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AB100643

Human Soluble TNF Receptor II ELISA Kit

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(1 Publication)

Human Soluble TNF Receptor II ELISA Kit is a sandwich ELISA designed to quantify Human Soluble TNF Receptor II with a sensitivity of 5 pg/mL.

- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 2.74 - 2000 pg/mL
2 Images
Sandwich ELISA - Human Soluble TNF Receptor II ELISA Kit (AB100643)
  • sELISA

Unknown

Sandwich ELISA - Human Soluble TNF Receptor II ELISA Kit (AB100643)

Representative standard curve using ab100643

Sandwich ELISA - Human Soluble TNF Receptor II ELISA Kit (AB100643)
  • sELISA

Unknown

Sandwich ELISA - Human Soluble TNF Receptor II ELISA Kit (AB100643)

Representative standard curve using ab100643

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

< 5 pg/mL

Range

2.74 - 2000 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Soluble TNF Receptor II ELISA Kit ab100643 is a sandwich ELISA to measure Human Soluble TNF Receptor II in serum, plasma, cell culture supernatant with a sensitivity of 5 pg/ml.

How the assay works

This assay employs an antibody specific for Human Soluble TNF Receptor II coated on a 96-well plate. Standards and samples are pipetted into the wells and Soluble TNF Receptor II present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Soluble TNF Receptor II antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Soluble TNF Receptor II bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Assay Specificity

Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details

Human Soluble TNF Receptor II ELISA Kit ab100643 protocol summary

1. Add standard or sample to each well used. Incubate at room temperature
2. Wash and add prepared biotinylated antibody to each well. Incubate at room temperature.
3. Wash and add prepared streptavidin solution. Incubate at room temperature.
4. Add TMB Development Solution to each well. Incubate at room temperature.
5. Add Stop Solution to each well. Read at 450nm immediately.

Optimization may be required with urine samples.

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Recovery

[ { "sample": "Cell culture supernatant", "range": "91 - 112 %", "average": "= 102.65" }, { "sample": "Serum", "range": "95 - 115 %", "average": "= 104.92" }, { "sample": "Plasma", "range": "94 - 118 %", "average": "= 105.49" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100643-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100643-CMP01", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100643-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Soluble TNF Receptor II Microplate (12 x 8 wells)", "number":"AB100643-CMP05", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB100643-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Human Soluble TNF Receptor II Standard (lyophilized)", "number":"AB100643-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100643-CMP09", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB100643-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Human Soluble TNF Receptor II", "number":"AB100643-CMP08", "productcode":"" }, { "size":"1 x 200 µL", "name":"500X HRP-Streptavidin Concentrate", "number":"AB100643-CMP02", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Soluble TNF Receptor II also known as TNFRSF1B or CD120b acts as a decoy receptor that binds with tumor necrosis factor-alpha (TNF-α) in the serum and extracellular space. It has a molecular mass of approximately 75 kDa. This receptor is expressed in various tissues including the immune cells and plays a role in regulating inflammatory responses. By binding TNF-α Soluble TNF Receptor II prevents TNF-α from interacting with cell surface receptors modulating its inflammatory effects.
Biological function summary

Soluble TNF Receptor II modulates inflammation and immune response. It is a part of the TNF receptor superfamily and can take part in forming a receptor-ligand complex with TNF-α impacting the downstream signaling. This receptor acts to neutralize TNF-α activity by preventing its engagement with pro-inflammatory receptors on cell surfaces. Apart from its inhibitory role it also assists in the clearance of TNF-α from the circulation managing excess TNF-α levels in the body.

Pathways

Soluble TNF Receptor II is dominant in the TNF signaling pathway and the NF-κB pathway. These pathways manage immune response cell survival and apoptosis. TNFR1 another receptor in the TNF family plays a related role in the TNF signaling pathway and often collaborates with Soluble TNF Receptor II to regulate balance between cell death and cell survival. The complex formed by Soluble TNF Receptor II and TNF-α diminishes excessive inflammation by reducing active TNF-α available for binding with TNFR1.

Soluble TNF Receptor II holds significance in rheumatoid arthritis and inflammatory bowel disease. Its presence modifies the course of these diseases by modulating the levels and effects of TNF-α. The inhibition of TNF-α-driven inflammation through interaction with Soluble TNF Receptor II can reduce tissue damage and improve symptoms. In these contexts it shows links with TNF-α which is a well-known mediator of inflammation and plays a role in the pathophysiology of these inflammatory disorders.
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Product protocols

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Target data

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Arthritis research & therapy 18:106 PubMed27180164

2016

Identification of definitive serum biomarkers associated with disease activity in primary Sjögren's syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Ayumi Nishikawa,Katsuya Suzuki,Yoshiaki Kassai,Yuumi Gotou,Maiko Takiguchi,Takahiro Miyazaki,Keiko Yoshimoto,Hidekata Yasuoka,Kunihiro Yamaoka,Rimpei Morita,Akihiko Yoshimura,Tsutomu Takeuchi
View all publications
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websiteProtocolBooklet
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