Human Stanniocalcin 2 / STC-2 ELISA Kit- Extracellular is a single-wash 90-min Simplestep used to quantify Human Stanniocalcin 2 / STC-2 with a sensitivity of 17.725 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Serum, Citrate plasma, EDTA Plasma, Cell culture supernatant, Heparin Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 50 - 3200 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 17.725 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Has an anti-hypocalcemic action on calcium and phosphate homeostasis.
Alternative names
Stanniocalcin-2, STC-2, Stanniocalcin-related protein, STC-related protein, STCRP, STC2
What's included?
Recommended products
Human Stanniocalcin 2 / STC-2 ELISA Kit- Extracellular is a single-wash 90-min Simplestep used to quantify Human Stanniocalcin 2 / STC-2 with a sensitivity of 17.725 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
Key facts
- Detection method
- Colorimetric
- Sample types
- Serum, Citrate plasma, EDTA Plasma, Cell culture supernatant, Heparin Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 50 - 3200 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 17.725 pg/mL
- General recovery
- 93%
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Plasma | n 8 | C.V. 4.9 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Plasma | n 3 | C.V. 4.7 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 93 | Range 87 - 100 % |
Sample type Citrate plasma | Average % = 94 | Range 87 - 102 % |
Sample type EDTA Plasma | Average % = 87 | Range 85 - 90 % |
Sample type Heparin Plasma | Average % = 93 | Range 83 - 104 % |
Sample type Cell culture supernatant | Average % = 109 | Range 94 - 127 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human Stanniocalcin 2 / STC-2 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Stanniocalcin 2 / STC-2 protein in Serum, Cit plasma, EDTA Plasma, Hep Plasma, Cell culture supernatant. Quantitate Human Stanniocalcin 2 / STC-2 with 17.725 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)
-Also available in a fully validated 384-well format.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Stanniocalcin 2 (STC-2) is a glycoprotein hormone known for regulating calcium and phosphate homeostasis. It has other names like STC-1-related protein and STC2. The protein has a mass of approximately 33 kDa. STC-2 expresses in various tissues with high levels in the liver kidney and thyroid gland. Its expression suggests an important role in maintaining mineral balance in these tissues.
- Biological function summary
STC-2 influences metabolic and inflammatory processes by modulating cellular responses to calcium and phosphate. It operates partly by binding to receptors on cell surfaces impacting cell proliferation and survival. While not part of a known complex STC-2 interacts with signaling molecules involved in bone metabolism and endocrine function serving as a modulator of mineralized tissue homeostasis.
- Pathways
In mineral homeostasis STC-2 interacts within pathways involving calcium and phosphate regulation. Its interactions include overlap with the PTH (parathyroid hormone) and vitamin D pathways each critical for calcium and bone metabolism. STC-2 shows connections with proteins like FGF23 (fibroblast growth factor 23) that also participate in these regulatory networks impacting how cells manage mineral concentrations.
- Associated diseases and disorders
Research indicates STC-2's involvement in conditions such as chronic kidney disease and some cancers. Its expression levels correlate with abnormal mineral metabolism in kidney disease where it may interact with proteins like Klotho a known regulator of phosphate transport. In oncology overexpression of STC-2 associates with cancer progression potentially linking to altered signaling pathways in tumor cells.
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5 product images
Sandwich ELISA - Human Stanniocalcin 2 / STC-2 ELISA Kit- Extracellular (ab320044)
Interpolated concentration of native Stanniocalcin 2 / STC-2 was measured in duplicate at different sample concentrations. Undiluted samples are as follows: plasma (EDTA) 50%, plasma (heparin) 25%, and T-47D cell culture supernatant 1:100. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
Sandwich ELISA - Human Stanniocalcin 2 / STC-2 ELISA Kit- Extracellular (ab320044)
Serum of ten individual healthy human donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Stanniocalcin 2 / STC-2 concentration was determined to be 8.82 ng/mL with a range of 0.26 – 25.70 ng/mL.
Sandwich ELISA - Human Stanniocalcin 2 / STC-2 ELISA Kit- Extracellular (ab320044)
Interpolated concentration of native Stanniocalcin 2 / STC-2 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 25% plasma (EDTA). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
Sandwich ELISA - Human Stanniocalcin 2 / STC-2 ELISA Kit- Extracellular (ab320044)
Example of human Stanniocalcin 2 / STC-2 standard curve. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human Stanniocalcin 2 / STC-2 ELISA Kit- Extracellular (ab320044)
Example of human Stanniocalcin 2 / STC-2 standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
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Product protocols
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