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AB220654

Human SPARC ELISA Kit

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(8 Publications)

Human SPARC ELISA Kit is a single-wash 90-min Simplestep used to quantify Human SPARC with a sensitivity of 115 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 5 citations

View Alternative Names

ON, SPARC, Basement-membrane protein 40, Osteonectin, Secreted protein acidic and rich in cysteine, BM-40

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ELISA - Human SPARC ELISA Kit (AB220654)
  • ELISA

Supplier Data

ELISA - Human SPARC ELISA Kit (AB220654)
Sandwich ELISA - Human SPARC ELISA Kit (AB220654)
  • sELISA

Supplier Data

Sandwich ELISA - Human SPARC ELISA Kit (AB220654)

Example of human SPARC standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human SPARC ELISA Kit (AB220654)
  • sELISA

Supplier Data

Sandwich ELISA - Human SPARC ELISA Kit (AB220654)

Interpolated concentrations of native SPARC in human serum and plasma samples.

The concentration of SPARC was measured in duplicate, interpolated from the SPARC standard curve and corrected for sample dilution. Undiluted samples are as follows : serum 10%, plasma (citrate) 20%, plasma (EDTA) 10% and plasma (heparin) 5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean SPARC concentration was determined to be 68.59 ng/mL in neat serum, 16.69 ng/mL in neat plasma (citrate), 71.59 ng/mL in neat plasma (EDTA) and 90.38 ng/mL in neat plasma (heparin).

Sandwich ELISA - Human SPARC ELISA Kit (AB220654)
  • sELISA

Supplier Data

Sandwich ELISA - Human SPARC ELISA Kit (AB220654)

Interpolated concentrations of native SPARC in human serum samples.

Serum from ten individual healthy human male donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean SPARC concentration was determined to be 32.4 ng/mL with a range of 16.78 – 64.84 ng/mL.

Sandwich ELISA - Human SPARC ELISA Kit (AB220654)
  • sELISA

Supplier Data

Sandwich ELISA - Human SPARC ELISA Kit (AB220654)

Interpolated concentrations of native SPARC in human plasma samples.

Regular and platelet-poor plasma samples were collected per the instructions in the protocol booklet with the matching regular and platelet-poor samples collected at the same time from the same donor. The concentrations of SPARC were measured in duplicates, interpolated from the SPARC standard curves and corrected for sample dilution. Undiluted samples are as follows : plasma (citrate) and platelet-poor plasma (citrate) 20%, plasma (EDTA) and platelet-poor plasma (EDTA) 10%, and plasma (heparin) and platelet-poor plasma heparin 5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean SPARC concentration was determined to be 14.79 ng/mL in neat plasma (citrate), 18.95 ng/mL in neat platelet-poor plasma (citrate), 71.56 ng/mL in neat plasma (EDTA), 78.24 ng/mL in neat platelet-poor plasma (EDTA), 119.66 ng/mL in neat plasma (heparin), and 102.81 ng/mL in neat platelet-poor plasma (heparin).

Key facts

Detection method

Colorimetric

Sample types

Cell culture media, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 115 pg/mL

Range

125 - 8000 pg/mL

Assay time

1h 30m

Assay Platform

Microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human SPARC ELISA Kit ab220654 is a rapid single-wash 90-min Sandwich ELISA to measure Human SPARC in cell culture media, citrate plasma, EDTA plasma, heparin plasma, serum. This SimpleStep sensitivity is 115 pg/mL.

How the assay works

Human SPARC SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human SPARC ELISA Kit ab220654 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-SPARC antibody [EPR20121] - BSA and Azide free (Capture) ab242936
- Anti-SPARC antibody [EPR20121-14] - BSA and Azide free (Detector) ab242936

SPARC is a 286-amino acid, 43 kDa protein with two calcium binding sites as well as the ability to bind copper, collagen, albumin, and cell membranes. Fibroblasts, capillary endothelial cells, platelets, macrophages, and adipocytes all produce SPARC protein. Additionally, SPARC is involved in cell proliferation, repair of tissue damage, collagen matrix formation, and osteoblast differentiation. Importantly, because SPARC is present in platelet granules and is released upon platelet activation, to properly assess circulating levels of SPARC in plasma it is recommended to prepare and analyze platelet-poor plasma samples. Mouse, rat, and bovine SPARC are 92.4%, 92.4%, and 99.0% identical to human SPARC, respectively.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.5" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.3" } ]
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Recovery

[ { "sample": "Serum", "range": "115 - 130 %", "average": "= 125" }, { "sample": "EDTA Plasma", "range": "115 - 132 %", "average": "= 124" }, { "sample": "Cell culture media", "range": "83 - 91 %", "average": "= 86" }, { "sample": "Heparin Plasma", "range": "119 - 131 %", "average": "= 126" }, { "sample": "Citrate plasma", "range": "110 - 114 %", "average": "= 113" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SPARC also known as Secreted Protein Acidic and Rich in Cysteine or osteonectin is a glycoprotein with a molecular mass of approximately 32 to 43 kDa. It is widely expressed in various tissues notably within the bone skin and extracellular matrix. SPARC influences cell-matrix interactions and modulates cellular functions such as proliferation and migration. The protein plays a role in tissue remodeling and wound healing by interacting with structural components of the matrix and regulating cell adhesion.
Biological function summary

SPARC impacts processes related to cell communication and matrix dynamics. It does not form part of large complexes but functions through interactions with matrix molecules and receptors. SPARC regulates collagen fibrillogenesis and influences the bioavailability of growth factors. It further affects angiogenesis through its ability to alter cellular adhesion and spreading which impacts vascular development and repair processes.

Pathways

SPARC integrates into regulatory cascades such as the WNT signaling and TGF-? pathways. These pathways are essential for cellular growth repair and differentiation. In the context of the TGF-? signaling pathway SPARC modulates interactions with proteins like integrins and collagens impacting fibrotic processes and matrix assembly.

Alterations in SPARC expression show associations with cancer and fibrosis. The protein functions in tumor progression where it can influence tumor cell interactions with the stroma and affect cell migration and invasion. Furthermore in fibrotic diseases SPARC regulates the deposition and organization of collagen contributing to tissue stiffening. Connections are evident between SPARC and other matrix proteins like fibronectin known for their roles in these pathological conditions.
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Product protocols

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Target data

Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity.
See full target information SPARC
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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 25: PubMed39518935

2024

Proteomic Profiling of Endothelial Cell Secretomes After Exposure to Calciprotein Particles Reveals Downregulation of Basement Membrane Assembly and Increased Release of Soluble CD59.

Applications

Unspecified application

Species

Unspecified reactive species

Alexander Stepanov,Daria Shishkova,Victoria Markova,Yulia Markova,Alexey Frolov,Anastasia Lazebnaya,Karina Oshchepkova,Daria Perepletchikova,Daria Smirnova,Liubov Basovich,Egor Repkin,Anton Kutikhin

Clinical proteomics 21:61 PubMed39487396

2024

Serum proteomics for the identification of biomarkers to flag predilection of COVID19 patients to various organ morbidities.

Applications

Unspecified application

Species

Unspecified reactive species

Madhan Vishal Rajan,Vipra Sharma,Neelam Upadhyay,Ananya Murali,Sabyasachi Bandyopadhyay,Gururao Hariprasad

Biomedicines 11: PubMed37371758

2023

Apabetalone Downregulates Fibrotic, Inflammatory and Calcific Processes in Renal Mesangial Cells and Patients with Renal Impairment.

Applications

Unspecified application

Species

Unspecified reactive species

Dean Gilham,Sylwia Wasiak,Brooke D Rakai,Li Fu,Laura M Tsujikawa,Christopher D Sarsons,Agostina Carestia,Kenneth Lebioda,Jan O Johansson,Michael Sweeney,Kamyar Kalantar-Zadeh,Ewelina Kulikowski

EBioMedicine 75:103802 PubMed34990893

2022

Blood levels of adiponectin and IL-1Ra distinguish type 3c from type 2 diabetes: Implications for earlier pancreatic cancer detection in new-onset diabetes.

Applications

Unspecified application

Species

Unspecified reactive species

Lucy Oldfield,Anthony Evans,Rohith Gopala Rao,Claire Jenkinson,Tejpal Purewal,Eftychia E Psarelli,Usha Menon,John F Timms,Stephen P Pereira,Paula Ghaneh,William Greenhalf,Christopher Halloran,Eithne Costello

Scientific reports 11:8632 PubMed33883602

2021

Prediction of sarcopenia using a battery of circulating biomarkers.

Applications

Unspecified application

Species

Unspecified reactive species

Rizwan Qaisar,Asima Karim,Tahir Muhammad,Islam Shah,Javaidullah Khan

Cancers 12: PubMed32512862

2020

Effect of Serum SPARC Levels on Survival in Patients with Digestive Tract Cancer: A Post Hoc Analysis of the AMATERASU Randomized Clinical Trial.

Applications

Unspecified application

Species

Unspecified reactive species

Taisuke Akutsu,Eisaku Ito,Mitsuo Narita,Hironori Ohdaira,Yutaka Suzuki,Mitsuyoshi Urashima

Biomolecules 10: PubMed32121498

2020

Salivary Osteocalcin as Potential Diagnostic Marker of Periodontal Bone Destruction among Smokers.

Applications

Unspecified application

Species

Unspecified reactive species

Betsy Joseph,Mukhatar Ahmed Javali,Mohasin Abdul Khader,Saad M AlQahtani,Mohammed Amanullah

Journal of dental sciences 14:269-276 PubMed31534637

2019

Diagnostic accuracy of salivary biomarkers of bone turnover in identifying patients with periodontitis in a Saudi Arabian population.

Applications

Unspecified application

Species

Unspecified reactive species

Joseph Betsy,Javali Mukhatar Ahmed,Abdul Khader Mohasin,Amanullah Mohammed,AlQahtani Nabeeh A
View all publications
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