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Human SPARCL1 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human SPARCL1 in Human in Cell culture supernatant, Serum samples.

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Sandwich ELISA - Human SPARCL1 ELISA Kit (AB213826), expandable thumbnail

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Key facts

Detection method
Colorimetric
Sample types
Cell culture supernatant, Serum
Assay type
Sandwich (quantitative)
Reactive species
Human
Range
46.9 - 3000 pg/mL
Assay time
3h 30m
Sensitivity
< 10 pg/mL

Reactivity data

Application
sELISA
Reactivity
Reacts
Dilution info
-
Notes

-

Target data

Alternative names

SPARC-like protein 1, High endothelial venule protein, MAST 9, Hevin, SPARCL1

What's included?

1 x 96 Tests
Components
ABC Diluent Buffer
1 x 12 mL
Adhesive Plate Seal
4 x 1 Unit
Anti-Human SPARCL1 coated Microplate (12 x 8 wells)
1 x 1 Unit
Antibody Diluent Buffer
1 x 12 mL
Avidin-Biotin-Peroxidase Complex (ABC)
1 x 100 µL
Biotinylated anti-Human SPARCL1 antibody
1 x 100 µL
Lyophilized recombinant Human SPARCL1 standard
2 x 1 Vial
Sample Diluent Buffer
1 x 30 mL
TMB Color Developing Agent
1 x 10 mL
TMB Stop Solution
1 x 10 mL
Wash Buffer (25X)
1 x 20 mL

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Human SPARCL1 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human SPARCL1 in Human in Cell culture supernatant, Serum samples.

Key facts

Detection method
Colorimetric
Sample types
Cell culture supernatant, Serum
Assay type
Sandwich (quantitative)
Reactive species
Human
Range
46.9 - 3000 pg/mL
Assay time
3h 30m
Assay Platform
Pre-coated microplate (12 x 8 well strips)
Sensitivity
< 10 pg/mL

Precision

Intra assay

Sample
Sample 3
n
16
mean
1261 pg/mL
SD
79.44
C.V.
= 6.7
Sample
Sample 1
n
16
mean
81 pg/mL
SD
4.45
C.V.
= 5.5
Sample
Sample 2
n
16
mean
322 pg/mL
SD
17.71
C.V.
= 5.5

Inter assay

Sample
Sample 2
n
24
mean
330 pg/mL
SD
22.11
C.V.
= 6.7
Sample
Sample 1
n
24
mean
78 pg/mL
SD
4.75
C.V.
= 6.1
Sample
Sample 3
n
24
mean
1329 pg/mL
SD
94.35
C.V.
= 7.1

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Notes

The Human SPARCL1 Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213826) is designed for the quantitative measurement of Human SPARCL1 in cell culture supernatants and serum.

The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A monoclonal antibody from mouse specific for SPARCL1 has been pre-coated onto 96-well plates. Standards (Expression system for standard: NSO; Immunogen sequence: I17-F66) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for SPARCL1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human SPARCL1 amount of sample captured in plate.

SPARCL1 (SPARC-Like Protein 1), also known as HEVIN, is a protein that in humans is encoded by the SPARCL1 gene. The cells in high endothelial venules (HEVs) in lymphoid tissues have a plump, almost cuboidal, appearance and support high levels of lymphocyte extravasation from blood, possibly due to the presence of desmosome-like junctions rather than tight junctions in the HEVs. In chronic inflammation, the activated endothelium of non-lymphoid tissues acquires an HEV-like morphology and function. SPARCL1 is highly expressed in HEV and is thought to contribute to the induction or maintenance of features of the HEV endothelium that facilitate lymphocyte migration.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

SPARCL1 also known as Hevin or Secreted Protein Acidic and Rich in Cysteine-Like 1 is a protein with a mass of approximately 75 kDa. It is part of the SPARC (Secreted Protein Acidic and Rich in Cysteine) family of matricellular proteins. SPARCL1 is mainly expressed in various tissues including the heart brain lungs and skeletal muscle. This protein exhibits strong conservation across species hinting at its importance to fundamental biological processes.

Biological function summary

SPARCL1 regulates cell-matrix interactions. It is not part of a protein complex but it interacts with other extracellular matrix components to influence cell adhesion and migration. SPARCL1 plays a role in normal homeostasis and is involved in the control of the extracellular matrix organization. It affects cellular behavior by modulating the activity of matrix metalloproteinases and influences the adhesion properties of cells by binding to components even in absence of complex formation.

Pathways

SPARCL1 impacts cellular processes like tissue remodeling and inflammation. It operates within the ECM remodeling and focal adhesion pathways. In ECM remodeling related proteins such as MMPs (Matrix Metalloproteinases) interact with SPARCL1 affecting tissue integrity and repair. In the focal adhesion pathway SPARCL1 affects cell shape and movement by regulating interactions with integrins which are integral membrane proteins that communicate the extracellular matrix to the cell.

Associated diseases and disorders

SPARCL1 has associations with cancer and neuropathological diseases. Reduced expression of SPARCL1 often occurs in various cancers including prostate and colorectal cancers suggesting its potential role as a tumor suppressor. In neuropathological conditions such as Alzheimer's disease altered SPARCL1 expression affects neuroinflammation and synaptic functions related to the amyloid precursor protein (APP) which plays a critical role in Alzheimer's pathology. Understanding these pathways offers insight into therapeutic potential in these disorders.

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  • Sandwich ELISA - Human SPARCL1 ELISA Kit (ab213826), expandable thumbnail

    Sandwich ELISA - Human SPARCL1 ELISA Kit (ab213826)

    Human SPARCL1 ELISA Kit (ab213826) Standard Curve.

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Product protocols

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