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AB119520

Human Superoxide Dismutase 1 ELISA Kit

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(15 Publications)

Human Superoxide Dismutase 1 ELISA Kit is a sandwich ELISA designed to quantify Human Superoxide Dismutase 1 with a sensitivity of 0.04 ng/mL.

- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 0.08 - 5 ng/mL
- Cited in over 10 publications

View Alternative Names

Superoxide dismutase [Cu-Zn], Hydrogen sulfide oxidase, Superoxide dismutase 1, hSod1, SOD1

1 Images
Sandwich ELISA - Human Superoxide Dismutase 1 ELISA Kit (AB119520)
  • sELISA

Supplier Data

Sandwich ELISA - Human Superoxide Dismutase 1 ELISA Kit (AB119520)

Representative Standard Curve using ab119520.

Key facts

Detection method

Colorimetric

Sample types

Urine, Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.04 ng/mL

Range

0.08 - 5 ng/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Superoxide Dismutase 1 ELISA Kit ab119520 is a sandwich ELISA to measure Human Superoxide Dismutase 1 in serum, plasma, cell culture supernatant, urine with a sensitivity of 0.04 ng/ml.

How the assay works

Cu/Zn Superoxide Dismutase specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells along with a Cu/Zn Superoxide Dismutase HRP-conjugated detection antibody and the microplate is then incubated at room temperature. After the removal of unbound proteins by washing, TMB is added and catalyzed by HRP to produce a blue color product that changes to yellow after addition of an acidic stop solution. The density of yellow coloration is directly proportional to the Cu/Zn Superoxide Dismutase amount of sample captured in plate.

Assay Specificity

Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details

Human Superoxide Dismutase 1 ELISA Kit ab119520 protocol summary

1. Add standard or sample to appropriate wells. Incubate the plate
2. Wash and add Biotinylated mouse anti-TGF beta 1 monoclonal antibody to appropriate wells. Incubate the plate.
3. Wash and add prepared Streptavidin-HRP Conjugate to appropriate wells. Incubate at room temperature
4. Wash and add TMB Substrate to each well
5. Add Stop Solution to each well. Read immediately
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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.8" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.1" } ]
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Recovery

[ { "sample": "Serum", "range": "89 - 108 %", "average": "= 98" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab119520-1x96Tests", "assetComponentDetails": [ { "size":"1 x 5 mL", "name":"20X Assay Buffer Concentrate", "number":"AB119520-CMP02", "productcode":"" }, { "size":"1 x 50 mL", "name":"20X Wash Buffer Concentrate", "number":"AB119520-CMP09", "productcode":"" }, { "size":"2 x 500 µL", "name":"Human Cu/Zn Superoxide Dismutase Standard (5 ng/mL)", "number":"AB119520-CMP05", "productcode":"" }, { "size":"2 x 1 Unit", "name":"Adhesive Films", "number":"AB119520-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Microplate coated with anti Human Cu/Zn SOD monoclonal antibody (12 x 8 wells)", "number":"AB119520-CMP03", "productcode":"" }, { "size":"2 x 20 µL", "name":"HRP-Conjugate anti-Human Cu/Zn Superoxide Dismutase monoclonal antibody", "number":"AB119520-CMP04", "productcode":"" }, { "size":"1 x 15 mL", "name":"TMB Substrate Solution", "number":"AB119520-CMP08", "productcode":"" }, { "size":"1 x 15 mL", "name":"Stop Solution (1M Phosphoric acid)", "number":"AB119520-CMP07", "productcode":"" }, { "size":"1 x 5 mL", "name":"Phosphate Buffered Saline Concentrate (PBS) (20x)", "number":"AB119520-CMP06", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Superoxide Dismutase 1 (SOD1) also known as Cu/Zn SOD or simply dismutase is an enzyme important for the detoxification of superoxide radicals. SOD1 catalyzes the conversion of two superoxide molecules into oxygen and hydrogen peroxide maintaining cellular redox balance. This enzyme typically exhibits a mass of approximately 32 kDa. SOD1 is expressed in the cytoplasm of cells throughout the body including tissues like liver kidney and brain.
Biological function summary

The enzyme functions as a homodimer with each subunit containing a copper and zinc ion. These metal ions are essential for the catalytic activity of SOD1 as the copper ion participates in electron transfer while the zinc ion provides structural stability. The enzyme protects cells from oxidative stress by neutralizing excess reactive oxygen species ensuring cellular health and functioning.

Pathways

SOD1 plays an important role in the cellular antioxidant defense system and is a part of the reactive oxygen species (ROS) metabolic pathway. It works in conjunction with catalase and glutathione peroxidase to limit oxidative damage within cells. The close interaction between these enzymes highlights the interdependence within the antioxidant defense network emphasizing their role in maintaining cellular homeostasis.

Mutations in the SOD1 gene are linked to familial Amyotrophic Lateral Sclerosis (ALS) a neurodegenerative condition. In ALS aberrant SOD1 proteins may lead to increased oxidative stress and motor neuron damage. Research also connects SOD1 to the pathogenesis of Alzheimer's disease where oxidative stress is a contributing factor. These associations underline SOD1's significance in neurodegenerative diseases highlighting potential therapeutic targets for interventions focused on oxidative stress management.
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Product protocols

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Target data

Destroys radicals which are normally produced within the cells and which are toxic to biological systems (PubMed : 24140062). Catalyzes the oxidation of hydrogen sulfide (H2S) to sulfate, playing an important role in detoxifying H2S and limiting the accumulation of reactive sulfur species (RSS) such as persulfides and polysulfides (PubMed : 36630448).
See full target information SOD1
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Publications (15)

Recent publications for all applications. Explore the full list and refine your search

Histology and histopathology :18927 PubMed40351154

2025

Lysophosphatidylcholine negatively reverses the effects of human umbilical cord-derived mesenchymal stem cells on high glucose-induced cell dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Xian Liu,Yong Chai,Han Liu,Yao Zhao,ChunYi Liu,Rui Luo,Qiang Gan

Histology and histopathology 39:959-967 PubMed38193235

2024

Notoginsenoside Fc alleviates oxidized low-density lipoprotein-induced endothelial cell dysfunction and upregulates PPAR-γ .

Applications

Unspecified application

Species

Unspecified reactive species

Yuanhao Zhang,Kui Liu,Dile Wang

Science advances 9:eadg3193 PubMed37992159

2023

RGMa collapses the neuronal actin barrier against disease-implicated protein and exacerbates ALS.

Applications

Unspecified application

Species

Unspecified reactive species

Mikito Shimizu,Naoyuki Shiraishi,Satoru Tada,Tsutomu Sasaki,Goichi Beck,Seiichi Nagano,Makoto Kinoshita,Hisae Sumi,Tomoyuki Sugimoto,Yoko Ishida,Toru Koda,Teruyuki Ishikura,Yasuko Sugiyama,Keigo Kihara,Minami Kanakura,Tsuneo Nakajima,Shuko Takeda,Masanori P Takahashi,Toshihide Yamashita,Tatsusada Okuno,Hideki Mochizuki

Nutrients 15: PubMed37049398

2023

Combined Citrulline and Glutathione Supplementation Improves Endothelial Function and Blood Pressure Reactivity in Postmenopausal Women.

Applications

Unspecified application

Species

Unspecified reactive species

Arturo Figueroa,Arun Maharaj,Yejin Kang,Katherine N Dillon,Mauricio A Martinez,Masahiko Morita,Dai Nogimura,Stephen M Fischer

Molecules (Basel, Switzerland) 27: PubMed36500327

2022

HY7015 and Turcz. Extract Promotes Human Dermal Papilla Cell Cytoprotective Effect and Hair Regrowth Rate in C57BL/6 Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Hayera Lee,Hyeonji Kim,Ji-Hyun Kim,Soo-Dong Park,Jae-Jung Shim,Jeong-Lyoul Lee

The journal of obstetrics and gynaecology research 49:128-140 PubMed36288911

2022

Combining curcumin and aspirin ameliorates preeclampsia-like symptoms by inhibiting the placental TLR4/NF-κB signaling pathway in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Yaru Ju,Yan Feng,Yanjing Yang,Xiaolin Hou,Xiaofeng Zhang,Xihui Zhu,Yage Wang,Meiliu Yang

Antioxidants (Basel, Switzerland) 11: PubMed36290630

2022

Glucose Starvation-Caused Oxidative Stress Induces Inflammation and Autophagy in Human Gingival Fibroblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Runbo Li,Hirohito Kato,Yoichiro Taguchi,Xin Deng,Emika Minagawa,Takaya Nakata,Makoto Umeda

American journal of translational research 14:6638-6646 PubMed36247290

2022

Treatment effects of monosialotetrahexosylganglioside on severe traumatic brain injury in adults.

Applications

Unspecified application

Species

Unspecified reactive species

Hanqing Chu,Jindan Gao

Journal of immunology research 2021:2389029 PubMed34692851

2021

Lysophosphatidylcholine Offsets the Protective Effects of Bone Marrow Mesenchymal Stem Cells on Inflammatory Response and Oxidative Stress Injury of Retinal Endothelial Cells via TLR4/NF-B Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Haijun Zhao,Yanhui He

Journal of investigative medicine : the official publication of the American Federation for Clinical Research 69:1330-1338 PubMed34006573

2021

Hyperbaric oxygen treatment impacts oxidative stress markers in patients with necrotizing soft-tissue infection.

Applications

Unspecified application

Species

Unspecified reactive species

Morten Hedetoft,Peter Østrup Jensen,Claus Moser,Julie Vinkel,Ole Hyldegaard
View all publications
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