AB289654

Human SQSTM1/p62 ELISA Kit

Recombinant
SimpleStep
What is this?

Be the first to review this product! Submit a review

(2 Publications)

Human SQSTM1/p62 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human SQSTM1/p62 with a sensitivity of 100.98 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

ORCA, OSIL, SQSTM1, Sequestosome-1, EBI3-associated protein of 60 kDa, Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa, Ubiquitin-binding protein p62, EBIAP, p60, p62

3 Images

Sandwich ELISA - Human SQSTM1/p62 ELISA Kit (AB289654)

sELISA

Supplier Data

Sandwich ELISA - Human SQSTM1/p62 ELISA Kit (AB289654)

Interpolated concentrations of Human SQSTM1/p62 in Hela cell extract.

Interpolated concentration of native SQSTM1/p62 was measured in duplicate at different sample concentrations. Undiluted samples are 10 μg/mL Hela cell extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

sELISA

Supplier Data

Sandwich ELISA - Human SQSTM1/p62 ELISA Kit (AB289654)

Interpolated concentrations of SQSTM1/p62 in human cell extracts.

Interpolated concentration of native SQSTM1/p62 was measured in duplicate in human cell extracts based on a 5 μg/mL extract load. The interpolated values are plotted (mean +/- SD, n=2). Human SQSTM1/p62 concentrations were undetectable in Daudi and Jurkat cell extracts. Sample dilutions are made in 1X Cell Extraction Buffer PTR.

sELISA

Supplier Data

Sandwich ELISA - Human SQSTM1/p62 ELISA Kit (AB289654)

Example of Human SQSTM1/p62 standard curve in 1X Cell Extraction Buffer PTR.

Example of Human SQSTM1/p62 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 100.98 pg/mL

Range

265.625 - 17000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

style

left-column

Product details

Human SQSTM1/p62 ELISA Kit (ab289654) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human SQSTM1/p62 in cell extract samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human SQSTM1/p62 with 100.98 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

style

left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.66" }, { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.2" } ]

style

left-column

Recovery

[ { "sample": "Cell Lysate", "range": "87 - 101 %", "average": "= 95" } ]

style

left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab289654-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB289654-CMP07", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB289654-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB289654-CMP12", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB289654-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB289654-CMP10", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human SQSTM1/p62 Lyophilized Recombinant Protein", "number":"AB289654-CMP02", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human SQSTM1/p62 Capture Antibody", "number":"AB289654-CMP04", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BI", "number":"AB289654-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB289654-CMP06", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human SQSTM1/p62 Detector Antibody", "number":"AB289654-CMP05", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB289654-CMP09", "productcode":"" } ] } } }

style

left-column

Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

style

left-column

style

left-column

Product protocols

style

left-column

Target data

The protein expressed by the SQSTM1 gene is an autophagy receptor essential for selective macroautophagy (aggrephagy). It acts as a bridge between polyubiquitinated cargo and autophagosomes, interacting with both the cargo and an autophagy modifier from the MAP1 LC3 family. Alongside WDFY3, it plays a role in forming and autophagically degrading cytoplasmic ubiquitin-containing inclusions and recruiting ubiquitinated proteins to nuclear PML bodies. SQSTM1 may regulate NFKB1 activation by TNF-alpha, nerve growth factor (NGF), and interleukin-1, and may influence titin/TTN signaling in muscle cells. It may also affect signaling cascades via ubiquitination and be involved in cell differentiation, apoptosis, immune response, and regulation of potassium channels. The protein is involved in endosome organization by retaining vesicles in the perinuclear cloud; ubiquitination by RNF26 allows it to attract vesicle-associated adapters, forming a molecular bridge to organize endosomal pathways. Additionally, it promotes the relocalization of Lys-63-linked ubiquitinated STING1 to autophagosomes and acts as an activator of the NFE2L2/NRF2 pathway by interacting with KEAP1, which inactivates the BCR(KEAP1) complex, resulting in nuclear accumulation of NFE2L2/NRF2 and expression of cytoprotective genes. This supplementary information is collated from multiple sources and compiled automatically.

See full target information SQSTM1

style

left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cells 12: PubMed37174682

2023

Autophagy Receptor p62 Regulates SARS-CoV-2-Induced Inflammation in COVID-19.

Applications

Unspecified application

Species

Unspecified reactive species

Verica Paunovic,Ljubica Vucicevic,Maja Misirkic Marjanovic,Vladimir Perovic,Biljana Ristic,Mihajlo Bosnjak,Milos Mandic,Danijela Stevanovic,Ljubica Harhaji-Trajkovic,Jovan Lalosevic,Milos Nikolic,Branka Bonaci-Nikolic,Vladimir Trajkovic

American journal of translational research 14:6056-6066 PubMed36247304

2022

Changes of serum P62 and Beclin 1 in patients with pulmonary embolism during treatment and their predictive value for efficacy.

Applications

Unspecified application

Species

Unspecified reactive species

Xuehui Zhang,Liu Huang,Lijiang Shao,Xuxia Chen,Jinguo Chu,Xueqin Chen

View all publications

style

left-column

style

left-column

websiteProtocolBooklet

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human SQSTM1/p62 ELISA Kit

Sandwich ELISA - Human SQSTM1/p62 ELISA Kit (AB289654)

Sandwich ELISA - Human SQSTM1/p62 ELISA Kit (AB289654)

Sandwich ELISA - Human SQSTM1/p62 ELISA Kit (AB289654)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (2)

Complementary Products

Assay Kits

Assay Kits

Primary Antibodies

Secondary Antibodies

Product promise