AB272478

Human SPARCL1 ELISA Kit (SPARC-like protein 1)

Recombinant
SimpleStep
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(2 Publications)

Human SPARCL1 ELISA Kit (SPARC-like protein 1) is a single-wash 90-min Simplestep used to quantify Human SPARCL1 (SPARC-like protein 1) with a sensitivity of 7.89 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

SPARC-like protein 1, High endothelial venule protein, MAST 9, Hevin, SPARCL1

3 Images

Sandwich ELISA - Human SPARCL1 ELISA Kit (SPARC-like protein 1) (AB272478)

sELISA

Supplier Data

Sandwich ELISA - Human SPARCL1 ELISA Kit (SPARC-like protein 1) (AB272478)

Example of human SPARCL1 standard curve in Sample Diluent NS

sELISA

Supplier Data

Sandwich ELISA - Human SPARCL1 ELISA Kit (SPARC-like protein 1) (AB272478)

Interpolated concentrations of native SPARCL1 in human brain tissue.

based on a 25 μg/mL extract load and human lung based on a 250 μg/mL extract load. The concentrations of SPARCL1 were measured in duplicate and interpolated from the SPARCL1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean SPARCL1 concentration was determined to be 519.79 pg/mL in brain extract and 108.09 pg/mL in lung extract.

sELISA

Supplier Data

Sandwich ELISA - Human SPARCL1 ELISA Kit (SPARC-like protein 1) (AB272478)

Interpolated concentrations of native SPARCL1 in human serum and plasma samples.

The concentrations of SPARCL1 were measured in duplicates, interpolated from the SPARCL1 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 100, plasma (citrate) 1 : 200, plasma (EDTA) 1 : 100, and plasma (heparin) 1 : 200. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean SPARCL1 concentration was determined to be 215.28 ng/mL in serum, 468.22 ng/mL in plasma (citrate), 231.62 ng/mL in plasma (EDTA), and 428.58 ng/mL in plasma (heparin).

Key facts

Detection method

Colorimetric

Sample types

Tissue Extracts, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 7.89 pg/mL

Range

39.06 - 2500 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human SPARCL1 ELISA kit (ab272478) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human SPARCL1 protein in serum, plasma and cell and tissue culture extract. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human SPARCL1 with 7.89 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less

-High sensitivity, specificity and reproducibility from superior antibodies

-Fully validated in biological samples

-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

SPARCL1 (SPARC-Like Protein 1), also known as HEVIN, is a 75 kD protein that is encoded by the SPARCL1 gene. SPARCL1 is highly expressed in high endothelial venules (HEVs) and is thought to contribute to the induction or maintenance of features of the HEV endothelium that facilitate lymphocyte migration. SPARCL1 is also highly expressed in heart, lung, brain, skeletal muscle, ovary, colon, and small intestine tissues. SPARCL1 may have implications in the regulation of cell adhesion, migration and proliferation. The antibody pair was designed against the mature full-length extracellular fragment of SPARCL1. SPARCL1 shares 64%, 64%, 72%, and 93% sequence homology in mouse, rat, cow, and monkey, respectively.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.27" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.3" } ]

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Recovery

[ { "sample": "Serum", "range": "89 - 95 %", "average": "= 92" }, { "sample": "EDTA Plasma", "range": "96 - 104 %", "average": "= 99" }, { "sample": "Tissue Extracts", "range": "104 - 106 %", "average": "= 105" }, { "sample": "Heparin Plasma", "range": "93 - 108 %", "average": "= 99" }, { "sample": "Citrate plasma", "range": "92 - 116 %", "average": "= 102" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SPARCL1 also known as Hevin or Secreted Protein Acidic and Rich in Cysteine-Like 1 is a protein with a mass of approximately 75 kDa. It is part of the SPARC (Secreted Protein Acidic and Rich in Cysteine) family of matricellular proteins. SPARCL1 is mainly expressed in various tissues including the heart brain lungs and skeletal muscle. This protein exhibits strong conservation across species hinting at its importance to fundamental biological processes.

Biological function summary

SPARCL1 regulates cell-matrix interactions. It is not part of a protein complex but it interacts with other extracellular matrix components to influence cell adhesion and migration. SPARCL1 plays a role in normal homeostasis and is involved in the control of the extracellular matrix organization. It affects cellular behavior by modulating the activity of matrix metalloproteinases and influences the adhesion properties of cells by binding to components even in absence of complex formation.

Pathways

SPARCL1 impacts cellular processes like tissue remodeling and inflammation. It operates within the ECM remodeling and focal adhesion pathways. In ECM remodeling related proteins such as MMPs (Matrix Metalloproteinases) interact with SPARCL1 affecting tissue integrity and repair. In the focal adhesion pathway SPARCL1 affects cell shape and movement by regulating interactions with integrins which are integral membrane proteins that communicate the extracellular matrix to the cell.

SPARCL1 has associations with cancer and neuropathological diseases. Reduced expression of SPARCL1 often occurs in various cancers including prostate and colorectal cancers suggesting its potential role as a tumor suppressor. In neuropathological conditions such as Alzheimer's disease altered SPARCL1 expression affects neuroinflammation and synaptic functions related to the amyloid precursor protein (APP) which plays a critical role in Alzheimer's pathology. Understanding these pathways offers insight into therapeutic potential in these disorders.

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Product protocols

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Target data

See full target information SPARC-like protein 1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 14:18148 PubMed39103428

2024

Urine biomarkers can predict prostate cancer and PI-RADS score prior to biopsy.

Applications

Unspecified application

Species

Unspecified reactive species

Blaz Pavlovic,Konstantin Bräutigam,Florence Dartiguenave,Paul Martel,Arnas Rakauskas,Valérie Cesson,Markus Veit,Pascal Oechslin,Alexander Gu,Thomas Hermanns,Karim Saba,Cédric Poyet,Andreas M Hötker,Niels J Rupp,Massimo Valerio,Laurent Derré,Daniel Eberli,Irina Banzola

Neuropsychiatric disease and treatment 19:2709-2728 PubMed38077240

2023

Serum Secreted Protein Acidic and Rich in Cysteine-Like 1 as a Biochemical Predictor for Prognosticating Clinical Outcomes After Acute Supratentorial Intracerebral Hemorrhage: A Prospective Cohort Study.

Applications

Unspecified application

Species

Unspecified reactive species

Jianjun Huang,Fangping Shao,Bin Chen,Guanrong Zheng,Jia Shen,Shenzhong Qiu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human SPARCL1 ELISA Kit (SPARC-like protein 1)

Sandwich ELISA - Human SPARCL1 ELISA Kit (SPARC-like protein 1) (AB272478)

Sandwich ELISA - Human SPARCL1 ELISA Kit (SPARC-like protein 1) (AB272478)

Sandwich ELISA - Human SPARCL1 ELISA Kit (SPARC-like protein 1) (AB272478)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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