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AB314352

Human SOX17 ELISA Kit

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Human SOX17 ELISA Kit is a Sandwich ELISA for the measurement of Human SOX17 in Human in Cell Culture Media, Biofluids samples.

View Alternative Names

Transcription factor SOX-17, SOX17

1 Images
Sandwich ELISA - Human SOX17 ELISA Kit (AB314352)
  • sELISA

Supplier Data

Sandwich ELISA - Human SOX17 ELISA Kit (AB314352)

Example of standard curve using ab314352. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Plasma, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.03 ng/mL

Range

0.03 - 25 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

Product details

Human SOX17 ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human SOX17 in serum (human SOX17 concentration is low in normal serum/plasma, and may not be detectable in this assay concentration), plasma (collect plasma using EDTA and Citrate as an anticoagulant. Heparin is not recommended as anticoagulants for use in this assay), and cell culture supernatants. This assay employs an antibody specific for human SOX17 coated on a 96-well plate.

Recovery

[ { "sample": "Serum", "range": "67 - 79 %", "average": "= 72.13" }, { "sample": "Plasma", "range": "71 - 75 %", "average": "= 72.73" }, { "sample": "Cell culture media", "range": "76 - 144 %", "average": "= 113.1" } ]

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab314352-1x96Tests", "assetComponentDetails": [ { "size":"1 x 15 mL", "name":"5X Assay Diluent B (Item E)", "number":"AB314352-CMP06", "productcode":"" }, { "size":"1 x 200 µL", "name":"300X HRP-Streptavidin", "number":"AB314352-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human SOX17 Antibody-coated ELISA Plate", "number":"AB314352-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human SOX17 Protein Standard", "number":"AB314352-CMP11", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human SOX17 Detection Antibody", "number":"AB314352-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB314352-CMP08", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB314352-CMP07", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB314352-CMP04", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A (Item D)", "number":"AB314352-CMP05", "productcode":"" } ] } } }

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Product protocols

Target data

Acts as a transcription regulator that binds target promoter DNA (PubMed : 33952808). Binds to the sequences 5'-AACAAT-'3 or 5'-AACAAAG-3'. Modulates transcriptional regulation via WNT3A. Inhibits Wnt signaling. Promotes degradation of activated CTNNB1. Plays a key role in the regulation of embryonic development. Required for normal development of the definitive gut endoderm. Required for normal looping of the embryonic heart tube. Plays an important role in embryonic and postnatal vascular development, including development of arteries. Plays an important role in postnatal angiogenesis, where it is functionally redundant with SOX18. Required for the generation and maintenance of fetal hematopoietic stem cells, and for fetal hematopoiesis. Probable transcriptional activator in the premeiotic germ cells.
See full target information SOX17
websiteProtocolBooklet
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