various signaling pathways that enable immune responses.
Biological function summary

SLAMF7/CS1 modulates immune functions by engaging in interactions with other cell surface receptors. It often operates in the context of hematopoietic cells integrating signals that influence cell proliferation differentiation and survival. It does not require an associated adaptor molecule like most of the SLAM family members as it signals through its ability to recruit EAT-2. The CS1 protein modulates pathways related to immune cell activation and control making it an important player in the regulation of immune responses.

Pathways

CS1 participates prominently in immune signaling pathways where it interacts with proteins such as SAP and EAT-2. It is particularly involved in the immunoregulatory interactions contributing to NK cell-mediated cytotoxicity and modulation of T-cell responses. These interactions illustrate CS1's role in the modulation of immune responses positioning it within the broader network of immune signaling pathways important for maintaining immune homeostasis and defense mechanisms.

SLAMF7/CS1 shows key relevance in multiple myeloma and systemic lupus erythematosus (SLE). In multiple myeloma CS1 presents an overexpression and serves as a therapeutic target; its relationship with CD38 another important marker illustrates the potential for targeted therapies. In SLE improper signaling involving CS1 can contribute to the dysregulation of immune responses. Overall the intricate interactions of CS1 with other proteins highlight its significance in disease pathology and therapeutic potential.
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Product protocols

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Target data

Self-ligand receptor of the signaling lymphocytic activation molecule (SLAM) family. SLAM receptors triggered by homo- or heterotypic cell-cell interactions are modulating the activation and differentiation of a wide variety of immune cells and thus are involved in the regulation and interconnection of both innate and adaptive immune response. Activities are controlled by presence or absence of small cytoplasmic adapter proteins, SH2D1A/SAP and/or SH2D1B/EAT-2. Isoform 1 mediates NK cell activation through a SH2D1A-independent extracellular signal-regulated ERK-mediated pathway (PubMed : 11698418). Positively regulates NK cell functions by a mechanism dependent on phosphorylated SH2D1B. Downstream signaling implicates PLCG1, PLCG2 and PI3K (PubMed : 16339536). In addition to heterotypic NK cells-target cells interactions also homotypic interactions between NK cells may contribute to activation. However, in the absence of SH2D1B, inhibits NK cell function. Acts also inhibitory in T-cells (By similarity). May play a role in lymphocyte adhesion (PubMed : 11802771). In LPS-activated monocytes negatively regulates production of pro-inflammatory cytokines (PubMed : 23695528).. Isoform 3 does not mediate any NK cell activation.
See full target information SLAMF7
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websiteProtocolBooklet
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