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AB326389

Human soluble EGFR ELISA Kit, Chemiluminescent

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Human soluble EGFR ELISA Kit, Chemiluminescent is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Human soluble EGFR with higher sensitivity and broader measuring range.

- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 28.81 - 63000 pg/ml with a sensitivity of 7.82pg/mL.
- Design your own immunoassay: we also offer the conjugation-ready antibody pair.
-Patent pending

View Alternative Names

ERBB, ERBB1, HER1, EGFR, Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1

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Sandwich ELISA - Human soluble EGFR ELISA Kit, Chemiluminescent (AB326389)
  • sELISA

Lab

Sandwich ELISA - Human soluble EGFR ELISA Kit, Chemiluminescent (AB326389)

SimpleStep ELISA® Ignite technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 55 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

Sandwich ELISA - Human soluble EGFR ELISA Kit, Chemiluminescent (AB326389)
  • sELISA

Lab

Sandwich ELISA - Human soluble EGFR ELISA Kit, Chemiluminescent (AB326389)

Serum from ten individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean soluble EGFR concentration was determined to be 43 ng/mL with a range of 36 – 53 ng/mL.

Sandwich ELISA - Human soluble EGFR ELISA Kit, Chemiluminescent (AB326389)
  • sELISA

Lab

Sandwich ELISA - Human soluble EGFR ELISA Kit, Chemiluminescent (AB326389)

Dynamic range comparison between the Chemiluminescent Human soluble EGFR SimpleStep ELISA and colorimetric Human soluble EGFR SimpleStep ELISA (ab269558). The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and increased dynamic range.

Sandwich ELISA - Human soluble EGFR ELISA Kit, Chemiluminescent (AB326389)
  • sELISA

Lab

Sandwich ELISA - Human soluble EGFR ELISA Kit, Chemiluminescent (AB326389)

Standard curve comparison between the Chemiluminescent Human soluble EGFR SimpleStep ELISA and colorimetric Human soluble EGFR SimpleStep ELISA (ab269558) in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human soluble EGFR ELISA Kit, Chemiluminescent (AB326389)
  • sELISA

Supplier Data

Sandwich ELISA - Human soluble EGFR ELISA Kit, Chemiluminescent (AB326389)

Interpolated concentrations of native soluble EGFR in human A431 cell culture supernatant samples.

The concentrations of soluble EGFR were measured in duplicates interpolated from the soluble EGFR standard curves and corrected for sample dilution. Undiluted samples are as follows : A431 cell culture supernatant 2.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD n=2). The mean soluble EGFR concentration was determined to be 146 ng/mL in A431 cell culture supernatant.

Sandwich ELISA - Human soluble EGFR ELISA Kit, Chemiluminescent (AB326389)
  • sELISA

Supplier Data

Sandwich ELISA - Human soluble EGFR ELISA Kit, Chemiluminescent (AB326389)

Interpolated concentrations of native soluble EGFR in human serum and plasma samples.

The concentrations of soluble EGFR were measured in duplicates interpolated from the soluble EGFR standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 10% plasma (citrate) 10% plasma (EDTA) 10% and plasma (heparin) 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD n=2). The mean soluble EGFR concentration was determined to be 43 ng/mL in serum 38 ng/mL in plasma (citrate) 34 ng/mL in plasma (EDTA) and 32 ng/mL in plasma (heparin).

Key facts

Detection method

Luminescent

Sample types

Cell culture supernatant, Serum, EDTA Plasma, Heparin Plasma, Citrate plasma, Milk

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 7.82 pg/mL

Range

28.81 - 63000 pg/mL

Assay time

55m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

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Product details

Human soluble EGFR ELISA Kit, Chemiluminescent (ab326389) is a rapid single-wash sandwich 55-min ELISA to measure Human soluble EGFR in Cell culture supernatant, Serum, EDTA Plasma, Heparin Plasma, Citrate plasma with a sensitivity of 7.82 pg/mL.

How the assay works

Human soluble EGFR ELISA Kit, Chemiluminescent (ab326389) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human soluble EGFR (ab326389) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-sEGFR antibody [EPR22309-151] - BSA and Aside free (Capture) ab270342
- Anti-sEGFR antibody [EPR22309-159] - BSA and Aside free (Detector) ab270343
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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 2.4" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 3.1" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "85 - 101 %", "average": "= 95" }, { "sample": "Serum", "range": "91 - 100 %", "average": "= 96" }, { "sample": "EDTA Plasma", "range": "94 - 97 %", "average": "= 96" }, { "sample": "Heparin Plasma", "range": "89 - 98 %", "average": "= 94" }, { "sample": "Citrate plasma", "range": "91 - 105 %", "average": "= 98" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses (PubMed : 10805725, PubMed : 27153536, PubMed : 2790960, PubMed : 35538033). Known ligands include EGF, TGFA/TGF-alpha, AREG, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF (PubMed : 12297049, PubMed : 15611079, PubMed : 17909029, PubMed : 20837704, PubMed : 27153536, PubMed : 2790960, PubMed : 7679104, PubMed : 8144591, PubMed : 9419975). Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules (PubMed : 27153536). May also activate the NF-kappa-B signaling cascade (PubMed : 11116146). Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling (PubMed : 11602604). Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin (PubMed : 11483589). Positively regulates cell migration via interaction with CCDC88A/GIV which retains EGFR at the cell membrane following ligand stimulation, promoting EGFR signaling which triggers cell migration (PubMed : 20462955). Plays a role in enhancing learning and memory performance (By similarity). Plays a role in mammalian pain signaling (long-lasting hypersensitivity) (By similarity).. Isoform 2 may act as an antagonist of EGF action.. (Microbial infection) Acts as a receptor for hepatitis C virus (HCV) in hepatocytes and facilitates its cell entry. Mediates HCV entry by promoting the formation of the CD81-CLDN1 receptor complexes that are essential for HCV entry and by enhancing membrane fusion of cells expressing HCV envelope glycoproteins.
See full target information EGFR
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websiteProtocolBooklet
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Product promise

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