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AB155440

Human SOST ELISA Kit

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(1 Publication)

Human SOST ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human SOST in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

UNQ2976/PRO7455/PRO7476, SOST, Sclerostin

1 Images
Sandwich ELISA - Human SOST ELISA Kit (AB155440)
  • sELISA

Unknown

Sandwich ELISA - Human SOST ELISA Kit (AB155440)

SOST Human ELISA Kit.

Representative standard curve using ab155440

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

< 15 pg/mL

Range

0.017 - 10 ng/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's SOST Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human SOST in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for Human SOST coated on a 96-well plate. Standards and samples are pipetted into the wells and SOST present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human SOST antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of SOST bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Optimization may be required with urine samples.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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Recovery

[ { "sample": "Serum", "range": "110 - 136 %", "average": "= 123.3" }, { "sample": "Plasma", "range": "118 - 134 %", "average": "= 125.7" }, { "sample": "Cell culture media", "range": "86 - 105 %", "average": "= 96.04" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab155440-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB155440-CMP01", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB155440-CMP06", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB155440-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Human SOST Standard (lyophilized)", "number":"AB155440-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SOST Microplate (12 x 8 wells)", "number":"AB155440-CMP04", "productcode":"" }, { "size":"1 x 200 µL", "name":"500X HRP-Streptavidin Concentrate", "number":"AB155440-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Human SOST", "number":"AB155440-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB155440-CMP08", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

Negative regulator of bone growth that acts through inhibition of Wnt signaling and bone formation.
See full target information SOST
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of thoracic disease 14:1563-1577 PubMed35693595

2022

Sclerostin aggravates cardiac remodeling after myocardial infarction by inhibition of Wnt/β-catenin signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Shuxin Zheng,Jinyi Wei,Peipei Chen,Fangliang Chen,Guang Yang
View all publications
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websiteProtocolBooklet
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