Human Sonic Hedgehog ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human Sonic Hedgehog in Human in Plasma, Cell culture supernatant, Serum samples.
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- Neuron 111:13482023VEGF Mediates Commissural Axon Chemoattraction through Its Receptor Flk1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCarmen Ruiz de Almodovar,Pierre J Fabre,Ellen Knevels,Cathy Coulon,Inmaculada Segura,Patrick C G Haddick,Liesbeth Aerts,Nicolas Delattin,Geraldine Strasser,Won-Jong Oh,Christian Lange,Stefan Vinckier,Jody Haigh,Coralie Fouquet,Chengua Gu,Kari Alitalo,Valerie Castellani,Marc Tessier-Lavigne,Alain Chedotal,Frederic Charron,Peter CarmelietPubMed 37080170
- Respiratory research 23:862022C/EBP homologous protein promotes Sonic Hedgehog secretion from type II alveolar epithelial cells and activates Hedgehog signaling pathway of fibroblast in pulmonary fibrosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaoyu Yang,Wei Sun,Xiaoyan Jing,Qian Zhang,Hui Huang,Zuojun XuPubMed 35395850
- Scientific reports 12:53412022In vitro ballooned hepatocytes can be produced by primary human hepatocytes and hepatic stellate cell sheets.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNobuhiro Hasui,Katsuhisa Sakaguchi,Tetsuya Ogawa,Yoshihiro Sakamoto,Tatsuya ShimizuPubMed 35351975
- Drug delivery 29:889-9052022Bone-targeted nanoplatform enables efficient modulation of bone tumor microenvironment for prostate cancer bone metastasis treatment.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiangyu Zhang,Qingbin Liu,Tingting Zhang,Pei Gao,Hui Wang,Lu Yao,Jingwen Huang,Shulong JiangPubMed 35285760
- Frontiers in oncology 11:7476922021High Circulating Sonic Hedgehog Protein Is Associated With Poor Outcome in EGFR-Mutated Advanced NSCLC Treated With Tyrosine Kinase Inhibitors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPaul Takam Kamga,Aurélie Swalduz,Adrien Costantini,Catherine Julié,Jean-François Emile,Maurice Pérol,Virginie Avrillon,Sandra Ortiz-Cuaran,Pierre de Saintigny,Etienne Giroux LeprieurPubMed 34970481
- Journal of pancreatic cancer 7:39-472021Cholesterol and CDON Regulate Sonic Hedgehog Release from Pancreatic Cancer Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJennifer I Alexander,Esteban Martinez,Alberto Vargas,Daniel Zinshteyn,Valerie Sodi,Denise C Connolly,Tiffiney R Hartman,Alana M O'ReillyPubMed 34235374
- Cancers 13:2021Baseline Hedgehog Pathway Activation and Increase of Plasma Wnt1 Protein Are Associated with Resistance to Immune Checkpoint Inhibitors in Advanced Non-Small-Cell Lung Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCamille Mehlman,Paul Takam Kamga,Adrien Costantini,Catherine Julié,Coraline Dumenil,Jennifer Dumoulin,Julia Ouaknine,Violaine Giraud,Thierry Chinet,Jean-François Emile,Etienne Giroux LeprieurPubMed 33807552
- JCI insight 4:2019Sonic hedgehog connects podocyte injury to mesangial activation and glomerulosclerosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDong Zhou et. al.PubMed 31647783
- FASEB journal : official publication of the Federa 33:12576-125872019Early activation of fibroblasts is required for kidney repair and regeneration after injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDong Zhou et. al.PubMed 31461626
- Hepatology communications 3:883-8932019Nocturnal Hypoxia Activation of the Hedgehog Signaling Pathway Affects Pediatric Nonalcoholic Fatty Liver Disease Severity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShikha S Sundaram et. al.PubMed 31334441
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 13.72 - 10000 pg/mL
- Sensitivity
- < 20 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Sonic hedgehog protein. The C-terminal part of the sonic hedgehog protein precursor displays an autoproteolysis and a cholesterol transferase activity (By similarity). Both activities result in the cleavage of the full-length protein into two parts (ShhN and ShhC) followed by the covalent attachment of a cholesterol moiety to the C-terminal of the newly generated ShhN (By similarity). Both activities occur in the reticulum endoplasmic (By similarity). Once cleaved, ShhC is degraded in the endoplasmic reticulum (By similarity). Sonic hedgehog protein N-product. The dually lipidated sonic hedgehog protein N-product (ShhNp) is a morphogen which is essential for a variety of patterning events during development. Induces ventral cell fate in the neural tube and somites (PubMed:24863049). Involved in the patterning of the anterior-posterior axis of the developing limb bud (By similarity). Essential for axon guidance (By similarity). Binds to the patched (PTCH1) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes (PubMed:10753901). In the absence of SHH, PTCH1 represses the constitutive signaling activity of SMO (PubMed:10753901).
Alternative names
Sonic hedgehog protein, SHH, HHG-1, Shh unprocessed N-terminal signaling and C-terminal autoprocessing domains, ShhNC
What's included?
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Human Sonic Hedgehog ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human Sonic Hedgehog in Human in Plasma, Cell culture supernatant, Serum samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 13.72 - 10000 pg/mL
- Assay Platform
- Microplate
- Sensitivity
- < 20 pg/mL
- General recovery
- 94%
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 91.72 | Range 82 - 101 % |
Sample type Serum | Average % = 95.34 | Range 84 - 103 % |
Sample type Plasma | Average % = 76.43 | Range 68 - 89 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Abcam's Sonic Hedgehog Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Sonic Hedgehog in serum, plasma, cell culture supernatants.
This assay employs an antibody specific for Human Sonic Hedgehog coated on a 96-well plate. Standards and samples are pipetted into the wells and Sonic Hedgehog present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Sonic Hedgehog antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Sonic Hedgehog bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimization may be required with urine samples.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Sonic Hedgehog also known as SHH is a protein that plays an important role in cell signaling during embryonic development. It has alternative names like 'HHG-1' and '5h4'. The Sonic Hedgehog protein is about 45 kDa in mass. Scientists find SHH expression in various tissues including the brain lungs and limb buds. Its activity plays a role in the organization of several tissue structures during development helping to regulate cellular growth and differentiation processes.
- Biological function summary
Sonic Hedgehog is critical for the vertebrate embryonic patterning and organogenesis. It functions as a signaling molecule and is part of the Hedgehog signaling complex. The protein acts by binding to the Patched receptor further influencing the Smoothened transducer in cellular signaling cascades. This signaling contributes to the proper formation of limbs central nervous system structures and other vital organs in early development stages.
- Pathways
Sonic Hedgehog participates in essential signaling pathways such as the Hedgehog pathway and the Wnt signaling pathway. It interacts with proteins including the Patched and Smoothened to facilitate downstream genetic transcription regulation. Through these pathways Sonic Hedgehog influences the transcription of specific genes that control cell growth. These interactions contribute to the regulation of different cell types and the organization of distinct tissue areas.
- Associated diseases and disorders
Sonic Hedgehog connects to issues like cancer and congenital malformations. Aberrations in its signaling can lead to conditions such as basal cell carcinoma and holoprosencephaly. Sonic Hedgehog through its influence on the Hedgehog signaling pathway implicates interactions with other proteins like GLI transcription factors and Patched. These interactions can dysregulate cellular processes and result in disease states when not functioning correctly.
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Sandwich ELISA - Human Sonic Hedgehog ELISA Kit (ab100639)
Representative standard curve using ab100639
Sandwich ELISA - Human Sonic Hedgehog ELISA Kit (ab100639)
Representative standard curve using ab100639
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