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AB222880

Human Stanniocalcin 2 / STC-2 ELISA Kit

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(1 Publication)

Human Stanniocalcin 2 / STC-2 ELISA Kit is a Sandwich ELISA for the measurement of Human Stanniocalcin 2 / STC-2 in Human in Biofluids samples.

View Alternative Names

Stanniocalcin-2, STC-2, Stanniocalcin-related protein, STC-related protein, STCRP, STC2

1 Images
Sandwich ELISA - Human Stanniocalcin 2 / STC-2 ELISA Kit (AB222880)
  • sELISA

Supplier Data

Sandwich ELISA - Human Stanniocalcin 2 / STC-2 ELISA Kit (AB222880)

Example of human Stanniocalcin 2 / STC-2 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

>= 70 pg/mL

Range

1.25 - 80 ng/mL

Assay time

4h

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

The Human Stanniocalcin 2 / STC-2 ELISA (Enzyme-Linked Immunosorbent Assay) kit (ab222880) is designed for detection of Stanniocalcin 2 / STC-2 in human plasma, serum, and cell culture samples.

This assay employs a quantitative sandwich enzyme immunoassay technique that measures human Stanniocalcin 2 / STC-2 in approximately 4 hours. A polyclonal antibody specific for human Stanniocalcin 2 / STC-2 has been pre-coated onto a 96-well microplate with removable strips. Stanniocalcin 2 / STC-2 in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for human Stanniocalcin 2 / STC-2, which is recognized by a streptavidin-peroxidase (SP) conjugate. All unbound material is washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.

The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

Stanniocalcin 2 / STC-2, also known as stanniocalcin-related protein, is a secreted homodimeric glycoprotein that belongs to the stanniocalcin family. Stanniocalcin 2 / STC-2 contains 302 amino acid residues with 34% STC-1 identity and a molecular mass of 32 kDa. The N terminus of Stanniocalcin 2 / STC-2 contains a signal peptide followed by a cysteine-rich region that may mediate homodimerization. In the kidney, the function of Stanniocalcin 2 / STC-2 on phosphate transport appears to be the opposite to that of STC-1. Stanniocalcin 2 / STC-2 is phosphorylated by casein kinase-2 on serine residues and can inhibit phosphorylation of cell-surface proteins. It is expressed in a wide variety of tissues, including the pancreas, skeletal muscle, and small intestine. Stanniocalcin 2 / STC-2 may play a role in the regulation of renal and intestinal calcium and phosphate transport, cell metabolism, and cellular calcium/phosphate homeostasis.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10.7" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.8" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Stanniocalcin 2 (STC-2) is a glycoprotein hormone known for regulating calcium and phosphate homeostasis. It has other names like STC-1-related protein and STC2. The protein has a mass of approximately 33 kDa. STC-2 expresses in various tissues with high levels in the liver kidney and thyroid gland. Its expression suggests an important role in maintaining mineral balance in these tissues.
Biological function summary

STC-2 influences metabolic and inflammatory processes by modulating cellular responses to calcium and phosphate. It operates partly by binding to receptors on cell surfaces impacting cell proliferation and survival. While not part of a known complex STC-2 interacts with signaling molecules involved in bone metabolism and endocrine function serving as a modulator of mineralized tissue homeostasis.

Pathways

In mineral homeostasis STC-2 interacts within pathways involving calcium and phosphate regulation. Its interactions include overlap with the PTH (parathyroid hormone) and vitamin D pathways each critical for calcium and bone metabolism. STC-2 shows connections with proteins like FGF23 (fibroblast growth factor 23) that also participate in these regulatory networks impacting how cells manage mineral concentrations.

Research indicates STC-2's involvement in conditions such as chronic kidney disease and some cancers. Its expression levels correlate with abnormal mineral metabolism in kidney disease where it may interact with proteins like Klotho a known regulator of phosphate transport. In oncology overexpression of STC-2 associates with cancer progression potentially linking to altered signaling pathways in tumor cells.
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Product protocols

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Target data

Has an anti-hypocalcemic action on calcium and phosphate homeostasis.
See full target information Stanniocalcin-2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 13:1366 PubMed35292643

2022

Electrical modulation of transplanted stem cells improves functional recovery in a rodent model of stroke.

Applications

Unspecified application

Species

Unspecified reactive species

Byeongtaek Oh,Sruthi Santhanam,Matine Azadian,Vishal Swaminathan,Alex G Lee,Kelly W McConnell,Alexa Levinson,Shang Song,Jainith J Patel,Emily E Gardner,Paul M George
View all publications
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