JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB263890

Human SLPI ELISA Kit (ALP)

Be the first to review this product! Submit a review

|

(2 Publications)

Human SLPI ELISA Kit (ALP) is a single-wash 90-min Simplestep used to quantify Human SLPI (ALP) with a sensitivity of 5.59 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

WAP4, WFDC4, SLPI, Antileukoproteinase, ALP, BLPI, HUSI-1, Mucus proteinase inhibitor, Protease inhibitor WAP4, Secretory leukocyte protease inhibitor, Seminal proteinase inhibitor, WAP four-disulfide core domain protein 4, MPI

4 Images
Sandwich ELISA - Human SLPI ELISA Kit (ALP) (AB263890)
  • sELISA

Supplier Data

Sandwich ELISA - Human SLPI ELISA Kit (ALP) (AB263890)

The SLPI standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human SLPI ELISA Kit (ALP) (AB263890)
  • sELISA

Supplier Data

Sandwich ELISA - Human SLPI ELISA Kit (ALP) (AB263890)

Serum from ten individual healthy human male donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean SLPI concentration was determined to be 62.6 ng/mL with a range of 41.2 – 85 ng/mL.

Sandwich ELISA - Human SLPI ELISA Kit (ALP) (AB263890)
  • sELISA

Supplier Data

Sandwich ELISA - Human SLPI ELISA Kit (ALP) (AB263890)

Interpolated concentrations of native SLPI in human serum, plasma and urine samples.

Undiluted samples are as follows : serum 3%, plasma (citrate) 3%, plasma (EDTA) 3%, plasma (heparin) 3% and urine 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean SLPI concentration was determined to be 55.8 ng/mL in neat serum, 40.9 ng/mL in neat plasma (citrate), 46.4 ng/mL in neat plasma (EDTA), 31.7 ng/mL in neat plasma (heparin) and 2.43 ng/mL in neat urine.

Sandwich ELISA - Human SLPI ELISA Kit (ALP) (AB263890)
  • sELISA

Supplier Data

Sandwich ELISA - Human SLPI ELISA Kit (ALP) (AB263890)

Human SLPI standard curve comparison.

Standard Curve comparison between human SLPI SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

Key facts

Detection method

Colorimetric

Sample types

Urine, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 5.59 pg/mL

Range

31.25 - 2000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style
left-column

Complementary Products

Assay Kits

AB270204

Human Neutrophil Elastase ELISA Kit

Recombinant|SimpleStep

ELISA - Human Neutrophil Elastase ELISA Kit (AB270204)

  • 0
  • 0
Assay Kits

AB254503

Human Alkaline Phosphatase ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human Alkaline Phosphatase ELISA Kit (AB254503)

  • 0
  • 0
Assay Kits

AB270202

Human Osteocalcin ELISA Kit

Recombinant|SimpleStep

ELISA - Human Osteocalcin ELISA Kit (AB270202)

  • 5
  • 1
Assay Kits

AB272203

Human TGFB3 ELISA Kit

Recombinant|SimpleStep

ELISA - Human TGFB3 ELISA Kit (AB272203)

  • 5
  • 1
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Human SLPI ELISA Kit (ALP) (ab263890) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of SLPI (ALP) protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human SLPI (ALP) with 5.59 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

SLPI (Secretory Leukocyte Protease Inhibitor), also known as ALP (Antileukoproteinase) is acid-stable proteinase inhibitor with strong affinities for trypsin, chymotrypsin, elastase, and cathepsin G. It modulates the inflammatory and immune responses after bacterial and parasite infection.

style
left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.6" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.1" } ]
style
left-column

Recovery

[ { "sample": "Serum", "range": "109 - 123 %", "average": "= 117" }, { "sample": "EDTA Plasma", "range": "98 - 151 %", "average": "= 119" }, { "sample": "Urine", "range": "99 - 109 %", "average": "= 106" }, { "sample": "Heparin Plasma", "range": "96 - 120 %", "average": "= 105" }, { "sample": "Citrate plasma", "range": "108 - 119 %", "average": "= 113" } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab263890-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB263890-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB263890-CMP05", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB263890-CMP10", "productcode":"" }, { "size":"2 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB263890-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB263890-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB263890-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human SLPI (ALP) Lyophilized Recombinant Protein", "number":"AB263890-CMP07", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB263890-CMP08", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human SLPI (ALP) Capture Antibody", "number":"AB263890-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human SLPI (ALP) Detector Antibody", "number":"AB263890-CMP02", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Secretory leukocyte protease inhibitor (SLPI) also known as antileukoproteinase and neutrophil elastase inhibitor is a protein with a molecular mass of approximately 12 kDa. SLPI is found mainly in epithelial tissues and is expressed in locations such as the respiratory tract skin and cervix. Mechanically SLPI functions as a serine protease inhibitor binding to and neutralizing enzymes like neutrophil elastase cathepsin G and trypsin which helps to limit tissue damage during inflammation.
Biological function summary

SLPI serves as a protector of epithelial surfaces from proteolytic degradation and acts as an anti-inflammatory agent. It displays broad-spectrum antimicrobial properties providing protection against bacterial and viral infections. Although SLPI is not part of a complex itself its interactions with extracellular matrix proteins help modulate wound healing processes and maintain tissue integrity.

Pathways

SLPI participates in the innate immune response and inflammation regulation pathways. It interacts within pathways that involve the complement cascade and kallikrein-kinin system helping to regulate inflammation and modulate immune responses. During these processes SLPI is related to proteins such as alpha-1-antitrypsin in its role as a protease inhibitor contributing to the regulation of protease activity and inflammatory responses.

SLPI is implicated in conditions such as chronic obstructive pulmonary disease (COPD) and cystic fibrosis where excessive protease activity leads to tissue damage. In these diseases SLPI shows a protective role against lung tissue degradation. SLPI is also associated with the progression of certain cancers like ovarian cancer where its overexpression may support tumor growth and metastasis. In these contexts SLPI relates to other protease inhibitors such as tissue inhibitors of metalloproteinases (TIMPs) due to their shared function in regulating protease activity and tissue remodeling.
style
left-column

Product protocols

style
left-column

Target data

Acid-stable proteinase inhibitor with strong affinities for trypsin, chymotrypsin, elastase, and cathepsin G (PubMed : 10702419, PubMed : 2039600, PubMed : 2110563, PubMed : 24121345, PubMed : 3462719, PubMed : 3533531). Modulates the inflammatory and immune responses after bacterial infection, and after infection by the intracellular parasite L.major. Down-regulates responses to bacterial lipopolysaccharide (LPS) (By similarity). Plays a role in regulating the activation of NF-kappa-B and inflammatory responses (PubMed : 10702419, PubMed : 24352879). Has antimicrobial activity against mycobacteria, but not against salmonella. Contributes to normal resistance against infection by M.tuberculosis. Required for normal resistance to infection by L.major. Required for normal wound healing, probably by preventing tissue damage by limiting protease activity (By similarity). Together with ELANE, required for normal differentiation and proliferation of bone marrow myeloid cells (PubMed : 24352879).
See full target information SLPI
style
left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 13:23013 PubMed38155270

2023

Recombinant human secretory leukocyte protease inhibitor (rhSLPI) coated titanium enhanced human osteoblast adhesion and differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Radchanon Leelasukseree,Wannapat Chouyratchakarn,Chayanisa Phutiyothin,Faprathan Pikwong,Onnicha Srisopar,Phornsawat Baipaywad,Suruk Udomsom,Podsawee Mongkolpathumrat,Chayarop Supanchart,Sarawut Kumphune

Journal of immunology research 2021:1057186 PubMed33506054

2021

Reduced Expression of Antimicrobial Protein Secretory Leukoprotease Inhibitor and Clusterin in Chronic Rhinosinusitis with Nasal Polyps.

Applications

Unspecified application

Species

Unspecified reactive species

Yanran Huang,Ming Wang,Yu Hong,Xiangting Bu,Ge Luan,Yang Wang,Ying Li,Hongfei Lou,Chengshuo Wang,Luo Zhang
View all publications
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com