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AB269558

Human soluble EGFR ELISA Kit

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Human soluble EGFR ELISA Kit is a single-wash 90-min Simplestep used to quantify Human soluble EGFR with a sensitivity of 36.63 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

ERBB, ERBB1, HER1, EGFR, Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1

6 Images
ELISA - Human soluble EGFR ELISA Kit (AB269558)
  • ELISA

Supplier Data

ELISA - Human soluble EGFR ELISA Kit (AB269558)
Sandwich ELISA - Human soluble EGFR ELISA Kit (AB269558)
  • sELISA

Supplier Data

Sandwich ELISA - Human soluble EGFR ELISA Kit (AB269558)

Example of human soluble EGFR standard curve in Sample Diluent NS.

The soluble EGFR standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human soluble EGFR ELISA Kit (AB269558)
  • sELISA

Supplier Data

Sandwich ELISA - Human soluble EGFR ELISA Kit (AB269558)

Human soluble EGFR standard curve comparison.

Standard Curve comparison between human soluble EGFR SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

Sandwich ELISA - Human soluble EGFR ELISA Kit (AB269558)
  • sELISA

Supplier Data

Sandwich ELISA - Human soluble EGFR ELISA Kit (AB269558)

Interpolated concentrations of native soluble EGFR in human serum and plasma samples.

The concentrations of soluble EGFR were measured in duplicates, interpolated from the soluble EGFR standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 10%, plasma (citrate) 10%, plasma (EDTA) 10%, and plasma (heparin) 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean soluble EGFR concentration was determined to be 43 ng/mL in serum, 38 ng/mL in plasma (citrate), 34 ng/mL in plasma (EDTA), and 32 ng/mL in plasma (heparin).

Sandwich ELISA - Human soluble EGFR ELISA Kit (AB269558)
  • sELISA

Supplier Data

Sandwich ELISA - Human soluble EGFR ELISA Kit (AB269558)

Interpolated concentrations of native soluble EGFR in human A431 cell culture supernatant samples.

The concentrations of soluble EGFR were measured in duplicates, interpolated from the soluble EGFR standard curves and corrected for sample dilution. Undiluted samples are as follows : A431 cell culture supernatant 2.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean soluble EGFR concentration was determined to be 146 ng/mL in A431 cell culture supernatant.

Sandwich ELISA - Human soluble EGFR ELISA Kit (AB269558)
  • sELISA

Supplier Data

Sandwich ELISA - Human soluble EGFR ELISA Kit (AB269558)

Human soluble EGFR standard curve comparison.

Standard Curve comparison between the original human MMP3 SimpleStep ELISA kit (ab193764) and current human MMP3 SimpleStep ELISA kit (ab269558).

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 36.63 pg/mL

Range

109.375 - 7000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human soluble EGFR ELISA Kit ab269558 is a rapid single-wash 90-min Sandwich ELISA to measure Human soluble EGFR in cell culture supernatant, citrate plasma, EDTA plasma, heparin plasma, serum. This SimpleStep sensitivity is 36.63 pg/mL.

How the assay works

Human soluble EGFR SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human soluble EGFR ELISA Kit ab269558 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Soluble epidermal growth factor receptor (sEGFR) is the extracellular domain of the EGFR cell-surface receptor protein and is created either from alternative mRNA transcripts or from proteolytic cleavage of membrane-bound EGFR. sEGFR can compete with the full-length receptor, thus interfering with signaling cascades initiated through ligand binding. While its overall role is not fully understood, there is much consideration for using it as a diagnostic biomarker of cancerous tumors.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.1" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.4" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "85 - 101 %", "average": "= 95" }, { "sample": "Serum", "range": "91 - 100 %", "average": "= 96" }, { "sample": "EDTA Plasma", "range": "94 - 97 %", "average": "= 96" }, { "sample": "Heparin Plasma", "range": "89 - 98 %", "average": "= 94" }, { "sample": "Citrate plasma", "range": "91 - 105 %", "average": "= 98" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab269558-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB269558-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB269558-CMP06", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB269558-CMP10", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB269558-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB269558-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB269558-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human soluble EGFR Lyophilized Recombinant Protein", "number":"AB269558-CMP08", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human soluble EGFR Capture Antibody", "number":"AB269558-CMP02", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BI", "number":"AB269558-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human soluble EGFR Detector Antibody", "number":"AB269558-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EGFR or Epidermal Growth Factor Receptor is a transmembrane glycoprotein that acts as a receptor for members of the epidermal growth factor family. Known alternatively as ErbB1 or HER1 this receptor has an approximate molecular weight of 170 kDa. EGFR is expressed in various cell types notably on epithelial cells and can influence multiple cellular processes through its kinase activity. It participates in the regulation of cell growth multiplication and survival by activating its kinase domain upon ligand binding.
Biological function summary

The EGFR protein plays an important role in cellular communication and signaling processes. EGFR pairs with other receptor family members to form active dimers or even higher-order complexes which in turn initiate intracellular signaling cascades. Through these complexes EGFR influences many processes including cell differentiation and repair. This function of EGFR makes it an integral part of mammalian biology affecting how cells respond to their environment by mediating changes in gene expression.

Pathways

EGFR is a central player in the MAPK and PI3K/Akt signaling pathways. Alongside other protein partners like KRAS and PI3 kinase it contributes to transmitting signals from the cell surface to the nucleus affecting gene transcription and cell behavior. These pathways are important for normal cell growth and division and aberrations in these pathways can lead to excessive or insufficient cell proliferation.

EGFR is pertinent to cancer biology including non-small cell lung cancer and glioblastoma where mutations or overexpression of the receptor frequently occur. It connects to proteins such as PTEN and BRAF which influence tumor progression and response to targeted therapies. EGFR's involvement in these disorders highlights its significance as a therapeutic target since it can be manipulated to alter disease progression.
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Product protocols

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Target data

Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses (PubMed : 10805725, PubMed : 27153536, PubMed : 2790960, PubMed : 35538033). Known ligands include EGF, TGFA/TGF-alpha, AREG, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF (PubMed : 12297049, PubMed : 15611079, PubMed : 17909029, PubMed : 20837704, PubMed : 27153536, PubMed : 2790960, PubMed : 7679104, PubMed : 8144591, PubMed : 9419975). Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules (PubMed : 27153536). May also activate the NF-kappa-B signaling cascade (PubMed : 11116146). Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling (PubMed : 11602604). Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin (PubMed : 11483589). Positively regulates cell migration via interaction with CCDC88A/GIV which retains EGFR at the cell membrane following ligand stimulation, promoting EGFR signaling which triggers cell migration (PubMed : 20462955). Plays a role in enhancing learning and memory performance (By similarity). Plays a role in mammalian pain signaling (long-lasting hypersensitivity) (By similarity).. Isoform 2 may act as an antagonist of EGF action.. (Microbial infection) Acts as a receptor for hepatitis C virus (HCV) in hepatocytes and facilitates its cell entry. Mediates HCV entry by promoting the formation of the CD81-CLDN1 receptor complexes that are essential for HCV entry and by enhancing membrane fusion of cells expressing HCV envelope glycoproteins.
See full target information EGFR
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Biomedicines 10: PubMed35327524

2022

Anticancer Effects with Molecular Docking Confirmation of Newly Synthesized Isatin Sulfonamide Molecular Hybrid Derivatives against Hepatic Cancer Cell Lines.

Applications

Unspecified application

Species

Unspecified reactive species

Mahmoud Eldeeb,Eman F Sanad,Ahmed Ragab,Yousry A Ammar,Khaled Mahmoud,Mamdouh M Ali,Nadia M Hamdy
View all publications
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Product promise

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