Human ST2 ELISA Kit (IL1RL1) is a single-wash 90-min Simplestep used to quantify Human ST2 (IL1RL1) with a sensitivity of 22.06 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
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- International journal of hematology 116:937-9462022Plasma biomarkers associated with survival and thrombosis in hospitalized COVID-19 patients.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDavid Cabrera-Garcia,Andrea Miltiades,Peter Yim,Samantha Parsons,Katerina Elisman,Mohammad Taghi Mansouri,Gebhard Wagener,Neil L HarrisonPubMed 35994163
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture media, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 109.38 - 7000 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 22.06 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Receptor for interleukin-33 (IL-33) which plays crucial roles in innate and adaptive immunity, contributing to tissue homeostasis and responses to environmental stresses together with coreceptor IL1RAP (PubMed:35238669). Its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8. Possibly involved in helper T-cell function (Probable) (PubMed:16286016). Upon tissue injury, induces UCP2-dependent mitochondrial rewiring that attenuates the generation of reactive oxygen species and preserves the integrity of Krebs cycle required for persistent production of itaconate and subsequent GATA3-dependent differentiation of inflammation-resolving alternatively activated macrophages (By similarity). Isoform B. Inhibits IL-33 signaling.
Alternative names
DER4, ST2, T1, IL1RL1, Interleukin-1 receptor-like 1, Protein ST2
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Human ST2 ELISA Kit (IL1RL1) is a single-wash 90-min Simplestep used to quantify Human ST2 (IL1RL1) with a sensitivity of 22.06 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture media, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 109.38 - 7000 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 22.06 pg/mL
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Serum | n 8 | C.V. 3.2 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Serum | n 3 | C.V. 4.4 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 114 | Range 108 - 118 % |
Sample type Serum | Average % = 108 | Range 98 - 118 % |
Sample type EDTA Plasma | Average % = 107 | Range 82 - 118 % |
Sample type Cell culture media | Average % = 87 | Range 81 - 94 % |
Sample type Heparin Plasma | Average % = 89 | Range 81 - 96 % |
Sample type Citrate plasma | Average % = 98 | Range 84 - 108 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human ST2 ELISA Kit (IL1RL1) (ab254505) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of ST2 (IL1RL1) protein in cell culture supernatant, cit plasma, hep plasma, serum, edta plasma, and cell culture media. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human ST2 (IL1RL1) with 22.06 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
ST2, also known as or Interleukin 1 receptor-like 1 (IL-1RL1), is a protein encoded by the IL1RL1 gene. ST2 gene is a member of the interleukin 1 receptor family. ST2, interleukin 1 receptor, type II and interleukin 1 receptor like 2 form a cytokine receptor gene cluster in a region mapped to chromosome 2q12. Studies of the similar gene in mouse reported protein induction by pro-inflammatory stimuli with involvement in the function of helper T cells. ST2 is necessary for endotoxin tolerance by inhibiting TLR responses inducing the Th2 response. Human ST2 shares 68%, 64%, 66%, and 96% sequence homology with mouse, rat, bovine, and monkey, respectively.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ST2 also known as IL-1RL1 or IL-1RL1 chimera protein is a member of the IL-1 receptor family. It has a molecular mass of approximately 55 kDa. This protein exists in different forms including a membrane-bound form and a soluble variant. Researchers typically find ST2 expressed in various tissues including the lungs gut and cardiovascular system. The expression level can vary significantly depending on the physiological or pathological state.
- Biological function summary
ST2 interacts with interleukin-33 (IL-33) and functions as its receptor. The binding of IL-33 to ST2 activates immune responses particularly influencing T-helper 2 (Th2) cells. ST2 forms part of a receptor complex including IL-1 receptor accessory protein IL-1RAcP necessary for signal transduction. This interaction leads to the activation of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB) and mitogen-activated protein kinase (MAPK) signaling pathways facilitating inflammatory responses.
- Pathways
IL-33 signals through the ST2 receptor participating actively in the immune signaling pathway. This pathway plays a significant role in modulating inflammatory and immune responses. ST2 association with NF-kB broadly affects inflammatory signaling while its involvement with the MAPK pathway aids in cellular responses to cytokines and stress. The close relationship with the IL-33/ST2 axis highlights its importance in maintaining immune homeostasis and reacting to environmental stressors.
- Associated diseases and disorders
ST2 has significant associations with asthma and cardiovascular diseases. In asthma the IL-33/ST2 axis critically contributes to airway inflammation and hyperresponsiveness. Similarly in the context of cardiovascular diseases elevated levels of soluble ST2 are markers of heart failure with the ST2/IL-33 axis contributing to cardiac remodeling. ST2's connection with inflammatory processes implies it also interacts with other cytokines such as interleukin-6 (IL-6) exacerbating the disease states through shared pathways.
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5 product images
Sandwich ELISA - Human ST2 ELISA Kit (IL1RL1) (ab254505)
Example of human ST2 standard curve in Sample Diluent NS.
The ST2 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human ST2 ELISA Kit (IL1RL1) (ab254505)
Interpolated concentrations of native ST2 in human serum and plasma samples.
The concentrations of ST2 were measured in duplicates, interpolated from the ST2 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 50%, plasma (EDTA) 50% and plasma (heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ST2 concentration was determined to be 13.86 ng/mL in serum, 9.81 ng/mL in plasma (citrate), 12.62 ng/mL in plasma (EDTA), and 9.06 ng/mL in plasma (Heparin).
Sandwich ELISA - Human ST2 ELISA Kit (IL1RL1) (ab254505)
Interpolated concentrations of native ST2 in human cell culture supernatant samples.
The concentrations of ST2 were measured in duplicates, interpolated from the ST2 standard curves and corrected for sample dilution. Undiluted samples are as follows: HUVEC cell culture supernatant 5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ST2 concentration was determined to be 68.42 ng/mL in HUVEC cell culture supernatant.
Sandwich ELISA - Human ST2 ELISA Kit (IL1RL1) (ab254505)
10% Serum from ten individual healthy human female donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ST2 concentration was determined to be 10.13 ng/mL with a range of 5.18 – 15.85 ng/mL.
Sandwich ELISA - Human ST2 ELISA Kit (IL1RL1) (ab254505)
10% Serum from ten individual healthy human male donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ST2 concentration was determined to be 12.42 ng/mL with a range of 7.16 – 20.19 ng/mL.
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