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AB155439

Human SMAC ELISA Kit

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Human SMAC ELISA Kit is a Sandwich ELISA for the measurement of Human SMAC in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

SMAC, DIABLO, Diablo IAP-binding mitochondrial protein, Direct IAP-binding protein with low pI, Second mitochondria-derived activator of caspases

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ELISA - Human SMAC ELISA Kit (AB155439)
  • ELISA

Supplier Data

ELISA - Human SMAC ELISA Kit (AB155439)

SMAC ELISA Standard Curve.

Representative Standard Curve using ab155439.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

< 0.1 ng/mL

Range

0.12 - 30 ng/mL

Assay Platform

Microplate

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's SMAC Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay designed for the quantitative measurement of Human SMAC in serum, plasma (Human SMAC concentration is pretty low in normal serum/plasma. It may not be in this assay) and cell culture supernatants.

This assay employs an antibody specific for Human SMAC coated on a 96-well plate. Standards and samples are pipetted into the wells and SMAC present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human SMAC antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of SMAC bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Optimization may be required with urine samples.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" } ]
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Recovery

[ { "sample": "Serum", "range": "95 - 101 %", "average": "= 103.3" }, { "sample": "Plasma", "range": "86 - 116 %", "average": "= 97.55" }, { "sample": "Cell culture media", "range": "96 - 114 %", "average": "= 104.8" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab155439-1x96Tests", "assetComponentDetails": [ { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB155439-CMP03", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB155439-CMP01", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB155439-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SMAC Microplate (12 x 8 wells)", "number":"AB155439-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB155439-CMP08", "productcode":"" }, { "size":"1 x 200 µL", "name":"400X HRP-Streptavidin Concentrate", "number":"AB155439-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Human SMAC Standard (lyophilized)", "number":"AB155439-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Human SMAC", "number":"AB155439-CMP07", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Smac/Diablo is a mitochondrial protein that promotes apoptosis by interfering with inhibitor of apoptosis proteins (IAPs). The protein also known as Second Mitochondria-derived Activator of Caspases weighs approximately 27 kDa. Researchers detect Smac/Diablo in various tissues with higher expression levels in organs like the heart and brain. This protein's expression also varies depending on the cell's state and external stimuli.
Biological function summary

Smac/Diablo plays a significant role in programmed cell death by binding to IAPs and negating their inhibition of caspases the key executors of apoptosis. Smac/Diablo releases from mitochondria into the cytosol when apoptotic signals activate. It does not form complexes but interacts with IAPs facilitating the activation of caspases and enhancing the apoptotic response. Its interaction with IAPs highlights its important function in apoptosis regulation.

Pathways

Smac/Diablo integrates into the mitochondrial apoptosis pathway contributing to the intrinsic pathway of apoptosis. It closely interacts with proteins such as caspases and IAPs. This integration is important for apoptosis regulation linking mitochondrial outer membrane permeabilization with the activation of caspases. Additionally the protein engages in the cytochrome c pathway promoting apoptosis by restricting IAPs and aiding in the release of cytochrome c important for apoptosis progression.

Researchers associate Smac/Diablo with cancer and neurodegenerative diseases like Alzheimer's disease. In cancer elevated Smac/Diablo levels can result in increased apoptotic death of cancerous cells potentially serving as a target for therapy. It also interacts with proteins like XIAP in these disease contexts. In neurodegenerative disorders dysregulation of apoptosis pathways involving Smac/Diablo might contribute to excessive neuronal cell death. Understanding Smac/Diablo's role in these diseases provides insights into possible therapeutic interventions.
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Product protocols

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Target data

Promotes apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway. Acts by opposing the inhibitory activity of inhibitor of apoptosis proteins (IAP). Inhibits the activity of BIRC6/BRUCE by inhibiting its binding to caspases (PubMed : 15200957, PubMed : 36758104, PubMed : 36758105, PubMed : 36758106).. Isoform 3. Attenuates the stability and apoptosis-inhibiting activity of XIAP/BIRC4 by promoting XIAP/BIRC4 ubiquitination and degradation through the ubiquitin-proteasome pathway. Also disrupts XIAP/BIRC4 interacting with processed caspase-9 and promotes caspase-3 activation.. Isoform 1. Defective in the capacity to down-regulate the XIAP/BIRC4 abundance.
See full target information DIABLO
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websiteProtocolBooklet
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Product promise

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