Human Stabilin-1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Stabilin-1 with a sensitivity of 33.574 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Serum, EDTA Plasma, Cell culture supernatant, Citrate plasma, Heparin Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 171.875 - 11000 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 33.574 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Acts as a scavenger receptor for acetylated low density lipoprotein. Binds to both Gram-positive and Gram-negative bacteria and may play a role in defense against bacterial infection. When inhibited in endothelial tube formation assays, there is a marked decrease in cell-cell interactions, suggesting a role in angiogenesis. Involved in the delivery of newly synthesized CHID1/SI-CLP from the biosynthetic compartment to the endosomal/lysosomal system.
Alternative names
FEEL1, KIAA0246, STAB1, Stabilin-1, MS-1 antigen, FEEL-1
What's included?
Recommended products
Human Stabilin-1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Stabilin-1 with a sensitivity of 33.574 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Serum, EDTA Plasma, Cell culture supernatant, Citrate plasma, Heparin Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 171.875 - 11000 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 33.574 pg/mL
- General recovery
- 114%
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Serum | n 8 | C.V. 8.3 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Serum | n 3 | C.V. 8.4 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 114 | Range 109 - 120 % |
Sample type Citrate plasma | Average % = 117 | Range 114 - 122 % |
Sample type EDTA Plasma | Average % = 112 | Range 89 - 125 % |
Sample type Heparin Plasma | Average % = 106 | Range 100 - 117 % |
Sample type Cell culture supernatant | Average % = 111 | Range 104 - 115 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human Stabilin-1 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Stabilin-1 protein in Serum, Cit Plasma, EDTA Plasma, Hep Plasma, Cell culture supernatant. Quantitate Human Stabilin-1 with 33.574 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)
-Also available in a fully validated 384-well format.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
Sandwich ELISA - Human Stabilin-1 ELISA Kit (ab317782)
Example of human Stabilin-1 standard curve in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human Stabilin-1 ELISA Kit (ab317782)
Interpolated concentrations of human Stabilin-1 in serum, plasma (citrate), plasma (EDTA), and plasma (heparin).
Interpolated concentration of native Stabilin-1 was measured in duplicate at different sample concentrations. Undiluted samples are 2.5% for serum, 2.5% plasma (citrate), 5% plasma (EDTA) and 5% plasma (heparin). The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
Sandwich ELISA - Human Stabilin-1 ELISA Kit (ab317782)
Interpolated concentrations of human Stabilin-1 in HUVEC cell culture supernatant. Interpolated concentration of native Stabilin-1 was measured in duplicate at different sample concentrations. Undiluted samples are 100% HUVEC cell culture supernatant. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
Sandwich ELISA - Human Stabilin-1 ELISA Kit (ab317782)
Example of human Stabilin-1 standard curve in Sample Diluent NS in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human Stabilin-1 ELISA Kit (ab317782)
Interpolated concentrations of human Stabilin-1 in normal human serum donors. Serum of ten individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Stabilin-1 concentration was determined to be 226,774 pg/mL with a range of 183,511 - 327,540 pg/mL.
Sandwich ELISA - Human Stabilin-1 ELISA Kit (ab317782)
Interpolated concentrations of human Stabilin-1 in serum in 96-well vs. 384-well plates. Interpolated concentration of native Stabilin-1 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 5% serum. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
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Product protocols
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