Human Stanniocalcin 2 / STC-2 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human Stanniocalcin 2 / STC-2 in Human in Plasma, Serum samples.
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- Nature communications 13:13662022Electrical modulation of transplanted stem cells improves functional recovery in a rodent model of stroke.Applications:Unspecified applicationReactive species:Unspecified reactive speciesByeongtaek Oh,Sruthi Santhanam,Matine Azadian,Vishal Swaminathan,Alex G Lee,Kelly W McConnell,Alexa Levinson,Shang Song,Jainith J Patel,Emily E Gardner,Paul M GeorgePubMed 35292643
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 1.25 - 80 ng/mL
- Assay time
- 4h
- Sensitivity
- >= 70 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Has an anti-hypocalcemic action on calcium and phosphate homeostasis.
Alternative names
Stanniocalcin-2, STC-2, Stanniocalcin-related protein, STC-related protein, STCRP, STC2
What's included?
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Human Stanniocalcin 2 / STC-2 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human Stanniocalcin 2 / STC-2 in Human in Plasma, Serum samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 1.25 - 80 ng/mL
- Assay time
- 4h
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- >= 70 pg/mL
- General recovery
- 97%
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. 4.8 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. 10.7 |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- Multi
- Storage information
- Please refer to protocols
Notes
The Human Stanniocalcin 2 / STC-2 ELISA (Enzyme-Linked Immunosorbent Assay) kit (ab222880) is designed for detection of Stanniocalcin 2 / STC-2 in human plasma, serum, and cell culture samples.
This assay employs a quantitative sandwich enzyme immunoassay technique that measures human Stanniocalcin 2 / STC-2 in approximately 4 hours. A polyclonal antibody specific for human Stanniocalcin 2 / STC-2 has been pre-coated onto a 96-well microplate with removable strips. Stanniocalcin 2 / STC-2 in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for human Stanniocalcin 2 / STC-2, which is recognized by a streptavidin-peroxidase (SP) conjugate. All unbound material is washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
Stanniocalcin 2 / STC-2, also known as stanniocalcin-related protein, is a secreted homodimeric glycoprotein that belongs to the stanniocalcin family. Stanniocalcin 2 / STC-2 contains 302 amino acid residues with 34% STC-1 identity and a molecular mass of 32 kDa. The N terminus of Stanniocalcin 2 / STC-2 contains a signal peptide followed by a cysteine-rich region that may mediate homodimerization. In the kidney, the function of Stanniocalcin 2 / STC-2 on phosphate transport appears to be the opposite to that of STC-1. Stanniocalcin 2 / STC-2 is phosphorylated by casein kinase-2 on serine residues and can inhibit phosphorylation of cell-surface proteins. It is expressed in a wide variety of tissues, including the pancreas, skeletal muscle, and small intestine. Stanniocalcin 2 / STC-2 may play a role in the regulation of renal and intestinal calcium and phosphate transport, cell metabolism, and cellular calcium/phosphate homeostasis.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Stanniocalcin 2 (STC-2) is a glycoprotein hormone known for regulating calcium and phosphate homeostasis. It has other names like STC-1-related protein and STC2. The protein has a mass of approximately 33 kDa. STC-2 expresses in various tissues with high levels in the liver kidney and thyroid gland. Its expression suggests an important role in maintaining mineral balance in these tissues.
- Biological function summary
STC-2 influences metabolic and inflammatory processes by modulating cellular responses to calcium and phosphate. It operates partly by binding to receptors on cell surfaces impacting cell proliferation and survival. While not part of a known complex STC-2 interacts with signaling molecules involved in bone metabolism and endocrine function serving as a modulator of mineralized tissue homeostasis.
- Pathways
In mineral homeostasis STC-2 interacts within pathways involving calcium and phosphate regulation. Its interactions include overlap with the PTH (parathyroid hormone) and vitamin D pathways each critical for calcium and bone metabolism. STC-2 shows connections with proteins like FGF23 (fibroblast growth factor 23) that also participate in these regulatory networks impacting how cells manage mineral concentrations.
- Associated diseases and disorders
Research indicates STC-2's involvement in conditions such as chronic kidney disease and some cancers. Its expression levels correlate with abnormal mineral metabolism in kidney disease where it may interact with proteins like Klotho a known regulator of phosphate transport. In oncology overexpression of STC-2 associates with cancer progression potentially linking to altered signaling pathways in tumor cells.
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Sandwich ELISA - Human Stanniocalcin 2 / STC-2 ELISA Kit (ab222880)
Example of human Stanniocalcin 2 / STC-2 standard curve. Background-subtracted data values (mean +/- SD) are graphed.
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