AB178012

Human SOD2 ELISA Kit

SimpleStep
What is this?

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(6 Publications)

Human SOD2 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human SOD2 with a sensitivity of 0.22 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Cited in over 5 citations

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6 Images

sELISA

Supplier Data

Sandwich ELISA - Human SOD2 ELISA Kit (AB178012)

Example of SOD2 standard curve.

Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human SOD2 ELISA Kit (AB178012)

Titration of HepG2 cell lysate within the working range of the assay.

Background-subtracted data values from triplicate measurements (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human SOD2 ELISA Kit (AB178012)

Representative image of Human SOD2 ELISA Kit (ab178012)

Components shown from left to right :

- 10X Wash Buffer PT (ab206977)

- 10X Human SOD2 Capture Antibody

- 10X Human SOD2 Detector Antibody

- Human SOD2 Lyophilised Purified Protein (2 vials)

- 50X Cell Extraction Enhancer Solution (ab193971)

- Sample diluent NS

- TMB solution

- Stop Solution

- 5X Cell Extraction Buffer PTR (ab193970)

- Antibody diluent 5B

SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.

Plate Seals - shown underneath bottles / vials.

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit

sELISA

Supplier Data

Sandwich ELISA - Human SOD2 ELISA Kit (AB178012)

Quantification of SOD2 expression in various cell lines (HepG2, HeLa, Jurkat, MDA-MB-453 (MDA453) and SH-SY5Y (SH-5Y)) using SOD2 Human SimpleStep ELISA Kit^TM.

sELISA

Supplier Data

Sandwich ELISA - Human SOD2 ELISA Kit (AB178012)

Comparison of SOD2 expression in various cell lines (HepG2, HeLa, Jurkat, MDA-MB-453 (MDA453) and SH-SY5Y (SH-5Y)) using SOD2 Human SimpleStep™ ELISA Kit.

Background-subtracted data values from triplicate measurements of two lysate concentrations (3 and 1.5 μg/mL) are graphed as mean +/- SD.

Supplier Data

Western blot - Human SOD2 ELISA Kit (AB178012)

Comparison of SOD2 expression in various cell lines by Western blotting.

Cell lysates (20 μg) of HepG2 (lane 2), HeLa (lane 3), Jurkat (lane 4), MDA-MB-453 (lane 5) and SH-SY5Y (lane 6) were analyzed by Western blotting using ab13533 as primary antibody. Lane 1 shows migration of molecular weight marker.

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Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts, Tissue Extracts

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 0.22 ng/mL

Range

0.78 - 50 ng/mL

Assay time

1h 30m

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human SOD2 (Superoxide Dismutase 2) ELISA kit (ab178012) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of SOD2 protein in human cell and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate human SOD2 with 220 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpeStep ELISA® kits.

The principle cellular anti-oxidant enzymes are the superoxide dismutase family (SOD, E.C. 1.15.1.1). These enzymes dismutate superoxide into hydrogen peroxide which is further detoxified by other cellular defenses such as glutathione peroxidase and catalase. Superoxide and its products have been implicated in a wide range of diseases including cancer, inflammation, neurodegenerative diseases, diabetes and aging. The SOD family has 3 members, two of which are Cu-Zn type – the extracellular SOD3 and the cytoplasmic SOD1. The other member is the mitochondrial Mn (manganese) type SOD2. The mitochondrial Mn-SOD2 is a homotetramer of subunit mass 23 kDa in the mitochondrial matrix. SOD2 has been shown to be essential since knockout mice die shortly after birth. SOD2 levels may be downregulated in tumor cells and studies show that over expression of SOD2 in tumor cells may suppress cell division and cancer growth (Oberley, Biomedecine & Pharmacotherapy, 2005, 59, p143-8).

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "HepG2", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.2" }, { "reproducibilityType": "Intra", "sample": "HepG2", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.8" } ]

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Recovery

[ { "sample": "Cell culture media", "range": "85.5 - 95.6 %", "average": "= 92.1" }, { "sample": "Fetal Bovine Serum", "range": "75.7 - 82 %", "average": "= 79" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab178012-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB178012-CMP05", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB178012-CMP12", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB178012-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB178012-CMP13", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB178012-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB178012-CMP09", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5B", "number":"AB178012-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human SOD2 Capture Antibody", "number":"AB178012-CMP10", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human SOD2 Lyophilized Recombinant Protein", "number":"AB178012-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB178012-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human SOD2 Detector Antibody", "number":"AB178012-CMP11", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB178012-CMP08", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

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Target data

Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems.

See full target information Superoxide dismutase [Mn], mitochondrial

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Cancers 15: PubMed37835437

2023

Blockade of IL-10 Signaling Ensures Mifamurtide Efficacy in Metastatic Osteosarcoma.

Applications

Unspecified application

Species

Unspecified reactive species

Nicoletta Nastasi,Amada Pasha,Gennaro Bruno,Angela Subbiani,Laura Pietrovito,Angela Leo,Lucia Scala,Lorena de Simone,Gabriella Casazza,Federica Lunardi,Maria Letizia Taddei,Angela Tamburini,Annalisa Tondo,Claudio Favre,Maura Calvani

Scientific reports 13:4903 PubMed36966172

2023

The abundances of LTF and SOD2 in amniotic fluid are potential biomarkers of gestational age and preterm birth.

Applications

Unspecified application

Species

Unspecified reactive species

Te-Yao Hsu,Hsin-Hsin Cheng,Kuo-Chung Lan,Hsuan-Ning Hung,Yun-Ju Lai,Chih-Chang Tsai,Wen-Lang Fan,Sung-Chou Li

International journal of molecular sciences 23: PubMed36077082

2022

Combined Treatment with Curcumin and Ferulic Acid Suppressed the Aβ-Induced Neurotoxicity More than Curcumin and Ferulic Acid Alone.

Applications

Unspecified application

Species

Unspecified reactive species

Hideaki Ohashi,Mayumi Tsuji,Tatsunori Oguchi,Yutaro Momma,Tetsuhito Nohara,Naohito Ito,Ken Yamamoto,Miki Nagata,Atsushi Michael Kimura,Yuji Kiuchi,Kenjiro Ono

Bioengineered 12:2676-2687 PubMed34115550

2021

Overexpression of PLK1 relieved the myocardial ischemia-reperfusion injury of rats through inducing the mitophagy and regulating the p-AMPK/FUNDC1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Shan Mao,Shuning Tian,Xianghong Luo,Ming Zhou,Zheng Cao,Ji Li

Molecular medicine reports 22:4579-4588 PubMed33174051

2020

lncRNA FGD5 antisense RNA 1 upregulates RORA to suppress hypoxic injury of human cardiomyocyte cells by inhibiting oxidative stress and apoptosis via miR‑195.

Applications

Unspecified application

Species

Unspecified reactive species

Xinyong Cai,Ping Zhang,Shu Wang,Lang Hong,Songping Yu,Bin Li,Hong Zeng,Xu Yang,Liang Shao

EBioMedicine 50:274-289 PubMed31787569

2019

Human iPSC-derived astrocytes from ALS patients with mutated C9ORF72 show increased oxidative stress and neurotoxicity.

Applications

Unspecified application

Species

Unspecified reactive species

Anastasya Birger,Israel Ben-Dor,Miri Ottolenghi,Tikva Turetsky,Yaniv Gil,Sahar Sweetat,Liat Perez,Vitali Belzer,Natania Casden,Debora Steiner,Michal Izrael,Eithan Galun,Eva Feldman,Oded Behar,Benjamin Reubinoff

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human SOD2 ELISA Kit

Sandwich ELISA - Human SOD2 ELISA Kit (AB178012)

Sandwich ELISA - Human SOD2 ELISA Kit (AB178012)

Sandwich ELISA - Human SOD2 ELISA Kit (AB178012)

Sandwich ELISA - Human SOD2 ELISA Kit (AB178012)

Sandwich ELISA - Human SOD2 ELISA Kit (AB178012)

Western blot - Human SOD2 ELISA Kit (AB178012)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (6)

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