JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB314739

Human ST14 ELISA Kit

Be the first to review this product! Submit a review

|

(0 Publication)

Human ST14 ELISA Kit is a Sandwich ELISA for the measurement of Human ST14 in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

PRSS14, SNC19, TADG15, ST14, Suppressor of tumorigenicity 14 protein, Matriptase, Membrane-type serine protease 1, Prostamin, Serine protease 14, Serine protease TADG-15, Tumor-associated differentially-expressed gene 15 protein, MT-SP1

1 Images
Sandwich ELISA - Human ST14 ELISA Kit (AB314739)
  • sELISA

Supplier Data

Sandwich ELISA - Human ST14 ELISA Kit (AB314739)

Example of standard curve using ab314739. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Serum, Plasma, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.41 ng/mL

Range

0.41 - 100 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style
left-column

Complementary Products

Primary Antibodies

AB307599

Anti-ST14 antibody [EPR26785-94]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ST14 antibody [EPR26785-94] (AB307599)

  • 0
  • 0
Proteins & Peptides

AB114597

Recombinant Human ST14 protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human ST14 protein (GST tag N-Terminus) (AB114597)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Human ST14 ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human ST14 in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human ST14 coated on a 96-well plate.

This ELISA kit shows no cross-reactivity with the following cytokines tested: human AMIGO, Aminopeptidase LRAP (ERAP2), Amnionless, Arylsulfatase A, Bcl-w, CD109, CD157, CD34, CD83, CLEC-1, CLEC10A (CD301), CMG-2, CREG, Cystatin SN, Cytokeratin-8, Dectin-1 (CLEC7A), Desmocollin-3, Endoglycan, Galectin-4, HAPLN1, Jagged 1, Langerin (CD207), Lumican, MEP1B, Nectin-3, OX40 (TNFRSF4), OX40 Ligand (TNFSF4), p27, Pappalysin-2, Plexin B3, Plexin D1, proGRP, PSA-total, Reg1B, RGM-A, ROBO2, Spinesin, TWEAK R (TNFRSF12), ULBP-3.

style
left-column

Recovery

[ { "sample": "Serum", "range": "90 - 107 %", "average": "= 98.14" }, { "sample": "Plasma", "range": "84 - 138 %", "average": "= 98.71" }, { "sample": "Cell culture media", "range": "86 - 94 %", "average": "= 90.52" } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab314739-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB314739-CMP04", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent (Item E2)", "number":"AB314739-CMP05", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB314739-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human ST14 Protein Standard", "number":"AB314739-CMP10", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human ST14 Detection Antibody", "number":"AB314739-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human ST14 Antibody-coated ELISA Plate", "number":"AB314739-CMP08", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin", "number":"AB314739-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB314739-CMP07", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ST14 also known as matriptase is a serine protease with a molecular mass of about 92 kDa. It exists as a single-chain zymogen and activates through cleavage to form a two-chain form. Matriptase resides on the surface of epithelial cells and shows high expression in several tissues such as the epidermis prostate and gastrointestinal tract. This protease plays a part in tissue homeostasis by initiating proteolytic cascades that modify the extracellular matrix and influence cellular processes.
Biological function summary

Matriptase regulates proteolytic activation events in the extracellular environment. It instigates cascades that activate specific growth factors and proteases playing a role in cell proliferation and migration. Matriptase forms a complex with hepatocyte growth factor activator inhibitor-1 (HAI-1) which modulates its activity. The balance between matriptase and its inhibitors is important for controlling epithelial cell dynamics and maintaining a healthy cellular environment.

Pathways

Matriptase contributes to key signaling pathways like the epidermal growth factor receptor (EGFR) pathway and the plasminogen activation pathway. Within the EGFR signaling matriptase activates growth factors that influence cell proliferation and survival. It also intersects with proteins like epithelial growth factor receptor and plasminogen which are significant for cellular functions and homeostasis. These pathways are important for maintaining normal tissue function and responding to cellular stress.

Deregulation of matriptase is linked to conditions like cancer and ichthyosis. Matriptase overexpression or aberrant activity often relates to tumor progression and cancer metastasis particularly in breast and prostate cancer. Dysregulation also associates with the skin disorder ichthyosis due to imbalance with its inhibitor HAI-1 leading to defective epidermal permeability. Understanding matriptase's role in these diseases helps in designing therapeutic strategies and targeting the protease with specific inhibitors.
style
left-column

Product protocols

style
left-column

Target data

Exhibits trypsin-like activity as defined by cleavage of synthetic substrates with Arg or Lys as the P1 site (PubMed : 10373424). Involved in the terminal differentiation of keratinocytes through prostasin (PRSS8) activation and filaggrin (FLG) processing (PubMed : 18843291). Proteolytically cleaves and therefore activates TMPRSS13 (PubMed : 28710277).
See full target information ST14
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com