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AB264624

Human SMAD3 ELISA Kit

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(1 Publication)

Human SMAD3 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human SMAD3 with a sensitivity of 55.72 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

MADH3, SMAD3, Mothers against decapentaplegic homolog 3, MAD homolog 3, Mad3, Mothers against DPP homolog 3, hMAD-3, JV15-2, SMAD family member 3, SMAD 3, Smad3, hSMAD3

3 Images
Sandwich ELISA - Human SMAD3 ELISA Kit (AB264624)
  • sELISA

Supplier Data

Sandwich ELISA - Human SMAD3 ELISA Kit (AB264624)

Example of SMAD3 standard curve in 1X Cell Extraction Buffer PTR.

The SMAD3 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human SMAD3 ELISA Kit (AB264624)
  • sELISA

Supplier Data

Sandwich ELISA - Human SMAD3 ELISA Kit (AB264624)

Interpolated concentrations of native SMAD3 in A431, A549, NIH3T3, and C6 cell extract samples based on a 250 μg/mL, 250 μg/mL, 250 μg/mL, and 125 μg/mL extract loads, respectively.

The concentrations of SMAD3 were measured in duplicate and interpolated from the SMAD3 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean SMAD3 concentration was determined to be 6.6 ng/mL in A431 cell extract samples, 5 ng/mL in A549 cell extract samples, 5.2 ng/mL in NIH3T3 cell extract samples, and 3.3 ng/mL in C6 cell extract samples.

Sandwich ELISA - Human SMAD3 ELISA Kit (AB264624)
  • sELISA

Supplier Data

Sandwich ELISA - Human SMAD3 ELISA Kit (AB264624)

Representative image of Human SMAD3 ELISA Kit (ab264624)

Components shown from left to right :

- 10X Wash Buffer PT (ab206977)

- 10X Human SMAD3 ELISA Kit Capture Antibody

- 10X Human SMAD3 Detector Antibody

- 10X Human SMAD3 ELISA Kit Lyophilised Purified Protein (2 vials)

- Human SMAD3 ELISA Kit 50X Cell Extraction Enhancer Solution (ab193971)

- Sample diluent NS

- TMB solution

- Stop Solution

- 5X Cell Extraction Buffer PTR (ab193970)

- Antibody Diluent 4BI

SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.

Plate Seals - shown underneath bottles / vials.

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 55.72 pg/mL

Range

187.5 - 12000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human SMAD3 ELISA Kit (ab264624) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of SMAD3 protein in cell lysate. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human SMAD3 with 55.72 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

SMAD3 is a key protein involved in cytokine signaling and is essential for the proper regulation of proliferation, differentiation and apoptosis. Upon phosphorylation by TGF-b receptors, SMAD3 complexes with SMAD4 and upregulates goes controlled by SMAD-binding elements. SMAD3 is currently under investigation for its role in type 2 diabetes and cancer. The immunogen for this kit is an N-terminal fragment and shares high homology with many mammalian homologs.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1.5" }, { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1.2" } ]
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Recovery

[ { "sample": "Cell Lysate", "range": "107 - 112 %", "average": "= 109" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab264624-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB264624-CMP04", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB264624-CMP12", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB264624-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB264624-CMP13", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB264624-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB264624-CMP09", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X SMAD3 Capture Antibody", "number":"AB264624-CMP10", "productcode":"" }, { "size":"2 x 1 Vial", "name":"SMAD3 Lyophilized Recombinant Protein", "number":"AB264624-CMP03", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB264624-CMP08", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X SMAD3 Detector Antibody", "number":"AB264624-CMP11", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB264624-CMP02", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB264624-CMP07", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Smad3 also known as Mothers against decapentaplegic homolog 3 is a protein that plays a mechanical role in signal transduction. It acts mainly as a transcription factor and gets activated through phosphorylation. The molecular weight of Smad3 is approximately 48 kDa. It is expressed widely across numerous tissues including the cellular nucleus where it executes its function after activation.
Biological function summary

Smad3 acts as a mediator of signal transduction for the TGF-beta (transforming growth factor-beta) superfamily forming a complex with phosphorylated Smad2. This enables it to regulate transcriptional activity influencing cell proliferation differentiation and apoptosis. Smad3 also participates in various cellular processes by interacting with other co-factors and regulatory proteins that aid in fine-tuning its function.

Pathways

Smad3 plays an important role in the TGF-beta signaling pathway where it works closely with Smad4 to propagate the signal. Upon phosphorylation it forms a complex with co-Smad (Smad4) and moves into the nucleus to influence gene expression. Smad3 is also involved in pathways related to oncogenesis and tissue fibrosis indicating its significant role in cellular regulation and response mechanisms.

Smad3 is associated with fibrotic diseases and cancers particularly in tissues such as the liver and lungs. Altered Smad3 signaling contributes to the pathological process occurring in fibrotic disorders often interacting with Smad4 in these abnormalities. Dysregulated Smad3 expression or mutations can also lead to oncogenic transformations highlighting its critical involvement in disease states.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

SMAD3 is a receptor-regulated SMAD that functions as an intracellular signal transducer and transcriptional modulator, activated by TGF-beta and activin type 1 receptor kinases. It binds to the TRE element in promoters of numerous genes regulated by TGF-beta, and upon forming a complex with SMAD4, activates transcription. Additionally, SMAD3 can form a complex with SMAD4, JUN, and FOS at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. SMAD3 may inhibit wound healing by modulating the growth and migration of primary keratinocytes and altering TGF-beta-mediated monocyte chemotaxis, with this effect being potentially hormone-sensitive. Furthermore, SMAD3 is involved in regulating chondrogenesis and osteogenesis and may inhibit early bone fracture healing. It also positively regulates PDPK1 kinase activity by promoting its dissociation from the 14-3-3 protein YWHAQ, which negatively regulates it. This supplementary information is collated from multiple sources and compiled automatically.
See full target information SMAD3
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Toxics 11: PubMed38133427

2023

TGF-β Regulates mA RNA Methylation after PM Exposure.

Applications

Unspecified application

Species

Unspecified reactive species

Tingting Wu,Bingqian Liu,Yongjie Wei,Zhigang Li
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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