AB283885

Human Slit2 ELISA Kit

Recombinant
SimpleStep
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Human Slit2 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Slit2 with a sensitivity of 16.9 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

SLIL3, SLIT2, Slit homolog 2 protein, Slit-2

4 Images

Sandwich ELISA - Human Slit2 ELISA Kit (AB283885)

sELISA

Supplier Data

Sandwich ELISA - Human Slit2 ELISA Kit (AB283885)

Example of human Slit2 standard curve in Sample Diluent NS

sELISA

Supplier Data

Sandwich ELISA - Human Slit2 ELISA Kit (AB283885)

Interpolated concentrations of native Slit2 in human serum, plasma and cell culture supernatant samples.

The concentrations of Slit2 were measured in duplicates, interpolated from the Slit2 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma (citrate) 50%, plasma (EDTA) 50%, plasma (heparin) 25% and PC-3 supernatant 20%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Slit2 concentration was determined to be 349.7 pg/mL in serum, 378.5 pg/mL in plasma (citrate), 261.1 pg/mL in plasma (EDTA), 255.3 in plasma (heparin), and 5,175 pg/mL in PC-3 supernatant.

sELISA

Supplier Data

Sandwich ELISA - Human Slit2 ELISA Kit (AB283885)

Serum from ten individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Slit2 concentration was determined to be 212.2 pg/mL with a range of 98.33 – 381.3 pg/mL. Slit2 was undetected in one donor.

sELISA

Supplier Data

Sandwich ELISA - Human Slit2 ELISA Kit (AB283885)

Interpolated concentrations of native Slit2 in human PC-3 cell extract sample based on a 200 μg/mL extract load.

The concentrations of Slit2 were measured in duplicate and interpolated from the Slit2 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Slit2 concentration was determined to be 1,113 pg/mL in PC-3 cell extract.

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, Cell Lysate, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 16.9 pg/mL

Range

46.88 - 3000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human Slit2 ELISA Kit (ab283885) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Slit2 protein in serum, plasma, cell culture supernatant and cell and tissue extract. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Slit2 with 16.9 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Slit2, encoded by the SLIT2 gene, is a secreted extracellular matrix glycoprotein. Acts as molecular guidance cue in cellular migration that is classically studied for role in neuronal guidance during development. Slit2 function appear to be mediated by interactions with roundabout homolog receptor and participates in other cellular processes such as proliferation. Studies have shown Slit2 to be involved with angiogenesis, inflammation, and cancer.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Supernatant", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.4" }, { "reproducibilityType": "Intra", "sample": "Supernatant", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.8" } ]

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Recovery

[ { "sample": "Cell culture supernatant", "range": "98 - 109 %", "average": "= 105" }, { "sample": "Serum", "range": "80 - 82 %", "average": "= 81" }, { "sample": "EDTA Plasma", "range": "82 - 86 %", "average": "= 84" }, { "sample": "Heparin Plasma", "range": "85 - 92 %", "average": "= 89" }, { "sample": "Cell Lysate", "range": "90 - 105 %", "average": "= 98" }, { "sample": "Citrate plasma", "range": "83 - 85 %", "average": "= 85" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Slit2 also known as Slit homolog 2 protein is a guidance cue protein with a mass of approximately 200 kDa. It plays a role in axonal guidance and cellular migration. Slit2 is expressed in the central nervous system where it contributes to neural development. It is also found in various tissues such as the liver lungs and kidneys. Slit2 mediates its effects primarily through interaction with Roundabout (Robo) receptors.

Biological function summary

Slit2 functions in guiding axons during neuronal development and preventing them from straying from their pathways. It complexes with Robo receptors to transmit repulsive directional signals. This interaction is essential in the development of the nervous system ensuring proper neural circuit formation and synaptic connectivity. Besides neural guidance Slit2 also regulates other cellular events like angiogenesis and immune cell migration.

Pathways

Slit2 participates in the axon guidance pathway where it influences neural network structuring by interacting with Robo receptors and other guidance cues. The Slit-Robo pathway plays an important role in axonal repulsion working alongside the Netrin signaling pathway that involves proteins like DCC and UNC5. This way Slit2 and its pathways balance attractive and repulsive cues to form functional neuronal networks.

Slit2 has associations with cancers particularly glioblastoma where it affects tumor cell migration and invasion. Dysregulation of the Slit-Robo signaling may lead to enhanced cancer cell metastasis. Additionally Slit2 is linked to congenital heart diseases where its malfunctioning affects cardiac development. Its interaction with Robo and other proteins like Ephrin can influence these disease processes suggesting a broader role in both developmental and pathological contexts.

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Product protocols

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Target data

Thought to act as molecular guidance cue in cellular migration, and function appears to be mediated by interaction with roundabout homolog receptors. During neural development involved in axonal navigation at the ventral midline of the neural tube and projection of axons to different regions. SLIT1 and SLIT2 seem to be essential for midline guidance in the forebrain by acting as repulsive signal preventing inappropriate midline crossing by axons projecting from the olfactory bulb. In spinal cord development may play a role in guiding commissural axons once they reached the floor plate by modulating the response to netrin. In vitro, silences the attractive effect of NTN1 but not its growth-stimulatory effect and silencing requires the formation of a ROBO1-DCC complex. May be implicated in spinal cord midline post-crossing axon repulsion. In vitro, only commissural axons that crossed the midline responded to SLIT2. In the developing visual system appears to function as repellent for retinal ganglion axons by providing a repulsion that directs these axons along their appropriate paths prior to, and after passage through, the optic chiasm. In vitro, collapses and repels retinal ganglion cell growth cones. Seems to play a role in branching and arborization of CNS sensory axons, and in neuronal cell migration. In vitro, Slit homolog 2 protein N-product, but not Slit homolog 2 protein C-product, repels olfactory bulb (OB) but not dorsal root ganglia (DRG) axons, induces OB growth cones collapse and induces branching of DRG axons. Seems to be involved in regulating leukocyte migration.

See full target information SLIT2

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human Slit2 ELISA Kit

Sandwich ELISA - Human Slit2 ELISA Kit (AB283885)

Sandwich ELISA - Human Slit2 ELISA Kit (AB283885)

Sandwich ELISA - Human Slit2 ELISA Kit (AB283885)

Sandwich ELISA - Human Slit2 ELISA Kit (AB283885)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

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Primary Antibodies

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Product promise