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Human SLPI ELISA Kit (ALP) is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human SLPI (ALP) in Urine, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma samples.

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Key facts

Detection method

Colorimetric

Sample types

Urine, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

31.25 - 2000 pg/mL

Assay time

1h 30m

Sensitivity

= 5.59 pg/mL

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Reactivity data

Application

sELISA

Reactivity

Reacts

Dilution info

-

Notes

-

Target data

Function

Acid-stable proteinase inhibitor with strong affinities for trypsin, chymotrypsin, elastase, and cathepsin G (PubMed:3533531, PubMed:3462719, PubMed:2039600, PubMed:2110563, PubMed:10702419, PubMed:24121345). Modulates the inflammatory and immune responses after bacterial infection, and after infection by the intracellular parasite L.major. Down-regulates responses to bacterial lipopolysaccharide (LPS) (By similarity). Plays a role in regulating the activation of NF-kappa-B and inflammatory responses (PubMed:10702419, PubMed:24352879). Has antimicrobial activity against mycobacteria, but not against salmonella. Contributes to normal resistance against infection by M.tuberculosis. Required for normal resistance to infection by L.major. Required for normal wound healing, probably by preventing tissue damage by limiting protease activity (By similarity). Together with ELANE, required for normal differentiation and proliferation of bone marrow myeloid cells (PubMed:24352879).

Alternative names

What's included?

1 x 96 Tests
Components
10X Human SLPI (ALP) Capture Antibody
1 x 600 µL
10X Human SLPI (ALP) Detector Antibody
1 x 600 µL
10X Wash Buffer PT (ab206977)
1 x 20 mL
Antibody Diluent 4BI
1 x 6 mL
Human SLPI (ALP) Lyophilized Recombinant Protein
2 x 1 Vial
Plate Seals
1 x 1 Unit
Sample Diluent NS (ab193972)
2 x 50 mL
SimpleStep Pre-Coated 96-Well Microplate (ab206978)
1 x 1 Unit
Stop Solution
1 x 12 mL
TMB Development Solution
1 x 12 mL

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Human SLPI ELISA Kit (ALP) is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human SLPI (ALP) in Urine, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma samples.

Alternative names

Key facts

Detection method

Colorimetric

Sample types

Urine, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

31.25 - 2000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

Sensitivity

= 5.59 pg/mL

Precision

Intra assay

Sample

Serum

n

8

mean

-

SD

-

C.V.

6.1

Inter assay

Sample

Serum

n

3

mean

-

SD

-

C.V.

3.6

Recovery

Sample specific recovery

Sample type

Serum

Average %

= 117

Range

109 - 123 %

Sample type

EDTA Plasma

Average %

= 119

Range

98 - 151 %

Sample type

Urine

Average %

= 106

Range

99 - 109 %

Sample type

Heparin Plasma

Average %

= 105

Range

96 - 120 %

Sample type

Citrate plasma

Average %

= 113

Range

108 - 119 %

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

Human SLPI ELISA Kit (ALP) (ab263890) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of SLPI (ALP) protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human SLPI (ALP) with 5.59 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

SLPI (Secretory Leukocyte Protease Inhibitor), also known as ALP (Antileukoproteinase) is acid-stable proteinase inhibitor with strong affinities for trypsin, chymotrypsin, elastase, and cathepsin G. It modulates the inflammatory and immune responses after bacterial and parasite infection.

Supplementary info

Biological function summary

SLPI serves as a protector of epithelial surfaces from proteolytic degradation and acts as an anti-inflammatory agent. It displays broad-spectrum antimicrobial properties providing protection against bacterial and viral infections. Although SLPI is not part of a complex itself its interactions with extracellular matrix proteins help modulate wound healing processes and maintain tissue integrity.

Activity summary

Secretory leukocyte protease inhibitor (SLPI) also known as antileukoproteinase and neutrophil elastase inhibitor is a protein with a molecular mass of approximately 12 kDa. SLPI is found mainly in epithelial tissues and is expressed in locations such as the respiratory tract skin and cervix. Mechanically SLPI functions as a serine protease inhibitor binding to and neutralizing enzymes like neutrophil elastase cathepsin G and trypsin which helps to limit tissue damage during inflammation.

Pathways

SLPI participates in the innate immune response and inflammation regulation pathways. It interacts within pathways that involve the complement cascade and kallikrein-kinin system helping to regulate inflammation and modulate immune responses. During these processes SLPI is related to proteins such as alpha-1-antitrypsin in its role as a protease inhibitor contributing to the regulation of protease activity and inflammatory responses.

Associated diseases and disorders

SLPI is implicated in conditions such as chronic obstructive pulmonary disease (COPD) and cystic fibrosis where excessive protease activity leads to tissue damage. In these diseases SLPI shows a protective role against lung tissue degradation. SLPI is also associated with the progression of certain cancers like ovarian cancer where its overexpression may support tumor growth and metastasis. In these contexts SLPI relates to other protease inhibitors such as tissue inhibitors of metalloproteinases (TIMPs) due to their shared function in regulating protease activity and tissue remodeling.

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4 product images

  • Sandwich ELISA - Human SLPI ELISA Kit (ALP) (ab263890), expandable thumbnail

    Sandwich ELISA - Human SLPI ELISA Kit (ALP) (ab263890)

    Human SLPI standard curve comparison.

    Standard Curve comparison between human SLPI SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

  • Sandwich ELISA - Human SLPI ELISA Kit (ALP) (ab263890), expandable thumbnail

    Sandwich ELISA - Human SLPI ELISA Kit (ALP) (ab263890)

    The SLPI standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

  • Sandwich ELISA - Human SLPI ELISA Kit (ALP) (ab263890), expandable thumbnail

    Sandwich ELISA - Human SLPI ELISA Kit (ALP) (ab263890)

    Interpolated concentrations of native SLPI in human serum, plasma and urine samples.

    Undiluted samples are as follows: serum 3%, plasma (citrate) 3%, plasma (EDTA) 3%, plasma (heparin) 3% and urine 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean SLPI concentration was determined to be 55.8 ng/mL in neat serum, 40.9 ng/mL in neat plasma (citrate), 46.4 ng/mL in neat plasma (EDTA), 31.7 ng/mL in neat plasma (heparin) and 2.43 ng/mL in neat urine.

  • Sandwich ELISA - Human SLPI ELISA Kit (ALP) (ab263890), expandable thumbnail

    Sandwich ELISA - Human SLPI ELISA Kit (ALP) (ab263890)

    Serum from ten individual healthy human male donors was measured in duplicate.

    Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean SLPI concentration was determined to be 62.6 ng/mL with a range of 41.2 – 85 ng/mL.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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