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AB320055

Human Stanniocalcin 2 / STC-2 ELISA Kit- Intracellular

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Human Stanniocalcin 2 / STC-2 ELISA Kit- Intracellular is a single-wash 90-min Simplestep used to quantify Human Stanniocalcin 2 / STC-2 with a sensitivity of 15.005 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput

View Alternative Names

Stanniocalcin-2, STC-2, Stanniocalcin-related protein, STC-related protein, STCRP, STC2

4 Images
Sandwich ELISA - Human Stanniocalcin 2 / STC-2 ELISA Kit- Intracellular (AB320055)
  • sELISA

Supplier Data

Sandwich ELISA - Human Stanniocalcin 2 / STC-2 ELISA Kit- Intracellular (AB320055)

Interpolated concentrations of human Stanniocalcin 2 / STC-2 in T-47D cell extract

Interpolated concentration of native Stanniocalcin 2 / STC-2 was measured in duplicate at different sample concentrations. Undiluted samples are 20 µg/mL T-47D cell extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

Sandwich ELISA - Human Stanniocalcin 2 / STC-2 ELISA Kit- Intracellular (AB320055)
  • sELISA

Supplier Data

Sandwich ELISA - Human Stanniocalcin 2 / STC-2 ELISA Kit- Intracellular (AB320055)

Interpolated concentrations of human Stanniocalcin 2 / STC-2 in T-47D cell extract in 96-well vs. 384-well plates

Interpolated concentration of native Stanniocalcin 2 / STC-2 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 20 µg/mL T-47D cell extract.  The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

Sandwich ELISA - Human Stanniocalcin 2 / STC-2 ELISA Kit- Intracellular (AB320055)
  • sELISA

Supplier Data

Sandwich ELISA - Human Stanniocalcin 2 / STC-2 ELISA Kit- Intracellular (AB320055)

Example of human Stanniocalcin 2 / STC-2 standard curve in 1X Cell Extraction Buffer PTR in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed

Sandwich ELISA - Human Stanniocalcin 2 / STC-2 ELISA Kit- Intracellular (AB320055)
  • sELISA

Supplier Data

Sandwich ELISA - Human Stanniocalcin 2 / STC-2 ELISA Kit- Intracellular (AB320055)

Example of human Stanniocalcin 2 / STC-2 standard curve in 1X Cell Extraction Buffer PTR. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 15.005 pg/mL

Range

50 - 3200 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Stanniocalcin 2 / STC-2 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Stanniocalcin 2 / STC-2 protein in Cell Lysate. Quantitate Human Stanniocalcin 2 / STC-2 with 15.005 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)
-Also available in a fully validated 384-well format.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8.3" }, { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10.6" } ]
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Recovery

[ { "sample": "Cell Lysate", "range": "101 - 108 %", "average": "= 104" } ]
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What's included?

{ "values": { "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab320055-10x96Tests", "assetComponentDetails": [ { "size":"10 x 1 Unit", "name":"Plate Seals", "number":"AB320055-CMP11" }, { "size":"1 x 120 mL", "name":"Stop Solution", "number":"AB320055-CMP09" }, { "size":"1 x 120 mL", "name":"TMB Development Solution", "number":"AB320055-CMP08" }, { "size":"10 x 96 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB320055-CMP10" }, { "size":"20 x 1 Vial", "name":"Human Stanniocalcin 2 / STC-2 Lyophilized Recombinant Protein", "number":"AB320055-CMP03" }, { "size":"10 x 600 µL", "name":"10X Human Stanniocalcin 2 / STC-2 Capture Antibody", "number":"AB320055-CMP01" }, { "size":"2 x 50 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB320055-CMP05" }, { "size":"1 x 200 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB320055-CMP06" }, { "size":"10 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB320055-CMP04" }, { "size":"10 x 600 µL", "name":"10X Human Stanniocalcin 2 / STC-2 Detector Antibody", "number":"AB320055-CMP02" } ] }, "1x384Tests": { "sellingSize": "1 x 384 Tests", "publicAssetCode":"ab320055-1x384Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB320055-CMP11" }, { "size":"2 x 12 mL", "name":"Stop Solution", "number":"AB320055-CMP09" }, { "size":"2 x 12 mL", "name":"TMB Development Solution", "number":"AB320055-CMP08" }, { "size":"1 x 384 Unit", "name":"384 well CaptSure™ microplates", "number":"AB320055-CMP12" }, { "size":"2 x 1 Vial", "name":"Human Stanniocalcin 2 / STC-2 Lyophilized Recombinant Protein", "number":"AB320055-CMP03" }, { "size":"1 x 600 µL", "name":"10X Human Stanniocalcin 2 / STC-2 Capture Antibody", "number":"AB320055-CMP01" }, { "size":"1 x 50 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB320055-CMP05" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB320055-CMP04" }, { "size":"1 x 600 µL", "name":"10X Human Stanniocalcin 2 / STC-2 Detector Antibody", "number":"AB320055-CMP02" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB320055-CMP06" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab320055-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB320055-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB320055-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB320055-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB320055-CMP08", "productcode":"" }, { "size":"1 x 96 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB320055-CMP10", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Stanniocalcin 2 / STC-2 Lyophilized Recombinant Protein", "number":"AB320055-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Stanniocalcin 2 / STC-2 Capture Antibody", "number":"AB320055-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Stanniocalcin 2 / STC-2 Detector Antibody", "number":"AB320055-CMP02", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB320055-CMP05", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB320055-CMP04", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB320055-CMP06", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Stanniocalcin 2 (STC-2) is a glycoprotein hormone known for regulating calcium and phosphate homeostasis. It has other names like STC-1-related protein and STC2. The protein has a mass of approximately 33 kDa. STC-2 expresses in various tissues with high levels in the liver kidney and thyroid gland. Its expression suggests an important role in maintaining mineral balance in these tissues.
Biological function summary

STC-2 influences metabolic and inflammatory processes by modulating cellular responses to calcium and phosphate. It operates partly by binding to receptors on cell surfaces impacting cell proliferation and survival. While not part of a known complex STC-2 interacts with signaling molecules involved in bone metabolism and endocrine function serving as a modulator of mineralized tissue homeostasis.

Pathways

In mineral homeostasis STC-2 interacts within pathways involving calcium and phosphate regulation. Its interactions include overlap with the PTH (parathyroid hormone) and vitamin D pathways each critical for calcium and bone metabolism. STC-2 shows connections with proteins like FGF23 (fibroblast growth factor 23) that also participate in these regulatory networks impacting how cells manage mineral concentrations.

Research indicates STC-2's involvement in conditions such as chronic kidney disease and some cancers. Its expression levels correlate with abnormal mineral metabolism in kidney disease where it may interact with proteins like Klotho a known regulator of phosphate transport. In oncology overexpression of STC-2 associates with cancer progression potentially linking to altered signaling pathways in tumor cells.
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Product protocols

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Target data

Has an anti-hypocalcemic action on calcium and phosphate homeostasis.
See full target information Stanniocalcin-2
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websiteProtocolBooklet
en
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Product promise

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For full details, please see our Terms & Conditions

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