AB315434

Human SMOC1 ELISA Kit

SimpleStep
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Human SMOC1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human SMOC1 with a sensitivity of 20.183 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

SPARC-related modular calcium-binding protein 1, Secreted modular calcium-binding protein 1, SMOC-1, SMOC1

2 Images

Sandwich ELISA - Human SMOC1 ELISA Kit (AB315434)

sELISA

Supplier Data

Sandwich ELISA - Human SMOC1 ELISA Kit (AB315434)

Interpolated concentrations of Human SMOC1 in HepG2 cell extract and human testis tissue extract

Interpolated concentration of native SMOC1 was measured in duplicate at different sample concentrations. Undiluted samples are as follows : HepG2 cell extract 500 µg/mL and human testis tissue extract 250 µg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

sELISA

Supplier Data

Sandwich ELISA - Human SMOC1 ELISA Kit (AB315434)

Example of Human SMOC1 standard curve in 1X Cell Extraction Buffer PTR. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate, Tissue Extracts

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 20.183 pg/mL

Range

62.5 - 4000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human SMOC1 SimpleStep ELISA^® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of SMOC1 protein in Cell Lysate, Tissue extracts. Quantitate Human SMOC1 with 20.183 pg/ml sensitivity.

SimpleStep ELISA^® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA^® protocol summary in the image section for further details. Our SimpleStep ELISA^® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA^® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA^® kits.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.7" }, { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1.3" } ]

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Recovery

[ { "sample": "Cell Lysate", "range": "108 - 115 %", "average": "= 111" }, { "sample": "Tissue Extracts", "range": "96 - 104 %", "average": "= 101" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab315434-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB315434-CMP10", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB315434-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB315434-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB315434-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB315434-CMP08", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPR2", "number":"AB315434-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human SMOC1 Lyophilized Recombinant Protein", "number":"AB315434-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human SMOC1 Capture Antibody", "number":"AB315434-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB315434-CMP11", "productcode":"" }, { "size":"1 x 600 µL", "name":"Human SMOC1 Detector Antibody", "number":"AB315434-CMP02", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB315434-CMP05", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SPARC-related modular calcium binding 1 (SMOC1) is an extracellular matrix protein coded by the SMOC1 gene. Known also as its full form the protein weighs approximately 59 kDa and consists of several domains including a thyroglobulin type-1 repeat and two EF-hand domains. SMOC1 is widely expressed in various tissues such as the eye bone and skin. Its expression pattern suggests it plays an important role in tissue development and maintenance.

Biological function summary

SMOC1 functions involve regulation of angiogenesis and cellular differentiation. It interacts with the extracellular matrix modulating the assembly and dynamics of structural proteins. SMOC1 sounds interactions with calmodulin and integrins forming part of a larger complex. This complex engagement influences cellular processes like cell migration adhesion and stabilization which are pivotal for development and wound healing.

Pathways

SMOC1 associates with integrin signaling and TGF-beta signaling pathways. These pathways govern processes like cell growth apoptosis and differentiation. SMOC1 modulates the activity of integrins connecting with proteins such as integrin alpha-V beta-3 and transforming growth factor beta (TGF-β). Their relationships indicate SMOC1's involvement in cellular communication and response to environmental changes.

SMOC1 links to conditions like microphthalmia and coloboma as well as bone development disorders. Mutations or dysregulation of SMOC1 impairs normal development indicating its role in ocular and skeletal health. Researchers note connections with proteins like Pax6 which is also involved in eye development. This involvement shows how closely SMOC1 is tied to disorders affecting vision and structural integrity.

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Product protocols

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Target data

Plays essential roles in both eye and limb development. Probable regulator of osteoblast differentiation.

See full target information SPARC-related modular calcium-binding protein 1

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human SMOC1 ELISA Kit

Sandwich ELISA - Human SMOC1 ELISA Kit (AB315434)

Sandwich ELISA - Human SMOC1 ELISA Kit (AB315434)

Key facts

Detection method

Sample types

Reacts with

Assay type

Results type

Sensitivity

Range

Assay time

Assay Platform

Complementary Products

Assay Kits

Proteins & Peptides

Primary Antibodies

Assay Kits

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise