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AB233613

Human Sorbitol Dehydrogenase ELISA Kit

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Human Sorbitol Dehydrogenase ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Sorbitol Dehydrogenase with a sensitivity of 60 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Sorbitol dehydrogenase, SDH, L-iditol 2-dehydrogenase, Polyol dehydrogenase, Ribitol dehydrogenase, Xylitol dehydrogenase, RDH, XDH, SORD

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Sandwich ELISA - Human Sorbitol Dehydrogenase ELISA Kit (AB233613)
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Supplier Data

Sandwich ELISA - Human Sorbitol Dehydrogenase ELISA Kit (AB233613)

Interpolated concentrations of native Sorbitol Dehydrogenase in human serum, and plasma samples.

The concentrations of Sorbitol Dehydrogenase were measured in duplicates, interpolated from the Sorbitol Dehydrogenase standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 12.5%, and plasma (citrate) 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Sorbitol Dehydrogenase concentration was determined to be 19 ng/mL in serum and 16 ng/mL in plasma(citrate).

Sandwich ELISA - Human Sorbitol Dehydrogenase ELISA Kit (AB233613)
  • sELISA

Supplier Data

Sandwich ELISA - Human Sorbitol Dehydrogenase ELISA Kit (AB233613)

Serum from ten individual healthy human female donors was measured in duplicate.

Interpolated concentrations of native Sorbitol Dehydrogenase in human Jurkat cell extract and human liver tissue extract samples.

Sandwich ELISA - Human Sorbitol Dehydrogenase ELISA Kit (AB233613)
  • sELISA

Supplier Data

Sandwich ELISA - Human Sorbitol Dehydrogenase ELISA Kit (AB233613)

Example of human Sorbitol Dehydrogenase standard curve in Sample Diluent NS.

The Sorbitol Dehydrogenase standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Sorbitol Dehydrogenase ELISA Kit (AB233613)
  • sELISA

Supplier Data

Sandwich ELISA - Human Sorbitol Dehydrogenase ELISA Kit (AB233613)

Interpolated concentrations of spiked Sorbitol Dehydrogenase in human serum, plasma and urine samples.

The concentrations of Sorbitol Dehydrogenase were measured in duplicates, interpolated from the Sorbitol Dehydrogenase standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 10%, plasma (citrate) 25%, and urine 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Sandwich ELISA - Human Sorbitol Dehydrogenase ELISA Kit (AB233613)
  • sELISA

Supplier Data

Sandwich ELISA - Human Sorbitol Dehydrogenase ELISA Kit (AB233613)

Interpolated concentrations of native Sorbitol Dehydrogenase in human Jurkat cell extract and human liver tissue extract samples.

The concentrations of Sorbitol Dehydrogenase were measured in duplicate and interpolated from the Sorbitol Dehydrogenase standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Sorbitol Dehydrogenase concentration was determined to be 0.06 ng/μg in Jurkat cell extract and 3.3 ng/μg in liver tissue extract.

Sandwich ELISA - Human Sorbitol Dehydrogenase ELISA Kit (AB233613)
  • sELISA

Unknown

Sandwich ELISA - Human Sorbitol Dehydrogenase ELISA Kit (AB233613)

Example of human Sorbitol Dehydrogenase standard curve in 1X Cell Extraction Buffer.

The Sorbitol Dehydrogenase standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Urine, Cell culture extracts, Tissue Extracts, Citrate plasma, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 60 pg/mL

Range

0.39 - 25 ng/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Sorbitol Dehydrogenase ELISA Kit (ab233613) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Sorbitol Dehydrogenase protein in cit plasma, serum, tissue extracts, urine, and cell culture extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Sorbitol Dehydrogenase with 60 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Jurkat", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.9" }, { "reproducibilityType": "Intra", "sample": "Jurkat", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.3" } ]
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Recovery

[ { "sample": "Serum", "range": "92 - 97 %", "average": "= 95" }, { "sample": "Urine", "range": "102 - 113 %", "average": "= 107" }, { "sample": "Cell culture extracts", "range": "89 - 109 %", "average": "= 99" }, { "sample": "Citrate plasma", "range": "81 - 90 %", "average": "= 86" }, { "sample": "Tissue Culture Media", "range": "92 - 114 %", "average": "= 103" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Sorbitol Dehydrogenase (SDH) also known as SORD is an enzyme that catalyzes the oxidation of sorbitol to fructose. This reaction involves the conversion of a secondary alcohol group to a ketone. SDH has a molecular mass of approximately 38 kDa. It is expressed in various human tissues most importantly in the liver kidneys and seminal vesicles. The enzyme requires NAD+ as a cofactor for its catalytic activity.
Biological function summary

SDH plays a part in the polyol pathway which is important for the metabolism of glucose into fructose. It functions alongside aldose reductase to regulate the conversion of glucose into sorbitol and then sorbitol into fructose. SDH acts independently without forming a complex with other proteins. The amount and activity of SDH can affect cellular osmotic balance and energy storage.

Pathways

SDH is involved in the polyol pathway and influences sorbitol levels. An excess of sorbitol can contribute to cellular damage especially in the context of diabetes. SDH’s role is linked to the exclusive conversion of sorbitol to fructose contrasting with aldose reductase which initiates sorbitol production from glucose. Both enzymes are key in maintaining the balance between extracellular and intracellular glucose.

Alterations in SDH activity relate to diabetic complications like diabetic retinopathy and neuropathy. The accumulation of sorbitol due to insufficient SDH activity contributes to the development of these conditions. SDH's connection with aldose reductase is particularly notable here as both proteins impact the sorbitol levels that affect various tissues under hyperglycemic conditions.
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Product protocols

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Target data

Polyol dehydrogenase that catalyzes the reversible NAD(+)-dependent oxidation of various sugar alcohols. Is mostly active with D-sorbitol (D-glucitol), L-threitol, xylitol and ribitol as substrates, leading to the C2-oxidized products D-fructose, L-erythrulose, D-xylulose, and D-ribulose, respectively (PubMed : 3365415). Is a key enzyme in the polyol pathway that interconverts glucose and fructose via sorbitol, which constitutes an important alternate route for glucose metabolism. The polyol pathway is believed to be involved in the etiology of diabetic complications, such as diabetic neuropathy and retinopathy, induced by hyperglycemia (PubMed : 12962626, PubMed : 25105142, PubMed : 29966615). May play a role in sperm motility by using sorbitol as an alternative energy source for sperm motility (PubMed : 16278369). May have a more general function in the metabolism of secondary alcohols since it also catalyzes the stereospecific oxidation of (2R,3R)-2,3-butanediol. To a lesser extent, can also oxidize L-arabinitol, galactitol and D-mannitol and glycerol in vitro. Oxidizes neither ethanol nor other primary alcohols. Cannot use NADP(+) as the electron acceptor (PubMed : 3365415).
See full target information SORD
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websiteProtocolBooklet
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