Human soluble EGFR ELISA Kit is a single-wash 90-min Simplestep used to quantify Human soluble EGFR with a sensitivity of 36.63 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
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- Biomedicines 10:2022Anticancer Effects with Molecular Docking Confirmation of Newly Synthesized Isatin Sulfonamide Molecular Hybrid Derivatives against Hepatic Cancer Cell Lines.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMahmoud Eldeeb,Eman F Sanad,Ahmed Ragab,Yousry A Ammar,Khaled Mahmoud,Mamdouh M Ali,Nadia M HamdyPubMed 35327524
Key facts
- Detection method
- Colorimetric
- Sample types
- Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 109.375 - 7000 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 36.63 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
The protein expressed by the EGFR gene functions as a receptor tyrosine kinase, binding with ligands from the EGF family to activate several signaling cascades that translate extracellular signals into cellular responses. Known ligands include EGF, TGFA/TGF-alpha, AREG, epigen, BTC/betacellulin, epiregulin, and HBEGF. Ligand binding triggers receptor dimerization and autophosphorylation, recruiting adapter proteins like GRB2 and activating complex downstream signaling pathways such as RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC, and STATs, and possibly the NF-kappa-B cascade. EGFR also directly phosphorylates proteins like RGS16 and MUC1, influencing various cellular processes including its coupling with G protein-coupled receptor signaling and cell migration through interaction with CCDC88A/GIV. Isoform 2 of EGFR may act as an antagonist to EGF actions. In microbial infection, EGFR also serves as a receptor facilitating hepatitis C virus (HCV) entry into hepatocytes by promoting CD81-CLDN1 receptor complexes and enhancing membrane fusion with HCV envelope glycoproteins. This supplementary information is collated from multiple sources and compiled automatically.
Alternative names
ERBB, ERBB1, HER1, EGFR, Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1
What's included?
Recommended products
Human soluble EGFR ELISA Kit is a single-wash 90-min Simplestep used to quantify Human soluble EGFR with a sensitivity of 36.63 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 109.375 - 7000 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 36.63 pg/mL
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Serum | n 3 | C.V. 2.4 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Serum | n 8 | C.V. 3.1 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 95 | Range 85 - 101 % |
Sample type Serum | Average % = 96 | Range 91 - 100 % |
Sample type EDTA Plasma | Average % = 96 | Range 94 - 97 % |
Sample type Heparin Plasma | Average % = 94 | Range 89 - 98 % |
Sample type Citrate plasma | Average % = 98 | Range 91 - 105 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human soluble EGFR ELISA Kit ab269558 is a rapid single-wash 90-min Sandwich ELISA to measure Human soluble EGFR in cell culture supernatant, citrate plasma, EDTA plasma, heparin plasma, serum. This SimpleStep sensitivity is 36.63 pg/mL.
How the assay works
Human soluble EGFR SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.
Assay Specificity
Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.
Human soluble EGFR ELISA Kit ab269558 protocol summary
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm
Soluble epidermal growth factor receptor (sEGFR) is the extracellular domain of the EGFR cell-surface receptor protein and is created either from alternative mRNA transcripts or from proteolytic cleavage of membrane-bound EGFR. sEGFR can compete with the full-length receptor, thus interfering with signaling cascades initiated through ligand binding. While its overall role is not fully understood, there is much consideration for using it as a diagnostic biomarker of cancerous tumors.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
EGFR or Epidermal Growth Factor Receptor is a transmembrane glycoprotein that acts as a receptor for members of the epidermal growth factor family. Known alternatively as ErbB1 or HER1 this receptor has an approximate molecular weight of 170 kDa. EGFR is expressed in various cell types notably on epithelial cells and can influence multiple cellular processes through its kinase activity. It participates in the regulation of cell growth multiplication and survival by activating its kinase domain upon ligand binding.
- Biological function summary
The EGFR protein plays an important role in cellular communication and signaling processes. EGFR pairs with other receptor family members to form active dimers or even higher-order complexes which in turn initiate intracellular signaling cascades. Through these complexes EGFR influences many processes including cell differentiation and repair. This function of EGFR makes it an integral part of mammalian biology affecting how cells respond to their environment by mediating changes in gene expression.
- Pathways
EGFR is a central player in the MAPK and PI3K/Akt signaling pathways. Alongside other protein partners like KRAS and PI3 kinase it contributes to transmitting signals from the cell surface to the nucleus affecting gene transcription and cell behavior. These pathways are important for normal cell growth and division and aberrations in these pathways can lead to excessive or insufficient cell proliferation.
- Associated diseases and disorders
EGFR is pertinent to cancer biology including non-small cell lung cancer and glioblastoma where mutations or overexpression of the receptor frequently occur. It connects to proteins such as PTEN and BRAF which influence tumor progression and response to targeted therapies. EGFR's involvement in these disorders highlights its significance as a therapeutic target since it can be manipulated to alter disease progression.
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6 product images
ELISA - Human soluble EGFR ELISA Kit (ab269558)
Sandwich ELISA - Human soluble EGFR ELISA Kit (ab269558)
Human soluble EGFR standard curve comparison.
Standard Curve comparison between human soluble EGFR SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
Sandwich ELISA - Human soluble EGFR ELISA Kit (ab269558)
Human soluble EGFR standard curve comparison.
Standard Curve comparison between the original human MMP3 SimpleStep ELISA kit (ab193764) and current human MMP3 SimpleStep ELISA kit (ab269558).
Sandwich ELISA - Human soluble EGFR ELISA Kit (ab269558)
Example of human soluble EGFR standard curve in Sample Diluent NS.
The soluble EGFR standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human soluble EGFR ELISA Kit (ab269558)
Interpolated concentrations of native soluble EGFR in human serum and plasma samples.
The concentrations of soluble EGFR were measured in duplicates, interpolated from the soluble EGFR standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 10%, plasma (citrate) 10%, plasma (EDTA) 10%, and plasma (heparin) 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean soluble EGFR concentration was determined to be 43 ng/mL in serum, 38 ng/mL in plasma (citrate), 34 ng/mL in plasma (EDTA), and 32 ng/mL in plasma (heparin).
Sandwich ELISA - Human soluble EGFR ELISA Kit (ab269558)
Interpolated concentrations of native soluble EGFR in human A431 cell culture supernatant samples.
The concentrations of soluble EGFR were measured in duplicates, interpolated from the soluble EGFR standard curves and corrected for sample dilution. Undiluted samples are as follows: A431 cell culture supernatant 2.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean soluble EGFR concentration was determined to be 146 ng/mL in A431 cell culture supernatant.
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