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AB260061

Human sTNF RII ELISA Kit (TNFRSF1B)

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Human sTNF RII ELISA Kit (TNFRSF1B) is a single-wash 90-min Simplestep used to quantify Human sTNF RII (TNFRSF1B) with a sensitivity of 4.88 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

CD120b, TNFBR, TNFR2, TNFRSF1B, Tumor necrosis factor receptor superfamily member 1B, Tumor necrosis factor receptor 2, Tumor necrosis factor receptor type II, p75, p80 TNF-alpha receptor, TNF-R2, TNF-RII, TNFR-II

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Sandwich ELISA - Human sTNF RII ELISA Kit (TNFRSF1B) (AB260061)
  • sELISA

Supplier Data

Sandwich ELISA - Human sTNF RII ELISA Kit (TNFRSF1B) (AB260061)

Interpolated concentrations of native sTNF RII in Human serum and plasma samples.

The concentrations of sTNF RII were measured in duplicates, interpolated from the sTNF RII standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 12.5%, plasma (citrate) 25%, plasma (EDTA) 12.5%, and plasma (heparin) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean sTNF RII concentration was determined to be 4735.93 pg/mL in serum, 3966.75 pg/mL in plasma (citrate), 5085.66 pg/mL in plasma (EDTA), and 4056.80 pg/mL in plasma (heparin).

Sandwich ELISA - Human sTNF RII ELISA Kit (TNFRSF1B) (AB260061)
  • sELISA

Supplier Data

Sandwich ELISA - Human sTNF RII ELISA Kit (TNFRSF1B) (AB260061)

10% Serum from ten individual healthy Human male donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean sTNF RII concentration was determined to be 5427.51 pg/mL with a range of 3377.03 – 8250.55 pg/mL.

Sandwich ELISA - Human sTNF RII ELISA Kit (TNFRSF1B) (AB260061)
  • sELISA

Supplier Data

Sandwich ELISA - Human sTNF RII ELISA Kit (TNFRSF1B) (AB260061)

Interpolated concentrations of native sTNF RII in Human urine samples.

The concentrations of sTNF RII were measured in duplicates, interpolated from the sTNF RII standard curves and corrected for sample dilution. Undiluted samples are as follows : urine 2.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean sTNF RII concentration was determined to be 21.79 ng/mL in urine.

Sandwich ELISA - Human sTNF RII ELISA Kit (TNFRSF1B) (AB260061)
  • sELISA

Supplier Data

Sandwich ELISA - Human sTNF RII ELISA Kit (TNFRSF1B) (AB260061)

Interpolated concentrations of native sTNF RII in Human PBMC cell culture supernatant samples.

The concentrations of sTNF RII were measured in duplicates, interpolated from the sTNF RII standard curves and corrected for sample dilution. Undiluted samples are as follows : Unstimulated PBMC cell culture supernatant 50%, PHA-M stimulated PBMC cell culture supernatant 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean sTNF RII concentration was determined to be 145.97 pg/mL in unstimulated PBMC supernatant and 4504.38 pg/mL PHA-M stimulated PBMC supernatant.

Sandwich ELISA - Human sTNF RII ELISA Kit (TNFRSF1B) (AB260061)
  • sELISA

Supplier Data

Sandwich ELISA - Human sTNF RII ELISA Kit (TNFRSF1B) (AB260061)

Example of Human sTNF RII standard curve in Sample Diluent NS.

The sTNF RII standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Urine, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 4.88 pg/mL

Range

15.63 - 1000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human sTNF RII ELISA Kit (TNFRSF1B) (ab260061) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of sTNF RII (TNFRSF1B) protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human sTNF RII (TNFRSF1B) with 4.88 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Soluble Tumor Necrosis Factor Receptor II (sTNF RII), also known as TNFRSF1B, p75/p80, and CD120b, is a widely expressed receptor for membrane-associated TNF-alpha and Lymphotoxin-alpha. Its activation initiates pro-inflammatory and pro-survival responses via NFkB-dependent signaling pathways, although it may also induce apoptosis. The TNF RII extracellular domain can be proteolytically cleaved to make soluble TNF RII. The standard protein in this kit is soluble TNF RII and the capture and detector antibodies were raised against this region of TNF RII.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.5" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.5" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "88 - 107 %", "average": "= 95" }, { "sample": "Serum", "range": "91 - 110 %", "average": "= 101" }, { "sample": "EDTA Plasma", "range": "81 - 103 %", "average": "= 90" }, { "sample": "Urine", "range": "91 - 119 %", "average": "= 103" }, { "sample": "Heparin Plasma", "range": "90 - 117 %", "average": "= 105" }, { "sample": "Citrate plasma", "range": "93 - 113 %", "average": "= 105" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab260061-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB260061-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB260061-CMP06", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB260061-CMP10", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB260061-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB260061-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB260061-CMP09", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB260061-CMP08", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human sTNF RII (TNFRSF1B) Capture Antibody", "number":"AB260061-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human sTNF RII (TNFRSF1B) Lyophilized Recombinant Protein", "number":"AB260061-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human sTNF RII (TNFRSF1B) Detector Antibody", "number":"AB260061-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Receptor with high affinity for TNFSF2/TNF and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. This receptor mediates most of the metabolic effects of TNF. Isoform 2 blocks TNF-induced apoptosis, which suggests that it regulates TNF function by antagonizing its biological activity.
See full target information TNFRSF1B
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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