Human SMOC2 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human SMOC2 in Human in Cell culture supernatant, Plasma, Serum samples.
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture supernatant, Plasma, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 18 - 4000 pg/mL
- Sensitivity
- = 18 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Promotes matrix assembly and cell adhesiveness (By similarity). Can stimulate endothelial cell proliferation, migration, as well as angiogenesis.
Alternative names
SMAP2, MSTP117, SMOC2, SPARC-related modular calcium-binding protein 2, Secreted modular calcium-binding protein 2, Smooth muscle-associated protein 2, SMOC-2, SMAP-2
What's included?
Human SMOC2 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human SMOC2 in Human in Cell culture supernatant, Plasma, Serum samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture supernatant, Plasma, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 18 - 4000 pg/mL
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 18 pg/mL
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 77.26 | Range 70 - 85 % |
Sample type Plasma | Average % = 98.18 | Range 93 - 105 % |
Sample type Cell culture media | Average % = 103.9 | Range 101 - 108 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Human SMOC2 ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human SMOC2 in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human SMOC2 coated on a 96-well plate.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SMOC2 also known as SPARC-related modular calcium-binding protein 2 functions mechanically as an extracellular matrix-associated protein. It weighs approximately 48 kDa. SMOC2 shows expression mainly in tissues like bone skin and blood vessels where it contributes to cellular processes through its interaction with other matrix components. This protein contains multiple functional domains that allow it to bind calcium ions and interact with various molecules within the extracellular matrix.
- Biological function summary
SMOC2 influences several processes related to tissue development and remodeling. It is involved in angiogenesis and cell proliferation impacting cellular communication through the matrix. SMOC2 does not usually form part of any large structural complexes but interacts with extracellular matrix proteins such as collagen and fibronectin to modulate their effects on cell behavior. It can regulate interactions between cells and their surrounding environment thereby contributing to maintaining tissue homeostasis.
- Pathways
SMOC2 participates in the TGF-beta signaling pathway and the integrin-mediated signaling pathway. In the TGF-beta signaling pathway SMOC2 affects the regulation of growth and differentiation in various cell types. It interacts with proteins such as TGF-beta and related receptors influencing their activity and downstream effects. While involved in integrin pathways SMOC2 further affects cell adhesion and migration by engaging with integrin receptors thereby playing a role in modulating cellular responses to environmental changes.
- Associated diseases and disorders
SMOC2 has associations with cancer progression and fibrosis. Abnormal SMOC2 expression can promote tumor growth as seen in some types of cancer due to its role in cell proliferation and angiogenesis. In the context of fibrosis SMOC2 might exacerbate tissue scarring processes by influencing extracellular matrix remodeling. SMOC2 also shows a connection with proteins like TGF-beta in these diseases highlighting its involvement in modifying pathological states through these signaling pathways.
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1 product image
Sandwich ELISA - Human SMOC2 ELISA Kit (ab314350)
Example of standard curve using ab314350. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.
Downloads
Product protocols
- Visit the general protocols
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