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AB326888

Human sTNF RI ELISA Kit, Chemiluminescent

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Human sTNF RI ELISA Kit, Chemiluminescent is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Human sTNF RI with higher sensitivity and broader measuring range.

- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 14.98 - 3640 pg/ml with a sensitivity of 4.35 pg/mL.
- Design your own immunoassay: we also offer the conjugation-ready antibody pair.
- Patent pending

View Alternative Names

CD120a, TNFAR, TNFR1, TNFRSF1A, Tumor necrosis factor receptor superfamily member 1A, Tumor necrosis factor receptor 1, Tumor necrosis factor receptor type I, p55, p60, TNF-R1, TNF-RI, TNFR-I

6 Images
Sandwich ELISA - Human sTNF RI ELISA Kit, Chemiluminescent (AB326888)
  • sELISA

Lab

Sandwich ELISA - Human sTNF RI ELISA Kit, Chemiluminescent (AB326888)

Dynamic range comparison between the Chemiluminescent Human sTNF R1 SimpleStep ELISA, colorimetric Human sTNF R1 SimpleStep ELISA (ab209881). The Chemiluminescent SimpleStep ELISA kit shows increased dynamic range.

Sandwich ELISA - Human sTNF RI ELISA Kit, Chemiluminescent (AB326888)
  • sELISA

Lab

Sandwich ELISA - Human sTNF RI ELISA Kit, Chemiluminescent (AB326888)

Standard curve comparison between the Chemiluminescent Human sTNF R1 SimpleStep ELISA and colorimetric Human sTNF R1 SimpleStep ELISA (ab209881) in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human sTNF RI ELISA Kit, Chemiluminescent (AB326888)
  • sELISA

Supplier Data

Sandwich ELISA - Human sTNF RI ELISA Kit, Chemiluminescent (AB326888)

Peripheral Blood Mononuclear Cells (PBMC) were grown in the absence (unstimulated) or presence of phytohemagglutinin M (PHA-M) for 2 days.

Human sTNF R1 was measured in 2-fold diluted cell culture supernatants of unstimulated and PHA-M stimulated PBMC and cell culture media. Measured values were interpolated from the sTNF R1 standard curve diluted in Sample Diluent NS and corrected for dilution factor. Mean of the duplicate values +/- SD are graphed : 86.1 pg/mL unstimulated, 196.4 pg/mL stimulated and undetectable in media.

Sandwich ELISA - Human sTNF RI ELISA Kit, Chemiluminescent (AB326888)
  • sELISA

Supplier Data

Sandwich ELISA - Human sTNF RI ELISA Kit, Chemiluminescent (AB326888)

Interpolated concentrations of native sTNF R1 in human serum and urine samples.

The concentrations of sTNF R1 were measured in duplicates, interpolated from the sTNF R1 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 6.25% and urine 6.25%.The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean sTNF R1 concentration was determined to be 2,318 pg/mL in serum and 4,082 pg/mL in urine.

Sandwich ELISA - Human sTNF RI ELISA Kit, Chemiluminescent (AB326888)
  • sELISA

Supplier Data

Sandwich ELISA - Human sTNF RI ELISA Kit, Chemiluminescent (AB326888)

Interpolated concentrations of native sTNF R1 in human plasma samples.

The concentrations of sTNF R1 were measured in duplicates, interpolated from the TNF R1 standard curves and corrected for sample dilution. Undiluted samples are as follows : plasma (citrate) 6.25%, plasma (EDTA) 6.25%, and plasma (heparin) 6.25%.The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean sTNF R1 concentration was determined to be 1,791 pg/mL in plasma (citrate), 2,282 pg/mL in plasma (EDTA) and 2,455 pg/mL in plasma (heparin).

Sandwich ELISA - Human sTNF RI ELISA Kit, Chemiluminescent (AB326888)
  • sELISA

Supplier Data

Sandwich ELISA - Human sTNF RI ELISA Kit, Chemiluminescent (AB326888)

Serum from ten individual healthy female human donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean sTNF R1 concentration was determined to be 2,043 pg/mL with a range of 1,500 – 2,509 pg/mL

Key facts

Detection method

Luminescent

Sample types

Serum, EDTA Plasma, Urine, Heparin Plasma, Citrate plasma, Cell culture media

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 4.35 pg/mL

Range

14.98 - 3640 pg/mL

Assay time

55m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human sTNF RI ELISA Kit, Chemiluminescent (ab326888) is a rapid single-wash sandwich 55-min ELISA to measure Human sTNF RI in Serum, EDTA Plasma, Urine, Heparin Plasma, Citrate plasma, Cell culture media with a sensitivity of 4.35 pg/mL.

How the assay works

Human sTNF RI ELISA Kit, Chemiluminescent (ab326888) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human sTNF RI (ab326888) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-TNF RI antibody [EPR18822-47] - BSA and Aside free (Capture) ab242514
- Anti-TNF RI antibody [EPR18822-13] - BSA and Aside free (Detector) ab242762
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Precision

[ { "reproducibilityType": "Intra", "sample": "Human Serum", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 2.3" }, { "reproducibilityType": "Inter", "sample": "Human Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 6.1" } ]
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Recovery

[ { "sample": "Serum", "range": "87 - 110 %", "average": "= 110" }, { "sample": "EDTA Plasma", "range": "89 - 105 %", "average": "= 105" }, { "sample": "Urine", "range": "102 - 116 %", "average": "= 116" }, { "sample": "Heparin Plasma", "range": "105 - 112 %", "average": "= 112" }, { "sample": "Citrate plasma", "range": "90 - 105 %", "average": "= 105" }, { "sample": "Cell culture media", "range": "108 - 109 %", "average": "= 109" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Receptor for TNFSF2/TNF and homotrimeric TNFSF1/lymphotoxin-alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Contributes to the induction of non-cytocidal TNF effects including anti-viral state and activation of the acid sphingomyelinase.
See full target information TNFRSF1A
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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