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AB314350

Human SMOC2 ELISA Kit

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Human SMOC2 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human SMOC2 in Human in Cell Culture Media, Biofluids samples.

View Alternative Names

SMAP2, MSTP117, SMOC2, SPARC-related modular calcium-binding protein 2, Secreted modular calcium-binding protein 2, Smooth muscle-associated protein 2, SMOC-2, SMAP-2

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Sandwich ELISA - Human SMOC2 ELISA Kit (AB314350)
  • sELISA

Supplier Data

Sandwich ELISA - Human SMOC2 ELISA Kit (AB314350)

Example of standard curve using ab314350. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Plasma, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 18 pg/mL

Range

18 - 4000 pg/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human SMOC2 ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human SMOC2 in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human SMOC2 coated on a 96-well plate.
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Recovery

[ { "sample": "Serum", "range": "70 - 85 %", "average": "= 77.26" }, { "sample": "Plasma", "range": "93 - 105 %", "average": "= 98.18" }, { "sample": "Cell culture media", "range": "101 - 108 %", "average": "= 103.9" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab314350-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB314350-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human SMOC2 Detection Antibody", "number":"AB314350-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human SMOC2 Antibody-coated ELISA Plate", "number":"AB314350-CMP08", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB314350-CMP04", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB314350-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human SMOC2 Protein Standard", "number":"AB314350-CMP10", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent (Item E2)", "number":"AB314350-CMP05", "productcode":"" }, { "size":"1 x 200 µL", "name":"150X HRP-Streptavidin", "number":"AB314350-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"ELISA Microplate Bag", "number":"AB314350-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SMOC2 also known as SPARC-related modular calcium-binding protein 2 functions mechanically as an extracellular matrix-associated protein. It weighs approximately 48 kDa. SMOC2 shows expression mainly in tissues like bone skin and blood vessels where it contributes to cellular processes through its interaction with other matrix components. This protein contains multiple functional domains that allow it to bind calcium ions and interact with various molecules within the extracellular matrix.
Biological function summary

SMOC2 influences several processes related to tissue development and remodeling. It is involved in angiogenesis and cell proliferation impacting cellular communication through the matrix. SMOC2 does not usually form part of any large structural complexes but interacts with extracellular matrix proteins such as collagen and fibronectin to modulate their effects on cell behavior. It can regulate interactions between cells and their surrounding environment thereby contributing to maintaining tissue homeostasis.

Pathways

SMOC2 participates in the TGF-beta signaling pathway and the integrin-mediated signaling pathway. In the TGF-beta signaling pathway SMOC2 affects the regulation of growth and differentiation in various cell types. It interacts with proteins such as TGF-beta and related receptors influencing their activity and downstream effects. While involved in integrin pathways SMOC2 further affects cell adhesion and migration by engaging with integrin receptors thereby playing a role in modulating cellular responses to environmental changes.

SMOC2 has associations with cancer progression and fibrosis. Abnormal SMOC2 expression can promote tumor growth as seen in some types of cancer due to its role in cell proliferation and angiogenesis. In the context of fibrosis SMOC2 might exacerbate tissue scarring processes by influencing extracellular matrix remodeling. SMOC2 also shows a connection with proteins like TGF-beta in these diseases highlighting its involvement in modifying pathological states through these signaling pathways.
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Product protocols

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Target data

Promotes matrix assembly and cell adhesiveness (By similarity). Can stimulate endothelial cell proliferation, migration, as well as angiogenesis.
See full target information SMOC2
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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