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AB100642

Human Soluble TNF Receptor I ELISA Kit

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(3 Publications)

Human Soluble TNF Receptor I ELISA Kit is a Sandwich ELISA for the measurement of Human Soluble TNF Receptor I in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

CD120a, TNFAR, TNFR1, TNFRSF1A, Tumor necrosis factor receptor superfamily member 1A, Tumor necrosis factor receptor 1, Tumor necrosis factor receptor type I, p55, p60, TNF-R1, TNF-RI, TNFR-I

1 Images
Sandwich ELISA - Human Soluble TNF Receptor I ELISA Kit (AB100642)
  • sELISA

Unknown

Sandwich ELISA - Human Soluble TNF Receptor I ELISA Kit (AB100642)

Representative standard curve using ab100642

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

< 1 pg/mL

Range

0.55 - 400 pg/mL

Assay Platform

Microplate

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's Human STNF-R1 (soluble tumor necrosis factor receptor I) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human STNF-R1 in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for Human STNF-R1 coated on a 96-well plate. Standards and samples are pipetted into the wells and STNF-R1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human STNF-R1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of STNF-R1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Optimization may be required with urine samples.

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Recovery

[ { "sample": "Cell culture supernatant", "range": "71 - 90 %", "average": "= 80.02" }, { "sample": "Serum", "range": "72 - 91 %", "average": "= 80.43" }, { "sample": "Plasma", "range": "73 - 91 %", "average": "= 81.36" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100642-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100642-CMP01", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100642-CMP07", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin Concentrate", "number":"AB100642-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Soluble TNF Receptor I Microplate (12 x 8 wells)", "number":"AB100642-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Human Soluble TNF Receptor I", "number":"AB100642-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Human Soluble TNF Receptor I Standard (lyophilized)", "number":"AB100642-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100642-CMP09", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent C", "number":"AB100642-CMP04", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB100642-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

Receptor for TNFSF2/TNF and homotrimeric TNFSF1/lymphotoxin-alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Contributes to the induction of non-cytocidal TNF effects including anti-viral state and activation of the acid sphingomyelinase.
See full target information TNFRSF1A
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 12:592727 PubMed33968010

2021

Increased Serum Levels of Soluble TNF-α Receptor Is Associated With ICU Mortality in COVID-19 Patients.

Applications

Unspecified application

Species

Unspecified reactive species

Esmaeil Mortaz,Payam Tabarsi,Hamidreza Jamaati,Neda Dalil Roofchayee,Neda K Dezfuli,Seyed MohammadReza Hashemian,Afshin Moniri,Majid Marjani,Majid Malekmohammad,Davood Mansouri,Mohammad Varahram,Gert Folkerts,Ian M Adcock

Cellular and molecular bioengineering 13:487-505 PubMed33184579

2020

Cholinergic Activation of Primary Human Derived Intestinal Epithelium Does Not Ameliorate TNF-α Induced Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Sanjin Hosic,Will Lake,Eric Stas,Ryan Koppes,David T Breault,Shashi K Murthy,Abigail N Koppes

Nephrology, dialysis, transplantation : official p 29:926-33 PubMed24452849

2014

Short-term efficacy and safety of sitagliptin treatment in long-term stable renal recipients with new-onset diabetes after transplantation.

Applications

Unspecified application

Species

Unspecified reactive species

Thea Anine Strøm Halden,Anders Åsberg,Karen Vik,Anders Hartmann,Trond Jenssen
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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