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AB314354

Human SREC-II ELISA Kit

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Human SREC-II ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human SREC-II in Human in Cell Culture Media, Biofluids samples.

View Alternative Names

SREC2, SREPCR, SCARF2, Scavenger receptor class F member 2, SRECRP-1, Scavenger receptor expressed by endothelial cells 2 protein, SREC-II

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Sandwich ELISA - Human SREC-II ELISA Kit (AB314354)
  • sELISA

Supplier Data

Sandwich ELISA - Human SREC-II ELISA Kit (AB314354)

Example of standard curve using ab314354. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Plasma, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 0.84 ng/mL

Range

0.84 - 200 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human SREC-II ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human SREC-II in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human SREC-II coated on a 96-well plate.

This ELISA kit shows no cross-reactivity with the following cytokines tested: human ACE, Activin RIB (ALK-4), ADAM23, Artemin, Cardiotrophin1 (CT-1), Cathepsin V, FABP1 (L-FABP), FGF-20, GDF-8 (Myostatin), HAI-1, IL-27 R alpha (WSX-1), Insulin R (CD220), Kallikrein 7, LIF R alpha, Lipocalin-1, LTbR (Lymphotoxin beta R), Mesothelin, MFRP, Neuropilin-2, Neurturin, Nidogen-2, Olfactomedin-2 (Noelin-2), p53, PDECGF (Thymidine Phosphorylase), PDGF-CC, Progranulin, Ret, ROBO4, Semaphorin 6B, Serpin F1 (PEDF), SREC-I (SCARF1), TLR1, TLR3, TPP1 (Tripeptidyl-peptidase I), TREM-2, TrkC, TROY (TNFRSF19), Uromodulin, XIAP.

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Recovery

[ { "sample": "Serum", "range": "101 - 110 %", "average": "= 107" }, { "sample": "Plasma", "range": "93 - 110 %", "average": "= 99.35" }, { "sample": "Cell culture media", "range": "98 - 126 %", "average": "= 109.7" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab314354-1x96Tests", "assetComponentDetails": [ { "size":"1 x 200 µL", "name":"150X HRP-Streptavidin", "number":"AB314354-CMP03", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB314354-CMP04", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent (Item E2)", "number":"AB314354-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human SREC-II Protein Standard", "number":"AB314354-CMP10", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human SREC-II Antibody-coated ELISA Plate", "number":"AB314354-CMP08", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB314354-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB314354-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human SREC-II Detection Antibody", "number":"AB314354-CMP09", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The SCARF2 protein also known as SREC-II (scavenger receptor class F member 2) is a cell surface glycoprotein involved in endocytosis and signaling. It has an approximate molecular weight of 92 kDa. This protein mainly expresses in tissues such as the placenta lung and liver and is also found in the skin and heart. SCARF2 binds to lipoproteins apoptotic cells and pathogens displaying a range of interactions essential for its function.
Biological function summary

Within the cellular context SCARF2 influences processes like lipid metabolism and immune response. While it functions mostly independently SCARF2 occasionally interacts with other scavenger receptors for efficient uptake of modified low-density lipoproteins. Its binding abilities connect it to cellular housekeeping and pathogen clearance roles contributing to maintenance of cellular homeostasis and defense mechanisms.

Pathways

Interactions involving SCARF2 influence lipid metabolism and immune signaling networks. It plays a role in lipid transport pathways where it potentially associates with proteins like CD36 which also bind lipoproteins. In immune pathways SCARF2 affects toll-like receptor signaling interacting indirectly with proteins such as TLR4 contributing to the modulation of inflammatory responses.

Researchers have identified connections between SCARF2 and atherosclerosis as well as skin disorders like ichthyosis. In atherosclerosis SCARF2 mediates lipoprotein uptake possibly influencing the pathogenesis alongside proteins like LOX-1. In ichthyosis mutations in SCARF2 have been observed linking it to abnormal cornification processes with impacts that potentially involve interactions with proteins like transglutaminase.
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Product protocols

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Target data

Probable adhesion protein, which mediates homophilic and heterophilic interactions. In contrast to SCARF1, it poorly mediates the binding and degradation of acetylated low density lipoprotein (Ac-LDL) (By similarity).
See full target information SCARF2
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websiteProtocolBooklet
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Product promise

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