AB245707

Human SOX2 ELISA Kit

Recombinant
SimpleStep
What is this?

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(1 Publication)

Human SOX2 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human SOX2 with a sensitivity of 31 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Transcription factor SOX-2, SOX2

3 Images

ELISA

Supplier Data

ELISA - Human SOX2 ELISA Kit (AB245707)

sELISA

Supplier Data

Sandwich ELISA - Human SOX2 ELISA Kit (AB245707)

Example of human SOX2 standard curve in 1X Cell Extraction Buffer PTR. The SOX2 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human SOX2 ELISA Kit (AB245707)

Interpolated concentrations of native SOX2 in human NCCIT cell extracts based on a 50 μg/mL extract load. The concentrations of SOX2 were measured in duplicate and interpolated from the SOX2 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean SOX2 concentration was determined to be 36.48 ng/mL.

Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 31 pg/mL

Range

0.46875 - 30 ng/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human SOX2 ELISA Kit ab245707 is a rapid single-wash 90-min Sandwich ELISA to measure Human SOX2 in cell culture extracts. This SimpleStep sensitivity is 31 pg/mL.

How the assay works

Human SOX2 SimpleStep ELISA^{^®}employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^{^®} plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA^{^®} protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA^® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human SOX2 ELISA Kit ab245707 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-SOX2 antibody [EPR20531-102] - BSA and Azide free (Capture) ab252657
- Anti-SOX2 antibody [EPR20531-143] - BSA and Azide free (Detector) ab252657

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Cell Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.1" }, { "reproducibilityType": "Intra", "sample": "Cell Extract", "replicates": 24, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.7" } ]

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Recovery

[ { "sample": "Cell culture extracts", "range": "79 - 98 %", "average": "= 88" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

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Target data

The protein expressed by the gene SOX2 is critical for early embryogenesis and for the pluripotency of embryonic stem cells (PubMed : 18035408). This supplementary information is collated from multiple sources and compiled automatically.

See full target information SOX2

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 14:3366 PubMed37291242

2023

Bioinspired engineering of fusogen and targeting moiety equipped nanovesicles.

Applications

Unspecified application

Species

Unspecified reactive species

Lixue Wang,Guosheng Wang,Wenjun Mao,Yundi Chen,Md Mofizur Rahman,Chuandong Zhu,Peter M Prisinzano,Bo Kong,Jing Wang,Luke P Lee,Yuan Wan

View all publications

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human SOX2 ELISA Kit

ELISA - Human SOX2 ELISA Kit (AB245707)

Sandwich ELISA - Human SOX2 ELISA Kit (AB245707)

Sandwich ELISA - Human SOX2 ELISA Kit (AB245707)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (1)

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Proteins & Peptides

Product promise