Human sTNF RII ELISA Kit (TNFRSF1B) is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human sTNF RII (TNFRSF1B) in Urine, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma samples.
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Urine, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 15.63 - 1000 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 4.88 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Version
Target data
Function
Receptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. This receptor mediates most of the metabolic effects of TNF-alpha. Isoform 2 blocks TNF-alpha-induced apoptosis, which suggests that it regulates TNF-alpha function by antagonizing its biological activity.
Alternative names
CD120b, TNFBR, TNFR2, TNFRSF1B, Tumor necrosis factor receptor superfamily member 1B, Tumor necrosis factor receptor 2, Tumor necrosis factor receptor type II, p75, p80 TNF-alpha receptor, TNF-R2, TNF-RII, TNFR-II
What's included?
Recommended products
Human sTNF RII ELISA Kit (TNFRSF1B) is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human sTNF RII (TNFRSF1B) in Urine, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Urine, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 15.63 - 1000 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 4.88 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 8 | mean - | SD - | C.V. 2.5 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 3 | mean - | SD - | C.V. 2.5 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 95 | Range 88 - 107 % |
Sample type Serum | Average % = 101 | Range 91 - 110 % |
Sample type EDTA Plasma | Average % = 90 | Range 81 - 103 % |
Sample type Urine | Average % = 103 | Range 91 - 119 % |
Sample type Heparin Plasma | Average % = 105 | Range 90 - 117 % |
Sample type Citrate plasma | Average % = 105 | Range 93 - 113 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human sTNF RII ELISA Kit (TNFRSF1B) (ab260061) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of sTNF RII (TNFRSF1B) protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human sTNF RII (TNFRSF1B) with 4.88 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Soluble Tumor Necrosis Factor Receptor II (sTNF RII), also known as TNFRSF1B, p75/p80, and CD120b, is a widely expressed receptor for membrane-associated TNF-alpha and Lymphotoxin-alpha. Its activation initiates pro-inflammatory and pro-survival responses via NFkB-dependent signaling pathways, although it may also induce apoptosis. The TNF RII extracellular domain can be proteolytically cleaved to make soluble TNF RII. The standard protein in this kit is soluble TNF RII and the capture and detector antibodies were raised against this region of TNF RII.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
Sandwich ELISA - Human sTNF RII ELISA Kit (TNFRSF1B) (ab260061)
Example of Human sTNF RII standard curve in Sample Diluent NS.
The sTNF RII standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human sTNF RII ELISA Kit (TNFRSF1B) (ab260061)
Interpolated concentrations of native sTNF RII in Human serum and plasma samples.
The concentrations of sTNF RII were measured in duplicates, interpolated from the sTNF RII standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 12.5%, plasma (citrate) 25%, plasma (EDTA) 12.5%, and plasma (heparin) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean sTNF RII concentration was determined to be 4735.93 pg/mL in serum, 3966.75 pg/mL in plasma (citrate), 5085.66 pg/mL in plasma (EDTA), and 4056.80 pg/mL in plasma (heparin).
Sandwich ELISA - Human sTNF RII ELISA Kit (TNFRSF1B) (ab260061)
Interpolated concentrations of native sTNF RII in Human PBMC cell culture supernatant samples.
The concentrations of sTNF RII were measured in duplicates, interpolated from the sTNF RII standard curves and corrected for sample dilution. Undiluted samples are as follows: Unstimulated PBMC cell culture supernatant 50%, PHA-M stimulated PBMC cell culture supernatant 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean sTNF RII concentration was determined to be 145.97 pg/mL in unstimulated PBMC supernatant and 4504.38 pg/mL PHA-M stimulated PBMC supernatant.
Sandwich ELISA - Human sTNF RII ELISA Kit (TNFRSF1B) (ab260061)
Interpolated concentrations of native sTNF RII in Human urine samples.
The concentrations of sTNF RII were measured in duplicates, interpolated from the sTNF RII standard curves and corrected for sample dilution. Undiluted samples are as follows: urine 2.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean sTNF RII concentration was determined to be 21.79 ng/mL in urine.
Sandwich ELISA - Human sTNF RII ELISA Kit (TNFRSF1B) (ab260061)
10% Serum from ten individual healthy Human male donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean sTNF RII concentration was determined to be 5427.51 pg/mL with a range of 3377.03 – 8250.55 pg/mL.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com