Skip to main content

Human SNAIL ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human SNAIL in Cell culture extracts samples.

This product has no reviews yet! Submit a review
Detection method

Colorimetric

Sample types

Cell culture extracts

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

78.125 - 5000 pg/mL

Assay time

1h 30m

Sensitivity

= 25.385 pg/mL

Loading...
Loading...
Loading...
Loading...
Loading...
Loading...
Loading...
Loading...

Reactivity data

Application
sELISA
Reactivity
Reacts
Dilution info
-
Notes

-

Images

Target data

What's included?

1 x 96 Tests
Components
10X Human SNAIL Capture Antibody
1 x 600 µL
10X Human SNAIL Detector Antibody
1 x 600 µL
10X Wash Buffer PT (ab206977)
1 x 20 mL
5X Cell Extraction Buffer PTR (ab193970)
1 x 10 mL
Antibody Diluent CPR2
1 x 6 mL
Human SNAIL Lyophilized Recombinant Protein
2 x 1 Vial
Plate Seals
1 x 1 Unit
Sample Diluent NS (ab193972)
1 x 12 mL
SimpleStep Pre-Coated 96-Well Microplate (ab206978)
1 x 1 Unit
Stop Solution
1 x 12 mL
TMB Development Solution
1 x 12 mL

Recommended products

  1. Loading...
  2. Loading...
  3. Loading...
  4. Loading...

Human SNAIL ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human SNAIL in Cell culture extracts samples.

Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

78.125 - 5000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

Sensitivity

= 25.385 pg/mL

General recovery

80%

Precision

Intra assay

Sample
Extract
n
8
mean
-
SD
-
C.V.
3.4

Inter assay

Sample
Extract
n
3
mean
-
SD
-
C.V.
2.8

Recovery

Sample specific recovery

Sample type
Cell culture extracts
Average %
= 108
Range
103 - 114 %

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

Human SNAIL SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of SNAIL protein in Cell culture extracts. Quantitate Human SNAIL with 25.385 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

        -Single-wash protocol reduces assay time to 90 minutes or less
        -High sensitivity, specificity and reproducibility from superior antibodies
        -Fully validated in biological samples
        -96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

There was a problem

We can’t download that datasheet. Please try again. If you need help, contact our Customer Services team at technical@abcam.com