Human SQSTM1/p62 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human SQSTM1/p62 with a sensitivity of 100.98 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
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- Cells 12:2023Autophagy Receptor p62 Regulates SARS-CoV-2-Induced Inflammation in COVID-19.Applications:Unspecified applicationReactive species:Unspecified reactive speciesVerica Paunovic,Ljubica Vucicevic,Maja Misirkic Marjanovic,Vladimir Perovic,Biljana Ristic,Mihajlo Bosnjak,Milos Mandic,Danijela Stevanovic,Ljubica Harhaji-Trajkovic,Jovan Lalosevic,Milos Nikolic,Branka Bonaci-Nikolic,Vladimir TrajkovicPubMed 37174682
- American journal of translational research 14:6056-60662022Changes of serum P62 and Beclin 1 in patients with pulmonary embolism during treatment and their predictive value for efficacy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXuehui Zhang,Liu Huang,Lijiang Shao,Xuxia Chen,Jinguo Chu,Xueqin ChenPubMed 36247304
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell Lysate
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 265.625 - 17000 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 100.98 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
The protein expressed by the SQSTM1 gene is an autophagy receptor essential for selective macroautophagy (aggrephagy). It acts as a bridge between polyubiquitinated cargo and autophagosomes, interacting with both the cargo and an autophagy modifier from the MAP1 LC3 family. Alongside WDFY3, it plays a role in forming and autophagically degrading cytoplasmic ubiquitin-containing inclusions and recruiting ubiquitinated proteins to nuclear PML bodies. SQSTM1 may regulate NFKB1 activation by TNF-alpha, nerve growth factor (NGF), and interleukin-1, and may influence titin/TTN signaling in muscle cells. It may also affect signaling cascades via ubiquitination and be involved in cell differentiation, apoptosis, immune response, and regulation of potassium channels. The protein is involved in endosome organization by retaining vesicles in the perinuclear cloud; ubiquitination by RNF26 allows it to attract vesicle-associated adapters, forming a molecular bridge to organize endosomal pathways. Additionally, it promotes the relocalization of Lys-63-linked ubiquitinated STING1 to autophagosomes and acts as an activator of the NFE2L2/NRF2 pathway by interacting with KEAP1, which inactivates the BCR(KEAP1) complex, resulting in nuclear accumulation of NFE2L2/NRF2 and expression of cytoprotective genes. This supplementary information is collated from multiple sources and compiled automatically.
Alternative names
ORCA, OSIL, SQSTM1, Sequestosome-1, EBI3-associated protein of 60 kDa, Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa, Ubiquitin-binding protein p62, EBIAP, p60, p62
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Human SQSTM1/p62 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human SQSTM1/p62 with a sensitivity of 100.98 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell Lysate
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 265.625 - 17000 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 100.98 pg/mL
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Extract | n 8 | C.V. 3.2 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Extract | n 3 | C.V. 5.66 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell Lysate | Average % = 95 | Range 87 - 101 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human SQSTM1/p62 ELISA Kit (ab289654) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human SQSTM1/p62 in cell extract samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human SQSTM1/p62 with 100.98 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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3 product images
Sandwich ELISA - Human SQSTM1/p62 ELISA Kit (ab289654)
Interpolated concentrations of SQSTM1/p62 in human cell extracts.
Interpolated concentration of native SQSTM1/p62 was measured in duplicate in human cell extracts based on a 5 μg/mL extract load. The interpolated values are plotted (mean +/- SD, n=2). Human SQSTM1/p62 concentrations were undetectable in Daudi and Jurkat cell extracts. Sample dilutions are made in 1X Cell Extraction Buffer PTR.
Sandwich ELISA - Human SQSTM1/p62 ELISA Kit (ab289654)
Interpolated concentrations of Human SQSTM1/p62 in Hela cell extract.
Interpolated concentration of native SQSTM1/p62 was measured in duplicate at different sample concentrations. Undiluted samples are 10 μg/mL Hela cell extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.
Sandwich ELISA - Human SQSTM1/p62 ELISA Kit (ab289654)
Example of Human SQSTM1/p62 standard curve in 1X Cell Extraction Buffer PTR.
Example of Human SQSTM1/p62 standard curve. Background-subtracted data values (mean +/- SD) are graphed.
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Product protocols
- Visit the general protocols
- Visit troubleshooting
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