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AB209881

Human sTNF RI ELISA Kit

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(1 Publication)

Human sTNF RI ELISA Kit is a single-wash 90-min Simplestep used to quantify Human sTNF RI with a sensitivity of 1.9 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

CD120a, TNFAR, TNFR1, TNFRSF1A, Tumor necrosis factor receptor superfamily member 1A, Tumor necrosis factor receptor 1, Tumor necrosis factor receptor type I, p55, p60, TNF-R1, TNF-RI, TNFR-I

6 Images
ELISA - Human sTNF RI ELISA Kit (AB209881)
  • ELISA

Supplier Data

ELISA - Human sTNF RI ELISA Kit (AB209881)
Sandwich ELISA - Human sTNF RI ELISA Kit (AB209881)
  • sELISA

Supplier Data

Sandwich ELISA - Human sTNF RI ELISA Kit (AB209881)

Interpolated concentrations of native sTNF R1 in human plasma samples.

The concentrations of sTNF R1 were measured in duplicates, interpolated from the TNF R1 standard curves and corrected for sample dilution. Undiluted samples are as follows : plasma (citrate) 6.25%, plasma (EDTA) 6.25%, and plasma (heparin) 6.25%.The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean sTNF R1 concentration was determined to be 1,791 pg/mL in plasma (citrate), 2,282 pg/mL in plasma (EDTA) and 2,455 pg/mL in plasma (heparin).

Sandwich ELISA - Human sTNF RI ELISA Kit (AB209881)
  • sELISA

Supplier Data

Sandwich ELISA - Human sTNF RI ELISA Kit (AB209881)

Serum from ten individual healthy female human donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean sTNF R1 concentration was determined to be 2,043 pg/mL with a range of 1,500 – 2,509 pg/mL

Sandwich ELISA - Human sTNF RI ELISA Kit (AB209881)
  • sELISA

Supplier Data

Sandwich ELISA - Human sTNF RI ELISA Kit (AB209881)

Interpolated concentrations of native sTNF R1 in human serum and urine samples.

The concentrations of sTNF R1 were measured in duplicates, interpolated from the sTNF R1 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 6.25% and urine 6.25%.The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean sTNF R1 concentration was determined to be 2,318 pg/mL in serum and 4,082 pg/mL in urine.

Sandwich ELISA - Human sTNF RI ELISA Kit (AB209881)
  • sELISA

Supplier Data

Sandwich ELISA - Human sTNF RI ELISA Kit (AB209881)

Peripheral Blood Mononuclear Cells (PBMC) were grown in the absence (unstimulated) or presence of phytohemagglutinin M (PHA-M) for 2 days.

Human sTNF R1 was measured in 2-fold diluted cell culture supernatants of unstimulated and PHA-M stimulated PBMC and cell culture media. Measured values were interpolated from the sTNF R1 standard curve diluted in Sample Diluent NS and corrected for dilution factor. Mean of the duplicate values +/- SD are graphed : 86.1 pg/mL unstimulated, 196.4 pg/mL stimulated and undetectable in media.

Sandwich ELISA - Human sTNF RI ELISA Kit (AB209881)
  • sELISA

Supplier Data

Sandwich ELISA - Human sTNF RI ELISA Kit (AB209881)

Example of human sTNF R1 standard curve.

Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Urine, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 1.9 pg/mL

Range

15.6 - 1000 pg/mL

Assay time

1h 30m

Assay Platform

Microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human sTNF RI ELISA Kit ab209881 is a rapid single-wash 90-min Sandwich ELISA to measure Human sTNF RI in cell culture supernatant, citrate plasma, EDTA plasma, heparin plasma, serum, urine. This SimpleStep sensitivity is 1.9 pg/mL.

How the assay works

Human sTNF RI SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human sTNF RI ELISA Kit ab209881 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-Soluble TNF Receptor I antibody [EPR18822-47] - BSA and Azide free (Capture) ab242762
- Anti-Soluble TNF Receptor I antibody [EPR18822-13] - BSA and Azide free (Detector) ab242762

Tumor necrosis factor receptor superfamily member 1A (TNF RI) is a transmembrane protein with an extracellular domain that binds to TNF alpha. This extracellular domain can be proteolytically cleaved to make soluble TNF RI. The standard protein in this kit is soluble TNF RI and the capture and detector antibodies were raised against this region of TNF RI.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Human Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.1" }, { "reproducibilityType": "Intra", "sample": "Human Serum", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.3" } ]
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Recovery

[ { "sample": "Serum", "range": "87 - 110 %", "average": "= 101" }, { "sample": "EDTA Plasma", "range": "89 - 105 %", "average": "= 99" }, { "sample": "Urine", "range": "102 - 116 %", "average": "= 107" }, { "sample": "Heparin Plasma", "range": "105 - 112 %", "average": "= 109" }, { "sample": "Citrate plasma", "range": "90 - 105 %", "average": "= 98" }, { "sample": "Cell culture media", "range": "108 - 109 %", "average": "= 108" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab209881-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB209881-CMP07", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB209881-CMP10", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB209881-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB209881-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB209881-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human sTNF R1 Lyophilized Recombinant Protein", "number":"AB209881-CMP02", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human sTNF R1 Capture Antibody", "number":"AB209881-CMP04", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BI", "number":"AB209881-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB209881-CMP06", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human sTNF R1 Detector Antibody", "number":"AB209881-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Soluble TNF Receptor I + Fc Fragment also known as TNFRSF1A-Fc is a recombinant fusion protein with an approximate mass of 75 kDa. Mechanically this fusion protein acts by binding to tumor necrosis factor-alpha (TNF-α) inhibiting its activity and preventing interactions with cell surface TNF receptors. Soluble TNF Receptor I + Fc Fragment keeps the TNF-α activity in check by acting as a decoy receptor. It is expressed in various cells including immune cells and endothelial cells allowing it to modulate inflammatory responses effectively.
Biological function summary

The Soluble TNF Receptor I + Fc Fragment helps regulate immune and inflammatory responses. It functions by blocking the pro-inflammatory effects of TNF-α an element pivotal in the inflammatory process. By neutralizing TNF-α it prevents the recruitment and activation of immune cells and cytokines. This protein does not function alone; it works within larger protein complexes to execute its role in cellular communication and signal transduction.

Pathways

This fusion protein plays a significant role in the NF-κB and apoptosis signal pathways. Within these pathways TNF-α is an important mediator whose activity is moderated by the presence of the Soluble TNF Receptor I + Fc Fragment. By inhibiting TNF-induced activation of NF-κB the fusion protein can influence processes related to cell survival and death. It is closely related to proteins such as IκB (inhibitor of NF-κB) showing how its activity can have downstream effects on a variety of signaling processes.

Soluble TNF Receptor I + Fc Fragment is used therapeutically in conditions like rheumatoid arthritis and inflammatory bowel disease. These disorders involve high levels of TNF-α which contribute to excessive inflammatory responses. The fusion protein helps mitigate such conditions by decreasing the TNF-α activity which in turn lowers inflammation. Through these actions this target is also indirectly connected to the regulatory proteins involved in these disorders like interleukin-6 (IL-6) which can also influence the inflammatory process.
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Product protocols

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Target data

Receptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Contributes to the induction of non-cytocidal TNF effects including anti-viral state and activation of the acid sphingomyelinase.
See full target information TNFRSF1A
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Biology 11: PubMed35741460

2022

The Inflammatory Response after Moderate Contusion Spinal Cord Injury: A Time Study.

Applications

Unspecified application

Species

Unspecified reactive species

Minna Christiansen Lund,Ditte Gry Ellman,Maiken Nissen,Pernille Sveistrup Nielsen,Pernille Vinther Nielsen,Carina Jørgensen,Ditte Caroline Andersen,Han Gao,Roberta Brambilla,Matilda Degn,Bettina Hjelm Clausen,Kate Lykke Lambertsen
View all publications
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websiteProtocolBooklet
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