Human Soluble TNF Receptor I ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human Soluble TNF Receptor I in Human in Plasma, Cell culture supernatant, Serum samples.
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- Frontiers in immunology 12:5927272021Increased Serum Levels of Soluble TNF-α Receptor Is Associated With ICU Mortality in COVID-19 Patients.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEsmaeil Mortaz,Payam Tabarsi,Hamidreza Jamaati,Neda Dalil Roofchayee,Neda K Dezfuli,Seyed MohammadReza Hashemian,Afshin Moniri,Majid Marjani,Majid Malekmohammad,Davood Mansouri,Mohammad Varahram,Gert Folkerts,Ian M AdcockPubMed 33968010
- Cellular and molecular bioengineering 13:487-5052020Cholinergic Activation of Primary Human Derived Intestinal Epithelium Does Not Ameliorate TNF-α Induced Injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSanjin Hosic et. al.PubMed 33184579
- Nephrology, dialysis, transplantation : official p 29:926-332014Short-term efficacy and safety of sitagliptin treatment in long-term stable renal recipients with new-onset diabetes after transplantation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesThea Anine Strøm Halden et. al.PubMed 24452849
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 0.55 - 400 pg/mL
- Sensitivity
- < 1 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Receptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Contributes to the induction of non-cytocidal TNF effects including anti-viral state and activation of the acid sphingomyelinase.
Alternative names
CD120a, TNFAR, TNFR1, TNFRSF1A, Tumor necrosis factor receptor superfamily member 1A, Tumor necrosis factor receptor 1, Tumor necrosis factor receptor type I, p55, p60, TNF-R1, TNF-RI, TNFR-I
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Human Soluble TNF Receptor I ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human Soluble TNF Receptor I in Human in Plasma, Cell culture supernatant, Serum samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 0.55 - 400 pg/mL
- Assay Platform
- Microplate
- Sensitivity
- < 1 pg/mL
- General recovery
- 80%
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 80.02 | Range 71 - 90 % |
Sample type Serum | Average % = 80.43 | Range 72 - 91 % |
Sample type Plasma | Average % = 81.36 | Range 73 - 91 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Abcam's Human STNF-R1 (soluble tumor necrosis factor receptor I) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human STNF-R1 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human STNF-R1 coated on a 96-well plate. Standards and samples are pipetted into the wells and STNF-R1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human STNF-R1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of STNF-R1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimization may be required with urine samples.
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1 product image
Sandwich ELISA - Human Soluble TNF Receptor I ELISA Kit (ab100642)
Representative standard curve using ab100642
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Product protocols
- Visit the general protocols
- Visit troubleshooting
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