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AB314355

Human ST3Gal1 ELISA Kit

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Human ST3Gal1 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human ST3Gal1 in Human in Cell Culture Media, Biofluids samples.

View Alternative Names

SIAT4, SIAT4A, ST3GAL1, Gal-NAc6S, Monosialoganglioside sialyltransferase, SIATFL, ST3Gal I, ST3GalA.1, ST3O, Sialyltransferase 4A, ST3GalI, SIAT4-A

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Sandwich ELISA - Human ST3Gal1 ELISA Kit (AB314355)
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Supplier Data

Sandwich ELISA - Human ST3Gal1 ELISA Kit (AB314355)

Example of standard curve using ab314355. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Plasma, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 1.8 ng/mL

Range

1.8 - 400 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human ST3Gal1 ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human ST3Gal1 in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human ST3Gal1 coated on a 96-well plate.

This ELISA kit shows no cross-reactivity with the following cytokines tested: human C1qTNF9, Carbonic Anhydrase VA, CANT1, Cathepsin H, Contactin-5, Chymotrypsin C/CTRC, Draxin, EphB2, EphB3, FABP8, Fgr, FKBP51, FUCA1, Galanin, GALNT10, Gastrokine 1, Glyoxalase II, HS3ST1, HS3ST3B1, Lin28, Lysyl Oxidase Homolog, LRRTM4, Methionine Aminopeptidase 1D, Matrilin-2, MCEMP1, Mcl-1, MDGA2, MEF2C, Methionine Aminopeptidase 2, Neurocan, Nogo-A, PCK1, PlGF-2, PON1, SALM4/LRFN3, Semaphorin 6C, SorCS2, ST8SIA1, Tsukushi/TSK.

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Recovery

[ { "sample": "Serum", "range": "72 - 95 %", "average": "= 83" }, { "sample": "Plasma", "range": "69 - 121 %", "average": "= 91.58" }, { "sample": "Cell culture media", "range": "91 - 127 %", "average": "= 110" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ST3Gal I also known as ST3 beta-galactoside alpha-23-sialyltransferase 1 or sialyltransferase 1 is an enzyme with a molecular mass of approximately 35 kDa. This enzyme catalyzes the transfer of sialic acid from CMP-sialic acid to galactose-containing substrates specifically creating the sialyl T antigen. ST3Gal I is expressed in many tissues including the gastrointestinal tract lungs and the immune system reflecting its diverse roles in different physiological contexts.
Biological function summary

ST3Gal I participates in glycosylation processes that modify proteins and lipids influencing cell-cell interactions immune responses and protein stability. It often associates with glycosylation complexes ensuring the proper addition of sialic acid to glycoconjugates. These modifications are critical for the formation of sialyated glycoconjugates which play significant roles in cellular communication and pathogen recognition.

Pathways

ST3Gal I impacts the sialylation pathway and by extension affects glycan biosynthesis pathways. It modifies glycoproteins and glycolipids which are important in cell adhesion and signaling pathways. Within these pathways ST3Gal I shows interactions with proteins involved in these processes such as those in the selectin family which mediate leukocyte trafficking.

Altered ST3Gal I expression or activity links to certain cancers and immune disorders. Its dysregulation can lead to aberrant glycosylation patterns impacting processes like tumor metastasis and immune evasion. For instance the sialylation of mucins which influences tumor progression requires ST3Gal I. Also its interaction with other glycosyltransferases includes the regulation of immune cell surface antigens connecting it to autoimmune conditions.
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Product protocols

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Target data

A beta-galactoside alpha2-3 sialyltransferase involved in terminal sialylation of glycoproteins and glycolipids (PubMed : 31784620, PubMed : 8027041). Catalyzes the transfer of sialic acid (N-acetyl-neuraminic acid; Neu5Ac) from the nucleotide sugar donor CMP-Neu5Ac onto acceptor Galbeta-(1->3)-GalNAc-terminated glycoconjugates through an alpha2-3 linkage (PubMed : 31784620, PubMed : 8027041). Adds sialic acid to the core 1 O-glycan, Galbeta-(1->3)-GalNAc-O-Ser/Thr, which is a major structure of mucin-type O-glycans. As part of a homeostatic mechanism that regulates CD8-positive T cell numbers, sialylates core 1 O-glycans of T cell glycoproteins, SPN/CD43 and PTPRC/CD45. Prevents premature apoptosis of thymic CD8-positive T cells prior to peripheral emigration, whereas in the secondary lymphoid organs controls the survival of CD8-positive memory T cells generated following a successful immune response (By similarity). Transfers sialic acid to asialofetuin, presumably onto Galbeta-(1->3)-GalNAc-O-Ser (By similarity). Sialylates GM1a, GA1 and GD1b gangliosides to form GD1a, GM1b and GT1b, respectively (By similarity) (PubMed : 8027041).
See full target information ST3GAL1
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Complementary Products

Primary Antibodies

AB96129

Anti-ST3Gal I antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ST3Gal I antibody (AB96129)

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