JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB314351

Human Superoxide Dismutase 1 ELISA Kit

Be the first to review this product! Submit a review

|

(0 Publication)

Human Superoxide Dismutase 1 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human Superoxide Dismutase 1 in Human in Cell Culture Media, Biofluids samples.

View Alternative Names

Superoxide dismutase [Cu-Zn], Superoxide dismutase 1, hSod1, SOD1

1 Images
Sandwich ELISA - Human Superoxide Dismutase 1 ELISA Kit (AB314351)
  • sELISA

Supplier Data

Sandwich ELISA - Human Superoxide Dismutase 1 ELISA Kit (AB314351)

Example of standard curve using ab314351. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Plasma, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 49 pg/mL

Range

49 - 12000 pg/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style
left-column

Product details

Human Superoxide Dismutase 1 ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Superoxide Dismutase 1 in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human Superoxide Dismutase 1 coated on a 96-well plate.
style
left-column

Recovery

[ { "sample": "Serum", "range": "85 - 146 %", "average": "= 117.1" }, { "sample": "Plasma", "range": "74 - 142 %", "average": "= 103.7" }, { "sample": "Cell culture media", "range": "72 - 135 %", "average": "= 100.3" } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab314351-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB314351-CMP07", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB314351-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human Superoxide Dismutase 1 Protein Standard", "number":"AB314351-CMP10", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human Superoxide Dismutase 1 Antibody-coated ELISA Plate", "number":"AB314351-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human Superoxide Dismutase 1 Detection Antibody", "number":"AB314351-CMP09", "productcode":"" }, { "size":"1 x 200 µL", "name":"700X HRP-Streptavidin", "number":"AB314351-CMP03", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent (Item E2)", "number":"AB314351-CMP05", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB314351-CMP04", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Superoxide Dismutase 1 (SOD1) also known as Cu/Zn SOD or simply dismutase is an enzyme important for the detoxification of superoxide radicals. SOD1 catalyzes the conversion of two superoxide molecules into oxygen and hydrogen peroxide maintaining cellular redox balance. This enzyme typically exhibits a mass of approximately 32 kDa. SOD1 is expressed in the cytoplasm of cells throughout the body including tissues like liver kidney and brain.
Biological function summary

The enzyme functions as a homodimer with each subunit containing a copper and zinc ion. These metal ions are essential for the catalytic activity of SOD1 as the copper ion participates in electron transfer while the zinc ion provides structural stability. The enzyme protects cells from oxidative stress by neutralizing excess reactive oxygen species ensuring cellular health and functioning.

Pathways

SOD1 plays an important role in the cellular antioxidant defense system and is a part of the reactive oxygen species (ROS) metabolic pathway. It works in conjunction with catalase and glutathione peroxidase to limit oxidative damage within cells. The close interaction between these enzymes highlights the interdependence within the antioxidant defense network emphasizing their role in maintaining cellular homeostasis.

Mutations in the SOD1 gene are linked to familial Amyotrophic Lateral Sclerosis (ALS) a neurodegenerative condition. In ALS aberrant SOD1 proteins may lead to increased oxidative stress and motor neuron damage. Research also connects SOD1 to the pathogenesis of Alzheimer's disease where oxidative stress is a contributing factor. These associations underline SOD1's significance in neurodegenerative diseases highlighting potential therapeutic targets for interventions focused on oxidative stress management.
style
left-column

Product protocols

style
left-column

Target data

Destroys radicals which are normally produced within the cells and which are toxic to biological systems.
See full target information SOD1
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com