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Human SOX17 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human SOX17 in Human in Cell culture supernatant, Plasma, Serum samples.

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Images

Sandwich ELISA - Human SOX17 ELISA Kit (AB314352), expandable thumbnail

Key facts

Detection method
Colorimetric
Sample types
Cell culture supernatant, Plasma, Serum
Assay type
Sandwich (quantitative)
Reactive species
Human
Range
0.03 - 25 ng/mL
Sensitivity
= 0.03 ng/mL

Reactivity data

Application
sELISA
Reactivity
Reacts
Dilution info
-
Notes

-

Target data

Function

Acts as a transcription regulator that binds target promoter DNA and bends the DNA. Binds to the sequences 5'-AACAAT-'3 or 5'-AACAAAG-3'. Modulates transcriptional regulation via WNT3A. Inhibits Wnt signaling. Promotes degradation of activated CTNNB1. Plays a key role in the regulation of embryonic development. Required for normal development of the definitive gut endoderm. Required for normal looping of the embryonic heart tube. Plays an important role in embryonic and postnatal vascular development, including development of arteries. Plays an important role in postnatal angiogenesis, where it is functionally redundant with SOX18. Required for the generation and maintenance of fetal hematopoietic stem cells, and for fetal hematopoiesis. Probable transcriptional activator in the premeiotic germ cells.

Alternative names

What's included?

1 x 96 Tests
Components
20X Wash Buffer
1 x 25 mL
300X HRP-Streptavidin
1 x 200 µL
5X Assay Diluent B (Item E)
1 x 15 mL
Assay Diluent A (Item D)
1 x 30 mL
Biotinylated Human SOX17 Detection Antibody
2 x 1 Vial
Human SOX17 Antibody-coated ELISA Plate
1 x 1 Unit
Lyophilized Human SOX17 Protein Standard
2 x 1 Vial
Stop Solution; 0.2M Sulfuric Acid
1 x 8 mL
TMB One-Step Substrate Reagent
1 x 12 mL

Recommended products

Human SOX17 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human SOX17 in Human in Cell culture supernatant, Plasma, Serum samples.

Key facts

Detection method
Colorimetric
Sample types
Cell culture supernatant, Plasma, Serum
Assay type
Sandwich (quantitative)
Reactive species
Human
Range
0.03 - 25 ng/mL
Assay Platform
Pre-coated microplate (12 x 8 well strips)
Sensitivity
= 0.03 ng/mL

Recovery

Sample specific recovery

Sample type
Serum
Average %
= 72.13
Range
67 - 79 %
Sample type
Plasma
Average %
= 72.73
Range
71 - 75 %
Sample type
Cell culture media
Average %
= 113.1
Range
76 - 144 %

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Notes

Human SOX17 ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human SOX17 in serum (human SOX17 concentration is low in normal serum/plasma, and may not be detectable in this assay concentration), plasma (collect plasma using EDTA and Citrate as an anticoagulant. Heparin is not recommended as anticoagulants for use in this assay), and cell culture supernatants. This assay employs an antibody specific for human SOX17 coated on a 96-well plate.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Sandwich ELISA - Human SOX17 ELISA Kit (ab314352), expandable thumbnail

    Sandwich ELISA - Human SOX17 ELISA Kit (ab314352)

    Example of standard curve using ab314352. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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